FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007740243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007740243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9769875
Sequences flagged as poor quality0
Sequence length31
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA236437224.20063716270679Illumina Single End Adapter 1 (95% over 24bp)
TACCCTGTAGATCCGAATTTGTATCTCGTAT5699865.834117632006551No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT3132853.206642869023401No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA2677872.7409460202919687No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA1619091.6572269348379587No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG1137921.1647231924666384No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT950810.9732059007919753No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT907810.9291930551823846No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA870020.8905129287733978Illumina Single End Adapter 1 (95% over 23bp)
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT830180.8497345155388376No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG800340.8191916477948797No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA702120.718658119986182No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC701580.7181054005296895No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT652650.6680228764441715No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT643720.6588825343210635No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG634140.6490768817410663No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA617660.6322087027725534No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT573310.5868140585217314No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT549700.5626479356184189No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT535760.548379585204519No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT486680.498143527936642No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG413930.4236799344925089No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT408840.41847004183779224No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG301470.30857098990519327No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT294950.30189741424532046No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC294530.3014675213347151No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT288800.2956025537685999No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT259040.2651415703885669No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC247090.25291009352729693No Hit
TTGTAGTGTGCTGATGATCTCGTATGCCGTC245680.251466881613122No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA212450.21745416394784992No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT203070.20785322227766476No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT202620.20739262273058767No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCATC193260.1978121521513837No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT191660.1961744648728873No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG191390.19589810514464104No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA188820.1932675699535562Illumina Paired End PCR Primer 2 (95% over 24bp)
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA177960.18215176755076193Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTGAATCTCGT176860.18102585754679565No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT155640.15930603001573715No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT154430.15806752901137425No Hit
TATTGCACTTGTCCCGGCCTGTAATATCTCG153070.15667549482465232No Hit
TACCCTGTAGATCCGAATTTGAATCTCGTAT151940.15551887818421423No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT149730.15325682263079107No Hit
TATTGCACTTGTCCCGGCCTGTAAAATCTCG147290.15075934953108408No Hit
ACCCTGTAGATCCGAATTTGATCTCGTATGC145440.1488657736153226No Hit
CTCGTACGACTCTTAGCATCTCGTATGCCGT143280.14665489578935248No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA141170.14449519569083535No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG139650.14293939277626377No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA139230.14250949986565847No Hit
TGTCGCTGATGCCATCACCGCAGCGCTCTGA134370.13753502475722565No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT133430.13657288348110902No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGTA133200.13633746593482515No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG129450.13249913637584923No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC129120.13216136337465936No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG129070.13211018564720634No Hit
CTCGTACGACTCTTAGCGGATCTCGTATGCC125990.12895763763610077No Hit
TTCCGGCTCGAAGGACCAATCTCGTATGCCG123720.126634168809734No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTATG123040.12593815171637304No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCATC122510.12539566780537112No Hit
CACCATGATGGAACTGAGGATCTGAGGATCT121490.12435164216532965No Hit
ATGCAGTGTGGAACACAATGAACTGAACATC121320.12417763789198942No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT118940.12174157806522602No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA118770.12156757379188578No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG117640.12041095715144769No Hit
TGTCAGTTTGTCAAATACCCCAGATCTCGTA114580.11727888023132334No Hit
TACCCTGTAGATCCGAATTTGTGTATCTCGT112700.11535459767909005No Hit
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGA106150.10865031538274542No Hit
GAGACTCTGAAATCTGATTATCTCGTATGCC103160.10558988728105528No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT102090.10449468391356082No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA101760.10415691091237095No Hit
AGTCTGTGATGAATTGCTTTGACTTCTGACA100420.1027853478166302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)