FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007740244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007740244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6653287
Sequences flagged as poor quality0
Sequence length31
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA149973822.541309280660823Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT2740024.118295212576881No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1843522.7708409392229734No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1767402.6564313248474027No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA1764882.652643723320518No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT1128381.6959737344864274No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT610510.9176065905468982No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA571770.8593797321534454No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT567030.8522554340433534No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT464490.6981361242946531No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA461220.693221260408577Illumina Single End Adapter 1 (95% over 23bp)
GCGGGTGATGCGAACTGGAGTCTGAGCATCT436980.6567881409594987No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT422560.635114643333438No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATGC383320.5761362767005241No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT379010.5696582756763687No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT361250.5429647030107073No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT356840.5363364003386596No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT314720.47302934624644927No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT292680.4399028630510002No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT285950.42978756214785263No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC284350.4273827357815768No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC282130.4240460391983692No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG280390.4214307905250443No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT248500.37349959501220975No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG239840.360483472304742No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT238260.35810870626804464No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG232270.34910563755929963No Hit
TCCTGTACTGAGCTGCCCCGAATCTCGTATG223230.3355183685898414No Hit
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA205730.3092155802086998No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA189190.2843556876473238No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT189110.28423544632901No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT178980.26900988939752635No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC173950.2614497165085468No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT171700.2580679294309715No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT158390.2380627800965147No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT157700.23702569872605825No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG152180.22872904776240674No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT148970.22390436486506593No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA143800.21613376966903727No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT142910.21479608500279637No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG139930.2103170958956077No Hit
CTCGTACGACTCTTAGCATCTCGTATGCCGT137610.20683009766450777No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG133330.20039718713472005No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA131460.1975865463191352No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG123490.18560750498212386No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT122350.18389406619615237No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA115640.17380882562258324No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT114420.17197514551829796No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT111800.16803724234352135No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT110640.16629374322797139No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG107920.16220553840530252No Hit
CTCGTACGACTCTTAGCGGATCTCGTATGCC102910.15467542584590144No Hit
CGGCGGCGACTCTGGACGCGAGCATCTCGTA102710.15437482255011695No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA102610.15422452090222472No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA99750.14992589377250673Illumina Single End Adapter 1 (95% over 24bp)
CGCGACCTCAGATCAGACGATCTCGTATGCC99320.14927959668657012No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT98680.1483176661400598No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA97270.14619841290477925Illumina Paired End PCR Primer 2 (95% over 24bp)
TACAGTACTGTGATAACTGAATCTCGTATGC94840.14254608286099787No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC86750.1303866795465159No Hit
TTCACCACCTTCTCCACCCAGCATCTCGTAT86610.13017625723946674No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCATC85900.12910911553943186No Hit
TTTGGCAATGGTAGAACTCAATCTCGTATGC85510.12852293911265214No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT84410.1268696209858375No Hit
ACTTAACTTGACCGCTCTGACCAATCTCGTA82180.12351789423784064No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT80910.12160906330960922No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA78050.11731043617989123No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG77740.1168445010714253No Hit
CGGCGGCGGCGACTCTGGACGCGAGCATCTC77320.1162132341502779No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTAT76800.11543166558123828No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT74720.11230539130507973No Hit
TAACTTGACCGCTCTGACCAATCTCGTATGC74080.11134346075856942No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT73840.11098273680362804No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT73300.11017110790500996No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA71930.1081119753288863No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG70840.10647368736686091No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA69310.10417407215410968No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA68590.10309190028928558No Hit
CGCGACCTCAGATCAGACGTATCTCGTATGC68460.10289650814702567No Hit
TGTCAGTTTGTCAAATACCCCAATATCTCGT67950.10212996974277525No Hit
AAACCGTTACCATTACTGAGATCTCGTATGC67910.10206984908361838No Hit
AACTTAACTTGACCGCTCTGACCAATCTCGT67240.10106282804274037No Hit
ATTGCACTTGTCCCGGCCTGTATCTCGTATG67160.1009425867244266No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)