FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007740245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007740245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7021782
Sequences flagged as poor quality0
Sequence length31
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA219177031.21387135060587Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT842061.1992112543511033No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA678100.9657092743693837Illumina Single End Adapter 1 (95% over 23bp)
GGTAGCGTGGCCGAGCATCTCGTATGCCGTC607920.8657631353408579No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA565750.8057071552491946No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGT551030.7847438157436388No Hit
CTGTCTGAGCGTCGCTATCTCGTATGCCGTC532560.7584399515678498No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG480160.6838150201757901No Hit
CTCGTACGACTCTTAGCATCTCGTATGCCGT476610.6787593234879693No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT364430.5189993081528307No Hit
CTCGTACGACTCTTAGCGGTGGATCATCTCG307550.4379942299547323No Hit
GTTTCCGTAGTGTAGTGGTCATCTCGTATGC298100.4245361077857444No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT289360.4120891249543207No Hit
GTCGCTATCTCGTATGCCGTCTTCTGCTTGA286580.40813001599878773RNA PCR Primer, Index 31 (96% over 28bp)
TTCACCACCTTCTCCACCCAGCATCTCGTAT283570.40384335486348055No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT278620.39679386229877256No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC271920.3872521248879558No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG265170.3776391804815359No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT255750.3642237825099099No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA251960.3588262922431941No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT246050.3504096253629065No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGTA228970.32608531566488397No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT218760.3115448471627288No Hit
TACCTGGTTGATCCTGCCAGTATCTCGTATG212540.30268669690970185No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA208620.297104068454418No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT206110.29352947727514184No Hit
TCGTACGACTCTTAGCATCTCGTATGCCGTC200100.28497039640364796No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT193740.2759128665629323No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT187050.2663853705512362No Hit
CTCGACACAAGGGTTTGTATCTCGTATGCCG183070.26071729370122854No Hit
TCGTACGACTCTTAGCGGTGGATCATCTCGT182730.2602330861311274No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT182040.25925042959180444No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT176350.2511470734921705No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG168080.23936943641941605No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT167250.23818740029240443No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT165940.23632177700760293No Hit
GTACGACTCTTAGCGGTGGATCACTCGTATG156800.22330513821135434No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA155940.22208037788698085No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA152280.21686802580883316Illumina Single End Adapter 1 (95% over 24bp)
CGCGACCTCAGATCAGACGATCTCGTATGCC140890.2006470722104446No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA139720.1989808285133318No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA138380.19707248103116845No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA135150.1924725091152075No Hit
GTTTCCGTAGTGTAGTGGTTATCTCGTATGC134160.19106261060226593No Hit
CGACTCTTAGCGGTGGATCACTCGTATGCCG130600.18599267251532445No Hit
CTCGTACGACTCTTAGCGATCTCGTATGCCG130190.18540877515137894No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT129970.18509546437072527No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT128080.1824038399369277No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA124770.17768993682800177Illumina Paired End PCR Primer 2 (95% over 24bp)
TACCTGGTTGATCCTGCCATCTCGTATGCCG122930.17506951938980733No Hit
CTCGTACGACTCTTAGCGGATCTCGTATGCC120560.1716943077982199No Hit
AGTCTGTGATGAATTGCTTTGACTTCTGACA114980.16374760708891276No Hit
AATGACACGATCACTCCCGTTGAATCTCGTA110740.15770925386176898No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCG105020.14956317356477317No Hit
GGCTTTGGTGACTCTAGATAATCTCGTATGC98670.14051988512317815No Hit
CGCGACCTCAGATCAGACATCTCGTATGCCG97860.13936633179440774No Hit
CCTGTCTGAGCGTCGCTATCTCGTATGCCGT96000.13671743155797203No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT95490.1359911202028203No Hit
CTCGTACGACTCTTAGCGGTGGATCTCGTAT94480.1345527388916375No Hit
GGTAGCGTGGCCGAGCGGTCATCTCGTATGC93940.1337837033391239No Hit
GAGACTCTGAAATCTGATTATCTCGTATGCC92900.13230259783057918No Hit
AAGAACGAAAGTCGGAGGATCTCGTATGCCG86270.12286055021360676No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT84880.12088099573584028No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC84490.120325581170136No Hit
CGCGACCTCAGATCAGACGTATCTCGTATGC81480.11603892003482875No Hit
GGTAGCGTGGCCGAGCGGTCTATCTCGTATG80450.1145720559254047No Hit
TCGTACGACTCTTAGCGGTGATCTCGTATGC79950.11385998596937358No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT79600.11336153700015181No Hit
CTGTCTGAGCGTCGCTTATCTCGTATGCCGT78490.11178074169776275No Hit
CTCGTACGACTCTTAGCGGTGGAATCTCGTA75910.10810646072464226No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC75150.107024114391475No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA70760.10077214017752188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)