FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007740246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007740246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9901777
Sequences flagged as poor quality0
Sequence length31
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA209201221.127642038393716Illumina Single End Adapter 1 (95% over 24bp)
TACCCTGTAGATCCGAATTTGTATCTCGTAT5910935.969564856893869No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT3182463.2140291586045615No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA2821332.8493168448451223No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT2591442.617146397055801No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT1892171.9109398242355893No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1531781.5469748510797607No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1243541.2558755867759899No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1121111.132231113667779No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT1040291.0506094007166593No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA1016511.0265935094276513No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA974920.9845909476652525No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG846900.8553010232405759No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT746990.7543999425557655No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC713250.7203252507100493No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA712380.7194466205409392Illumina Single End Adapter 1 (95% over 23bp)
ACCCTGTAGATCCGAATTTGTGAATCTCGTA658040.6645675821622725No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT627620.6338458238354591No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT461300.4658759735752482No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG456310.460836474099548No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG408610.4126633027586866No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT402500.40649269318022413No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT401560.4055433686296914No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT382030.3858196362127727No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT348470.3519267299192862No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT348390.3518459363405175No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT340630.34400895919994967No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG296990.2999360619816019No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA286090.2889279368743611No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG284300.28712018054941046No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA276950.27969727050003246No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT269050.27171890459661935No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA265340.26797210238121905No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA263540.2661542468589224No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGTA259510.2620842703284471No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT253520.2560348511181377No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT251380.25387362288607385No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT227080.22933257333506907No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT217930.2200918077633944No Hit
TACCCTGTAGATCCGAATTTGTGTATCTCGT216650.21879911050309456No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT206520.20856862359150283No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT196410.19835833507460326No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA190410.19229881666694776No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT189540.1914201864978377No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT188520.19039006836853628No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA184600.18643118300886802Illumina Single End Adapter 1 (95% over 24bp)
TGTAAACATCCTTGACTGGAAGCATCTCGTA175390.1771298222531168No Hit
TACCCTGTAGATCCGAATTTGTGATCTCGTA157030.1585876959256909No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC155420.15696172515297002No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCG153320.15484089371029058No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT149850.15133647223119648No Hit
CTCGTACGACTCTTAGCATCTCGTATGCCGT148190.1496600054717451No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT145420.1468625278068775No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG145390.1468322302148392No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG145030.1464686591103799No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA141370.14277235288171003No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG140350.1417422347524086No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA137940.13930832819200029No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT137740.13910634424507842No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTAT136970.13832870604942932No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA135270.13661184250059358Illumina Paired End PCR Primer 2 (95% over 24bp)
TAGCTTATCAGACTGATGTTGATCTCGTATG128060.12933032121406088No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT127340.1286031790051422No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC125860.12710849779792052No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG120180.12137215370533996No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTAT119510.12069550748315175No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA119330.1205137219309221No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTAT116560.11771624426605448No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGTA112350.11346448218334951No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA110160.11125275796455526No Hit
CTCGTACGACTCTTAGCGGATCTCGTATGCC108700.10977827515202573No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT107140.10820280036603531No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT106440.10749585655180884No Hit
AGTCTGTGATGAATTGCTTTGACTTCTGACA105050.10609206812070197No Hit
TAGCTTATCAGACTGATGTTGACAATCTCGT102090.1031027057062586No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCAT101770.10277953139118362No Hit
CGCGACCTCAGATCAGACGATCTCGTATGCC101150.10215338115572589No Hit
CTGTCTGAGCGTCGCTATCTCGTATGCCGTC99840.10083038630338778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)