FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007740247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007740247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11981647
Sequences flagged as poor quality0
Sequence length31
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA192604516.07496031221751Illumina Single End Adapter 1 (95% over 24bp)
TACCCTGTAGATCCGAATTTGTATCTCGTAT5486804.579337047736425No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT5384934.494315347464335No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA4864434.059900946839779No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG2563852.1398143343732294No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT2229361.860645702548239No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA1989231.660230851401314No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA1749391.4600580370962357No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT1420521.1855799123442712No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG1312971.095817628411186No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1273111.0625500818042795No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG1056060.8813980248291408No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1032700.8619015399135028No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1013190.8456183027258273No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT983030.8204464711737877No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT965000.8053984564893291No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA958370.7998649935188377No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA892820.7451563211635261No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT838470.6997952785622878No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC772880.6450532218149976No Hit
ACCCTGTAGATCCGAATTTGATCTCGTATGC725550.6055511400060443No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT668120.5576194992224358No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA583340.4868612804233007Illumina Single End Adapter 1 (95% over 23bp)
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT520650.4345395920944758No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT512230.42751217758293164No Hit
TACCCTGTAGATCCGAATTTGAATCTCGTAT484560.40441852443157433No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC474290.39584708179100914No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT461680.3853226522196823No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT452720.3778445484164239No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA374250.3123527174519496No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT363630.30348916138156967No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT353610.29512637119087215No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG335030.27961932111670457No Hit
AACATTCATTGCTGTCGGTGGGAATCTCGTA321150.2680349371000498No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT311280.2597973383792729No Hit
TTGTAGTGTGCTGATGATCTCGTATGCCGTC306650.2559330950077231No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT287050.23957474293809525No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT277230.2313788747072919No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG255760.21345980231265368No Hit
CTCGTACGACTCTTAGCATCTCGTATGCCGT231490.1932038224794972No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT215040.1794744912782024No Hit
CTCGTACGACTCTTAGCGGATCTCGTATGCC209700.17501767494902828No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG205610.1716041208691927No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC204190.17041897495394415No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG199500.16650465499442607No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG193530.16152203449158534No Hit
AACATTCATTGCTGTCGGTGGGATCTCGTAT191810.16008650563649557No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCATC190390.158901359721247No Hit
TATTGCACTTGTCCCGGCCTGTGAATCTCGT190000.15857586189945339No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG187980.15688995010452236No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT187630.15659783667470759No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT186810.15591345663914152No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT185020.15441950509808877No Hit
AACATTCATTGCTGTCGGTGGGTATCTCGTA179160.14952869167318986No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA169550.14150809150027538Illumina Single End Adapter 1 (95% over 24bp)
TACCCTGTAGATCCGAATTTATCTCGTATGC167670.13993902507727027No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA167130.13948833578555603No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTATG163330.13631681854756697No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT160190.13369614377722863No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTAT158860.13258611274393245No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTAT154740.1291475203701127No Hit
TGAGGTAGTAGGTTGTGTGAATCTCGTATGC150600.1256922358003036No Hit
CTCGTACGACTCTTAGCGATCTCGTATGCCG147940.12347217373371124No Hit
GAGACTCTGAAATCTGATTATCTCGTATGCC145800.12168610876284372No Hit
TACCCTGTAGATCCGAATTTGTGATCTCGTA145590.12151084070495483No Hit
CACCCGTAGAACCGACCTTGATCTCGTATGC145450.12139399533302893No Hit
TGTAAACATCCCCGACTGGAAGAATCTCGTA145400.12135226484305539No Hit
TATTGCACTTGTCCCGGCCTGAATCTCGTAT145240.12121872727514005No Hit
TCGTATCCCACTTCTGACACCAATCTCGTAT144030.12020884941778037No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCATC142050.11855632201482817No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG141660.11823082419303457No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA139020.11602745432243164Illumina Paired End PCR Primer 2 (95% over 24bp)
CTTTCAGTCGGATGTTTACAGATCTCGTATG137110.11443334960544238No Hit
TTTCCGGCTCGAAGGACCAATCTCGTATGCC127690.10657132529442738No Hit
TCGTATCCGGCTCGAAGGACCAATCTCGTAT127380.10631259625659144No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC122270.1020477401812956No Hit
ATGCAGTGTGGAACACAATGAACTGAACATC121530.10143012892968721No Hit
AACATTCAACGCTGTCGGTGAATCTCGTATG120530.1005955191302164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)