FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007740248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007740248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15880389
Sequences flagged as poor quality0
Sequence length31
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA229051214.423525771314544Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT9172165.775777910730021No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT6666234.197775004126159No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA6160393.879243764116861No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA3820802.405986402474146No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC3407632.145810156161792No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG2076651.3076820725235383No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA2048821.2901573128970583No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG1959811.2341070486371586No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1548620.9751776231677951No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT1520190.9572750390434391No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1311200.8256724693582758No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT1269530.7994325579807899No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT1050350.6614132689066999No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA972620.6124661052068687No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT947060.5963707815973526No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT870710.548292614242636No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT836490.5267440237137768No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG819810.516240502672825No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA767110.483054917609386Illumina Single End Adapter 1 (95% over 23bp)
TTCCGGCTCGAAGGACCAATCTCGTATGCCG759100.4780109605627419No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA741140.46670141392632136No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT717150.4515947310862473No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT708490.4461414641669042No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT707730.4456628864695947No Hit
CACCCGTAGAACCGACCTTGCGAATCTCGTA707730.4456628864695947No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT562150.35399006913495634No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT488050.30732874364727464No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT485570.305767069056054No Hit
TTTGGCAATGGTAGAACTCACACTGATCTCG476920.30032009921167546No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA470470.29625848585950887No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG455590.2868884383121849No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT438840.27634083774648094No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT437490.27549073262626No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT436560.27490510465455226No Hit
GTACAGTACTGTGATAACTGAAATCTCGTAT435570.27428169423305687No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT425930.26821131396718306No Hit
TATTGCACTTGTCCCGGCCTGTAATATCTCG417990.2632114364452911No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT370710.23343886601266506No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG353600.22266457074823545No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT346350.21809919139890088No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT346270.2180488147991841No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA342070.21540404331405233No Hit
TTTCCGGCTCGAAGGACCAATCTCGTATGCC328540.20688410088694928No Hit
CTTCTCACTACTGCACTTGACTAGATCTCGT326230.20542947657012683No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT325330.2048627398233129No Hit
TATGGCACTGGTAGAATTCACTGATCTCGTA323620.2037859400043664No Hit
ATCCCACCAGAGTCGCCAATCTCGTATGCCG321980.2027532197101721No Hit
CTAAGCCAGGGATTGTGGGTATCTCGTATGC317550.19996361550085454No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA313820.19761480653905897No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA313820.19761480653905897No Hit
CGTACCGTGAGTAATAATGCGATCTCGTATG311910.19641206522082047No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT307690.19375469958575953No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT304990.19205448934531769No Hit
CAAAGTGCTTACAGTGCAGGTAGAATCTCGT300440.18918932023642496No Hit
TCGTATCCCACTCCTGACACCAATCTCGTAT298940.188244758991735No Hit
ATGGCACTGGTAGAATTCACTGATCTCGTAT293760.18498287416007253No Hit
TGGTTAGTACTCTGCGATCTCGTATGCCGTC292420.1841390661148162No Hit
TGGTGTAATGGTTAGCACTCTGGATCTCGTA280940.17691002405545606No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT278660.1754742909635274No Hit
TCGTATCCCACTTCTGACACCAATCTCGTAT267920.16871123245154762No Hit
AAGTTTCTCTGAACGTGTAGAGCATCTCGTA265100.1669354573115306No Hit
TACAGTACTGTGATAACTGAAAATCTCGTAT264020.16625537321535386No Hit
TGGTTAGCACTCTGGAATCTCGTATGCCGTC250000.1574268741149855No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA243240.15317005143891627No Hit
ATCCGGCTCGAAGGACCAATCTCGTATGCCG241530.15209325161996975No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA234880.14790569676851115No Hit
TCGTATCCGGCTCGAAGGACCAATCTCGTAT233920.1473011775719096No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA226530.14264763917307063Illumina Single End Adapter 1 (95% over 24bp)
CACCCGTAGAACCGACCTTGCGTATCTCGTA221600.13954318121552312No Hit
ATGGTTAGCACTCTGGAATCTCGTATGCCGT221240.13931648651679754No Hit
TTTGGCAATGGTAGAACTCACACATCTCGTA218800.1377800002254353No Hit
ATCCGGCTCGGAGGACCAATCTCGTATGCCG217700.13708732197932935No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG217460.13693619218017897No Hit
CAAGTCACTAGTGGTTCCGTTTAGATCTCGT201520.1268986546866075No Hit
TCCGGCTCGAAGGACCAATCTCGTATGCCGT194540.12250329636131708No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT192860.12144538776726439No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA190570.12000335760037112No Hit
TGAGGTAGGAGGTTGTATAGTTATCTCGTAT189290.1191973320049024No Hit
TTTGGCAATGGTAGAACTCACACTGGATCTC188260.11854873328354866No Hit
CTCGTACGACTCTTAGCATCTCGTATGCCGT186410.11738377441509777No Hit
TGTAAACATCCTTGACTGGAAGCTATCTCGT184490.11617473602189468No Hit
TCCCCGGCACCTCCACCAATCTCGTATGCCG182280.11478308245471822No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT179280.11289395996533838No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG178880.1126420769667544No Hit
TAAAGTGCTTATAGTGCAGGTAGAATCTCGT169390.10666615282534957No Hit
TATGGCACTGGTAGAATTCACTATCTCGTAT166180.10464479176171314No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT162750.10248489504885554No Hit
TGGTTAGCACTCTGGATCTCGTATGCCGTCT160730.10121288590600645No Hit
TCCCCAGTACCTCCACCAATCTCGTATGCCG159620.10051391058493592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)