FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007740249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007740249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12286052
Sequences flagged as poor quality0
Sequence length31
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA213944117.413575980306774Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT8770027.138192154811001No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA3749713.0520056402170526No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT3743733.0471383321509626No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT3087752.5132157995098834No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA2209681.7985273055982507No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT1913951.557823457038925No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1668791.3582801049515336No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1650121.3430840110395106No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT1498321.2195292678233822No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT1383761.1262853193198272No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA1167170.9499959791802933No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT1058220.8613181842303776No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1001200.8149078320684301No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT992240.8076150092804426No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA775910.6315372912307388Illumina Single End Adapter 1 (95% over 23bp)
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA770210.6268978838767735No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA617490.5025943240350929No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG509340.4145676739769618No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT504080.41028639631347813No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT480940.3914520303186084No Hit
TTTGGCAATGGTAGAACTCACACTGATCTCG455080.37040377169167116No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT445360.36249236125648826No Hit
TATTGCACTTGTCCCGGCCTGTAATATCTCG410190.33386640395140765No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG406350.33074090847084153No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT398060.3239934195297236No Hit
TTTGGCAATGGTAGAACTCACACATCTCGTA382180.3110681934277993No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTAT366330.2981673852593168No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT343690.27973998482181256No Hit
TATGGCACTGGTAGAATTCACTGATCTCGTA335730.2732610931485558No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT330530.26902865135195586No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA323770.26352647701637594No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT308610.25118728131705775No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGTA291900.23758649238990684No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT271030.22059975002547602No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTAT237840.19358537632756234No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT233990.19045174153584896No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT232850.1895238600650559No Hit
TTTGGCAATGGTAGAACTCACACTGGATCTC228240.18577163762614712No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT221000.17987877635549646No Hit
TTCCGGCTCGAAGGACCAATCTCGTATGCCG220710.17964273633222455No Hit
ATGGCACTGGTAGAATTCACTGATCTCGTAT220450.17943111424239455No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA217490.17702187814279152No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG212570.1730173370583162No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG211100.171820858319662No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA208600.1697860305328351Illumina Single End Adapter 1 (95% over 24bp)
CTAAGCCAGGGATTGTGGGTATCTCGTATGC202550.16486174728871406No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT202470.1647966327995356No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT194600.15839099492660458No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA193190.1572433520548342No Hit
CTCGTACGACTCTTAGCATCTCGTATGCCGT190040.15467946904343235No Hit
CTGGATGATGATAAGCAAATGCTGACTGAAC187870.15291323852446662No Hit
TCGTATCCCACTTCTGACACCAATCTCGTAT184340.15004006168946704No Hit
TATTGCACTTGTCCCGGCCTGTGAATCTCGT175160.14256817405623873No Hit
TAGCTTATCAGACTGATGTTGACTATCTCGT175070.14249492025591295No Hit
CAAAGTGCTTACAGTGCAGGTAGAATCTCGT171850.13987406206647993No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA171590.13966243997664993Illumina Paired End PCR Primer 2 (95% over 24bp)
CACCCGTAGAACCGACCTTGCGAATCTCGTA171100.1392636137304319No Hit
TTTCCGGCTCGAAGGACCAATCTCGTATGCC167010.1359346354711831No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA166260.13532418713513503No Hit
TCGTATCCGGCTCGAAGGACCAATCTCGTAT162870.13256496065619777No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG160460.13060338666969667No Hit
TATGGCACTGGTAGAATTCACTATCTCGTAT157620.12829182230386132No Hit
ATCCGGCTCGGAGGACCAATCTCGTATGCCG157190.1279418319245271No Hit
CTCGTACGACTCTTAGCGGATCTCGTATGCC156170.12711162218750174No Hit
GGCTGTAGGAATACTTTTATCTCGTATGCCG151900.12363613632760141No Hit
CTGTGCGTGTGACAGCGGCTGAATCTCGTAT150770.12271639416795567No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA149810.12193502029781414No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG145140.11813396199202152No Hit
TATTGCACTTGTCCCGGCCTGTAAAATCTCG140550.11439801817540736No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG137650.11203761794268817No Hit
TGAGAACTGAATTCCATAGGCTGATCTCGTA136980.11149228409581859No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT134490.109465595620139No Hit
TGGTGTAATGGTTAGCACTCTGGATCTCGTA133000.10825283825919019No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCG126180.10270182805672645No Hit
ATTGCACTTGTCCCGGCCTGTATCTCGTATG124960.10170883209675492No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT124730.10152162794036684No Hit
TGGTTAGTACTCTGCGATCTCGTATGCCGTC124090.10100071202693917No Hit
TAGCTTATCAGACTGATGTTGACAATCTCGT123580.10058560715842649No Hit
TCGTATCCCACTCCTGACACCAATCTCGTAT123060.10016236297876649No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)