FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12068186
Sequences flagged as poor quality0
Sequence length31
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA150738212.490543317777833Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT8884067.361553757955007No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT4821363.9950991806059335No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT3282662.7200939726981335No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA2382771.974422667996665No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT1879691.55755802901944No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA1635641.3553321103933929No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT1616461.339439083885515No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1520591.2599988100945743No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1429511.1845276498058614No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1393461.1546557204206167No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA990090.8204132750357014No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA978630.8109172331284917No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT977130.8096742957060822No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG939000.7780788264284293No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA684740.567392647080514No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA680080.5635312548215614No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC661170.5478619570497173No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG652990.5410838049728435No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT640530.5307591381173608No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT633520.5249504772299665No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA573650.4753407015768567Illumina Single End Adapter 1 (95% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCT570520.4727471054887619No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT483410.4005655862446933No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG477380.39556897780660655No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA455240.3772232214518404No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA448520.37165486179944524No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT427090.35389742915795297No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT423530.3509475243421008No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT396000.3281354795161427No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT392560.3252850096940833No Hit
ATAACCCAGAGGTCGATGGATATCTCGTATG368140.3050499884572545No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG329580.2730982104518442No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT305720.25332721918604834No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC296390.24559614841866045No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA295140.24456036723331906No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT289540.23992006752298978No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA265460.21996677876857385No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTAT255080.21136565180549918No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA252310.20907036069878274No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG242120.20062667247587998No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC236680.19611895275727437No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA233980.19388166539693702No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC230890.1913212143067732No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG230650.19112234431918765No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT216920.17974532377939817No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA213160.17662969397389136No Hit
GGTAGCGTGGCCGAGCGGTCTATCTCGTATG210270.17423496787338213No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC206740.17130992180597812No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT204350.16932950817960546No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG201100.16663647709771792No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT192730.15970088628067217No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT189550.15706585894516376No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT187120.15505230032086015No Hit
AAGCGTTGGATTGTTCACCCATCTCGTATGC177930.14743723704623046No Hit
TTCACAGTGGCTAAGTTCCGCATCTCGTATG170870.14158714491142246No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT169510.14046021498177108No Hit
TTGATCCTTCGATGTCGGCTCTATCTCGTAT166520.13798262638643455No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT164960.13668997146712852No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT161160.1335411966636908No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT156550.12972123565215188No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA155520.12886775195543057Illumina Paired End PCR Primer 2 (95% over 24bp)
TACAGTACTGTGATAACTGAATATCTCGTAT155490.1288428932069824No Hit
GGTAGCGTGGCCGAGCGGTCATCTCGTATGC152670.1265061708528523No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTAT143160.11862594759477522No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC139100.1152617303047865No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG135710.11245269173014072No Hit
ATTGCACTTGTCCCGGCCTGTATCTCGTATG128200.10622971836860982No Hit
ACGAGAACTTTGAAGGCCGAAATCTCGTATG125400.10390956851344518No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT125260.10379356102068694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)