FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16231183
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA176926310.900394629276251Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT9993756.157129766819831No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA5171193.1859600128961643No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA5146333.170643815672585No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT4624982.8494411035843785No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT4105932.5296554169834695No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA4046372.4929606178428276No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT3945042.4305314036567762No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT3559062.1927298829666326No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA3254322.0049801668800113No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA3043191.874903388126423No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG1974571.216528702806197No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT1781891.0978189328528918No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT1725671.063181901159022No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC1613450.9940433793396328No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT1509300.9298767686865461No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT1428950.8803732913368053No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG1221560.7526007192451715No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1188690.7323495767375674No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG1080520.6657062519718987No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT983870.6061603766034799No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA922720.5684859815824884No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT889080.5477604435856586No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC872720.5376810796847032No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT803750.4951887980068982No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA778870.47986027882256027No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT761610.46922642668744474No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG749300.46164225984021007No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT731900.4509221539797808No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC727900.44845776182795793No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA674780.41573063405175087No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA673690.41505908719037915Illumina Single End Adapter 1 (95% over 23bp)
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG651780.40156037917876963No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT630060.38817872979437174No Hit
AACATTCATTGCTGTCGGTGGATCTCGTATG616770.3799907868699404No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG600470.3699483888512624No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA595100.3666399423874403No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA589380.36311586161033366No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT580300.35752169142569584No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT543850.3350649179422104No Hit
TCTTTGGTTATCTAGCTGTATGATCTCGTAT541980.33391281461123323No Hit
AACATTCATTGCTGTCGGTGGGAATCTCGTA538760.33192897892901585No Hit
AACATTCATTGCTGTCGGTGGGATCTCGTAT513790.31654501092126186No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG512010.3154483564137007No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA493540.30406902565265886No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT478890.2950431893966078No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG477390.2941190423396742No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT468970.2889314968600871No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT447410.27564842316176213No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT421020.2593895959401111No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT411020.253228615560554No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA400580.24679655204429646No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG397830.24510228243991827No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT378980.23348883442445323No Hit
AACATTCATTGCTGTCGGTGGGTATCTCGTA375950.2316220573694474No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA374690.23084577384162325No Hit
AACATTCAACGCTGTCGGTGAATCTCGTATG369430.22760509816197624No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT364020.22427200777663586No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA349540.21535090818703725No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG345680.21297276976052823No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT316480.19498270705222165No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT315540.19440357489654328No Hit
ATCACATTGCCAGGGATTTCCATCTCGTATG315250.1942249064655361No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT308680.19017714235616714No Hit
CAACGGAATCCCAAAAGCAGCTATCTCGTAT296210.1824943998228595No Hit
AACATTCATTGCTGTCGGTGGAATCTCGTAT295050.1817797260988309No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA275520.16974733141755594No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA259160.15966796751660062No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC245730.1513937708668555No Hit
ACCACAGGGTAGAACCACGGACATCTCGTAT232010.14294090578610322No Hit
ACCACTGACCGTTGACTGTACCATCTCGTAT231580.14267598362978226No Hit
TACAGTACTGTGATAACTGAAAATCTCGTAT230140.14178880245512604No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG224560.13835097540333322No Hit
TGAGAACTGAATTCCATAGGCTATCTCGTAT224100.13806757030587358No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT223550.13772871638499795No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA218870.13484537756736523Illumina Paired End PCR Primer 2 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTGAATCTCGT217780.13417383070599354No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA211970.13059430110547088No Hit
TGAGAACTGAATTCCATAGGCTGATCTCGTA203490.1253697897436065No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT200880.1237617738645421No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC196600.12112487426209169No Hit
ACTGCAGTGAAGGCACTTGTAGAATCTCGTA194840.12004054171528963No Hit
TTCACAGTGGCTAAGTTCCGCATCTCGTATG190100.11712023701537959No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTATG188740.11628234368375984No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT186460.11487764015722082No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTAT175500.1081252056612263No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGTA171930.10592573566572441No Hit
TGAGGTAGTAGGTTGTATAGTAATCTCGTAT171400.10559920370560791No Hit
CAACGGAATCCCAAAAGCAGCTGATCTCGTA169830.10463192978601744No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT166230.10241397684937692No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG165810.10215521567343551No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA164210.10116945881270638Illumina Single End Adapter 1 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)