FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9929105
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA114480511.529790449390957Illumina Single End Adapter 1 (95% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTAT4835424.86994547846961No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA4703244.736821697423887No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT2982093.0033824800926165No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA2879872.9004326170384944No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT2626262.6450118112357557No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT2251682.2677572651311473No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA2015612.030001697031102No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT1734071.7464514676801182No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1689281.701341661710698No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1342191.3517733975015875No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1085381.0931297433152334No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA918270.9248265578821052No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA856550.8626658696831185No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG751860.7572283705328929No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC716960.7220791803490848No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT694920.6998818121069321No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT595330.599580727568094No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT587650.5918458914474165No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT541680.545547660136538No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT519490.5231992208764032No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA495580.4991185006100751Illumina Single End Adapter 1 (95% over 23bp)
TGAGGTAGTAGGTTGTATAGTATCTCGTATG477430.48083890743425517No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT472760.4761355630744161No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG465460.46878344019929286No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG464190.46750437224704544No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC446880.45007077677192453No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT423410.4264331981583436No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA372770.375431622487626No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT362370.3649573652408752No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT340170.34259885457954165No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA327140.3294758188175067No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT326280.32860967831441No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT305770.3079532344556735No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA305390.30757052121011913No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG288930.29099299483689617No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT277350.27933031224868704No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC273130.27508018094279396No Hit
TGTCAGTTTGTCAAATACCCCAGATCTCGTA273000.27494925272720955No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT267310.269218625445093No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGTA265360.2672547022113272No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG240150.24186469978915523No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA230870.23251843947666986No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG209890.21138863976158978No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC202990.20443937293441855No Hit
AATGACACGATCACTCCCGTTGAATCTCGTA196970.1983763894127416No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA196700.19810446158037404No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG191850.19321983199895662No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG187900.18924162852543105No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT187420.1887582012678887No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTAT184770.186089279950207No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT184270.18558570989026704No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA182610.18391385729126644No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT181280.18257436093182616No Hit
TCGTATCCCACTTCTGACACCAATCTCGTAT178200.1794723693625961No Hit
TAGCTTATCAGACTGATGTTGACAATCTCGT174370.17561502270345614No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA173040.17427552634401589Illumina Paired End PCR Primer 2 (95% over 24bp)
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT169710.17092174974481586No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC166040.1672255455048567No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT164690.16586590634301882No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA161300.162451701336626No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG161180.16233084452224042No Hit
TGTAAACATCCTTGACTGGAAGCTATCTCGT160720.16186756006709568No Hit
ACCACAGGGTAGAACCACGGACATCTCGTAT160130.16127334739636656No Hit
GTCAGTTTGTCAAATACCCCAAATCTCGTAT157790.15891663951584759No Hit
TTCACCACCTTCTCCACCCAGCATCTCGTAT149710.1507789473472181No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA146120.14716331431684931No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTAT146020.14706260030486132No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT144470.14550153311904748No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT143180.14420232236440242No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG140640.1416441864599075No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTATG138760.13975076303453332No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT136850.13782712540556274No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA135780.13674948547729127No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA130230.13115985781195785No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA129310.13023328890166838No Hit
TACCACAGGGTAGAACCACGGACATCTCGTA129220.13014264629087918No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT128760.12967936183573445No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG128640.12955850502134886No Hit
CTACAGTCCGACGATCTGGTCAAATCTCGTA128240.1291556489733969No Hit
TCTACAGTCCGACGATCTGGTCAAATCTCGT128030.12894414954822211No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT126340.12724208274562512No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT124410.12529830231425693No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA120830.12169274068508693No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT120480.12134024164312895No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG118010.11885260554702563No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT116720.11755339479238057No Hit
GTAAACATCCTTGACTGGAAGCTATCTCGTA111340.11213498094742677No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA108600.10937541701895588Illumina Single End Adapter 1 (95% over 24bp)
TAGCTTATCAGACTGATGTTGACTATCTCGT106210.10696835213244295No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG104750.10549792755741833No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTAT104720.10546771335382193No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT103250.10398721737759847No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA102050.10277864923374262No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA101540.10226500777260386No Hit
TCGTATCCCACTCCTGACACCAATCTCGTAT101330.1020535083474291No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)