FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4832276
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA4705139.736881750959588Illumina Single End Adapter 1 (95% over 24bp)
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT3173236.566739979256152No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT1766523.6556686745541853No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA1693603.504766697928678No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT1692793.503090469170221No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1672503.4611019734799915No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA997182.063582460935592No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT961511.989766313016889No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG781501.6172503391776463No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG711961.47334299613681No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT698451.4453851559803288No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA634271.3125698946003912No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT574971.1898533941356No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT572721.1851972031398867No Hit
GGATATCATCATATACTGTAAGATCTCGTAT533731.1045105867297316No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC451730.9348182926637468No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT424750.8789853890795972No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT416490.8618919945797798No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC407810.8439294444274292No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT402080.8320716780250135No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT376900.7799637272374342No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG366280.7579865057376689No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA356090.7368991340726398No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT345270.7145080289288112No Hit
TCCTGTACTGAGCTGCCCCGAATCTCGTATG313870.649528296810861No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG309140.6397399486287622No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG305820.63286948013731No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT300790.6224603064891161No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA282580.5847762006971455No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT271390.5616194108117997No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT249870.5170855307105803No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT242770.5023926613463304No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG233940.4841196984609323No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG224480.4645430020967346No Hit
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA223540.4625977489696367No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC211500.43768195359702133No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT210110.4348054622707809No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT203170.4204436998217817No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT196720.40709595230073775No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA190460.3941413942415541No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC183080.37886908777561545No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT182000.3766341160976732No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA175750.3637002522206927Illumina Single End Adapter 1 (95% over 23bp)
TGAGATGAAGCACTGTAGCTATCTCGTATGC171710.3553398026106125No Hit
AAACCGTTACCATTACTGAGATCTCGTATGC166460.3444753569539488No Hit
TTTGGCAATGGTAGAACTCAATCTCGTATGC165580.34265426892006995No Hit
TATGGCACTGGTAGAATTCACTATCTCGTAT157060.32502282568297014No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG150990.3124614570856466No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT145090.300251889585777No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG139560.2888080068274246No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT138180.28595220968338725No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT133910.2771157938826342No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA132100.27337014690386063No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG131640.27241821452251486No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA130940.27096962176829303No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG126480.26174001650567974No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA121830.2521172217812062No Hit
TCCTGTACTGAGCTGCCCCGAGTATCTCGTA113770.23543771092545213No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT113360.23458924945512216No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA97780.2023477135825851No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA94820.19622223565044714No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATGC92530.19148326792592144No Hit
TATGGCACTGGTAGAATTCAATCTCGTATGC85710.1773698356633603No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG84020.17387251887102476No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG77920.16124906772709174No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT76990.15932450878219706No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA74840.15487525960851573Illumina Paired End PCR Primer 2 (95% over 24bp)
AACATTCAACGCTGTCGGTGAGTATCTCGTA73350.15179182646024358No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT68580.14192070154933203No Hit
TCGTTTCCGGCTCGAAGGACCATCTCGTATG66030.13664368508752398No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA65770.1361056363502416No Hit
TGAGGTAGTAGATTGTATAGATCTCGTATGC65590.13573314107058454No Hit
ACCACAGGGTAGAACCACGGACATCTCGTAT65280.1350916214222863No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA62470.12927655622319587No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT62470.12927655622319587No Hit
ATGGCACTGGTAGAATTCACTATCTCGTATG62140.1285936482104913No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT60420.12503424887154624No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA59590.12331663174868324No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATGC58650.12137137862158537No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGTA58540.1211437426173505No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTATG58490.12104027170633466No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT57580.1191571011258463No Hit
TGGAGAGAAAGGCAGTTCCTGAATCTCGTAT57390.11876391166398609No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTAT56570.11706698872332624No Hit
TCGTCTGATCTCGGAAGCTAAGCAGGGTCGG55980.11584603197333929No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT55680.11522520650724422No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT55600.11505965304961885No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT55120.11406633230386676No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT54410.11259704536744176No Hit
TATGGCACTGGTAGAATTCACTGATCTCGTA54130.11201760826575304No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT53350.11040346205390586No Hit
ATGGCACTGGTAGAATTCACTGATCTCGTAT52390.10841682056240165No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG52100.10781668927850975No Hit
TACCACAGGGTAGAACCACGGAATCTCGTAT51750.10709239290139884No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA51570.1067198976217418No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT48600.10057372550740064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)