FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14807028
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA167969311.3438902121344Illumina Single End Adapter 1 (95% over 24bp)
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT9763556.593862049832013No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT7629625.1527018115991945No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT3875732.6174935307747105No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA3667892.4771277531183165No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG2820611.9049129913173664No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT2632141.7776288394943267No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA2620251.7695988688614623No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT2257271.5244585206430352No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC1905031.2865714848381458No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT1686891.1392495509564782No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1420720.9594903177058893No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT1419250.9584975458950978No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG1383330.934238795253173No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1342630.9067518478387425No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG1253630.8466452552125923No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG1165130.7868763400731058No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG1157390.7816490925795507No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG1109980.7496305132940925No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA1058190.7146538792254598No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT958500.6473277419344382No Hit
GGATATCATCATATACTGTAAGATCTCGTAT926750.625885221531289No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT895420.6047263502169375No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA852180.5755240011702552No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG846810.5718973449634863No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT817500.5521026906952563No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT809960.5470105141963668No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT800470.5406013954994885No Hit
TCCTGTACTGAGCTGCCCCGAATCTCGTATG769780.5198747513680666No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT708400.4784214631052227No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATGC691400.4669404285586547No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT681160.4600247936317808No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG675970.45651970132021097No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA675550.45623605223141334Illumina Single End Adapter 1 (95% over 23bp)
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT625060.4221373796281063No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC611020.4126553958025878No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC605270.40877210470595454No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC576810.38955150216505297No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT554240.3743087404170506No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT540950.3653332728215277No Hit
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA528660.35703316019933234No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT527290.35610792388587365No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT507230.3425603031209234No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT505460.34136492481813363No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG470430.31770724010247026No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG467560.31576897132902026No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG458060.3093530990824087No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT448700.303031776532063No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA438830.2963660229453203No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG425870.28761342249099553No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA425490.28735678760113104No Hit
TCCTGTACTGAGCTGCCCCGAGTATCTCGTA402370.27174258061779855No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTAT361720.24428940095203439No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA292390.1974670406512367No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA279370.18867391889851223No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC277310.18728268765345754No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA275290.1859184706073359No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT274740.1855470253720058No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA261180.17638921193368445No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT250280.16902784272441437No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT245890.16606303439150652No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA239540.16177453031087669No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA227560.1536837777304129Illumina Paired End PCR Primer 2 (95% over 24bp)
CGCGACCTCAGATCAGACGTGGCATCTCGTA223740.1511039217323017No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA216910.14649124726447468No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTATG216120.14595771683554593No Hit
TAGCACCATTTGAAATCGGTTATCTCGTATG211720.14298615495290481No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT211600.1429051123561055No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATGC204510.13811684559521328No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT200020.13508450176497266No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT199330.1346185068333767No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT197690.13351092467711953No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT192440.12996531106715No Hit
AAACCGTTACCATTACTGAGATCTCGTATGC191390.1292561883451561No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG188840.12753403316317088No Hit
TGTAAACATCCTTGACTGGAAGCTATCTCGT187040.12631839421118135No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT185650.12537965079825608No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT182800.12345488912427262No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG180270.12174624104175395No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC179510.12123297126202504No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGTA178640.12064541243523008No Hit
TGGAGAGAAAGGCAGTTCCTGAATCTCGTAT175310.11839648037404941No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT172300.11636366190433353No Hit
TCTCCCAACCCTTGTACCAGTGAATCTCGTA170730.11530335459620931No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT169080.11418901889021889No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT168930.11408771564421975No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT167480.11310845093289484No Hit
TACAGTACTGTGATAACTGAAAATCTCGTAT166820.11266271665049868No Hit
ACCACAGGGTAGAACCACGGACATCTCGTAT166290.11230477851463508No Hit
GTAAACATCCTTGACTGGAAGCTATCTCGTA164680.11121745700757775No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA163800.11062314463104954No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA155920.1053013474412286Illumina Single End Adapter 1 (95% over 24bp)
TAGCTTATCAGACTGATGTTGAAATCTCGTA153360.1035724387095101No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTAT153250.10349814966244407No Hit
TGAGAACTGAATTCCATAGGCTATCTCGTAT149300.10083049751780032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)