FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15858536
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA190939112.040146707110921Illumina Single End Adapter 1 (95% over 24bp)
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT6579724.149008458283917No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT6531824.118803904723614No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT4869553.0706176156487586No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT4532072.8578110867232636No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA4383052.7638427658139437No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA3871402.441208948921893No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC2153111.3576978354117932No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT2045141.2896146277310843No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1996191.2587479701783317No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1805051.1382198205433338No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG1724361.087338705161687No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT1702421.0735038845956524No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG1601091.0096076964481462No Hit
GGATATCATCATATACTGTAAGATCTCGTAT1597951.0076276902231076No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT1577020.994429750640286No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG1565330.9870583261910179No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA1547520.975827781328617No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG1498660.9450178755466456No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT1203160.7586828948145025No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA1196120.7542436451889379No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT1107960.698652132832438No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT1054900.6651938110806698No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT1051570.6630939955617593No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT1035230.6527903962887873No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC958250.6042487150137945No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT899070.5669312728488936No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA775940.48928854466767935No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG715610.4512459409872387No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA688770.43432130178977424Illumina Single End Adapter 1 (95% over 23bp)
AAACCGTTACCATTACTGAGATCTCGTATGC655300.41321594881141616No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA647770.4084677173227087No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG620120.3910323121882121No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG613550.38688943292117256No Hit
TCCTGTACTGAGCTGCCCCGAGTATCTCGTA606750.3826015213510251No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT598560.377437110209921No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG590740.3725060119042514No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT576590.3635833723869593No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA575210.3627131785683117No Hit
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA542320.3419735592238779No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG521000.3285296953010038No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA516800.32588127933120686No Hit
TCCTGTACTGAGCTGCCCCGAATCTCGTATG507560.32005476419765355No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT479690.302480632512358No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT475610.29990788557026954No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT471140.2970892142881285No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA465050.29324901113192287No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC444670.2803978879260986No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC399880.25215442333390675No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT387520.24436051347993282No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT371070.2339875509315614No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT360230.22715211542856162No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT356800.2249892423865608No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT354880.22377853794322503No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT342300.21584590153845223No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT332660.20976715631253728No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT322720.2034992385173512No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG289900.18280375943908064No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT286870.1808931164894414No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA276080.17408920974798683No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT274120.1728532822954149No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG273440.17242449113840017No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG271840.1714155707689537No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT266690.16816810832979792No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA247490.15606106389644037No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT245300.15468010414076055No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT228950.14437019911547952No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA216370.13643756271070673Illumina Paired End PCR Primer 2 (95% over 24bp)
CCCCCACTGCTAAATTTGACTGGATCTCGTA204840.12916703029838317No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA202910.12795002010273837No Hit
GTACAGTACTGTGATAACTGAAATCTCGTAT202510.12769779001037673No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATGC200290.1262979129977698No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA196730.12405306517575142No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT195040.12298739303552358No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT191890.12100108105817586No Hit
TACAGTACTGTGATAACTGAAAATCTCGTAT191880.12099477530586682No Hit
ACCACAGGGTAGAACCACGGACATCTCGTAT190840.12033897706572663No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA190580.12017502750569158Illumina Single End Adapter 1 (95% over 24bp)
TAGCAGCACGTAAATATTGGCGATCTCGTAT189180.11929222218242591No Hit
TGAGGTAGTAGATTGTATAGATCTCGTATGC187330.11812565800525346No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCG178900.11280990880873241No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG176270.11115149595145479No Hit
CTTCTCACTACTGCACTTGACTAGATCTCGT176220.11111996718990959No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT172820.10897601140483586No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG166000.10467548833007032No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTAT162280.1023297484711073No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT162230.10229821970956209No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT159890.10082267366924665No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)