FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10001578
Sequences flagged as poor quality0
Sequence length31
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA120678012.065896001610946Illumina Single End Adapter 1 (95% over 24bp)
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT5626615.62572226102721No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT3264353.263834966842232No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT2724792.724360095976855No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA2663982.663559690280874No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1817811.8175231948398545No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT1772051.7717704146285718No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1448141.447911519562213No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1428541.4283146119542336No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT1342621.342408167991091No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1060171.0600027315689584No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC1027481.0273178892370785No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC746770.7466521782862664No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG687000.686891608504178No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT665770.6656649580696167No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA642450.6423486373850207No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG620030.6199321747028319No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG615950.6158528184252524No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT572650.5725596500872163No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG552140.5520528860545806No Hit
TCCTGTACTGAGCTGCCCCGAGTATCTCGTA534170.5340857212731831No Hit
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA500240.5001610745824309No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA487960.4878830120606968No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT483970.4838936415833581No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA481360.48128405337637725Illumina Single End Adapter 1 (95% over 23bp)
GGATATCATCATATACTGTAAGATCTCGTAT447570.4474993845971106No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT439620.43955063890918017No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT431890.431821858510727No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG431440.43137192950952336No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT416370.4163043071803269No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA401820.4017566028080769No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT399310.3992469988235856No Hit
CGCGACCTCAGATCAGACGTATCTCGTATGC388510.388448702794699No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT387670.3876088353257856No Hit
TCCTGTACTGAGCTGCCCCGAATCTCGTATG382290.38222968415584024No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT371520.3714613833937005No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG347540.34748516684067254No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA340720.34066624286687563No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT327510.3274583270759874No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT319510.3194595892768121No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA279910.2798658371708944No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG265260.26521814857615467No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA226500.2264642639391504No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA226280.2262442986496731No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG206630.20659739893044876No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC205130.2050976355931034No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT198620.19858866270902453No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG195550.195519147078591No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT192820.19278957780462241No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT189030.18900017577226313No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT188690.1886602294157982No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG187390.1873604345234322No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC185710.1856806995856054No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG185150.1851207879396631No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT176960.1769320801177574No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT171040.1710130141463677No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA163750.16372416432686923No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG160000.1599747559835058No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT159280.15925486958158003No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG158880.15885493269162126No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA155510.15548546439371866No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG155430.1554054770157269No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCG151140.15111615387091917No Hit
CGCGACCTCAGATCAGACGATCTCGTATGCC148780.14875652622016244No Hit
TGGAGAGAAAGGCAGTTCCTGAATCTCGTAT148700.1486765388421707No Hit
TTATGGTTTGCCTGGGACTGAATCTCGTATG145840.14581699007896554No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT144910.1448871368098114No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT143990.14396728196290626No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTAT141150.14112773004419904No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT139610.13958797301785777No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT137650.13762828225705984No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA137000.13697838481087685No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT136730.13670842741015468No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG135530.1355086167402784No Hit
CTTCTCACTACTGCACTTGACTAGATCTCGT130240.13021945137057372No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT129370.12934958863491341No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTATCT125640.12562017713604795No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT124870.12485029862287732No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA122380.122360691482884Illumina Single End Adapter 1 (95% over 24bp)
TACCCTGTAGATCCGAATTTGTGATCTCGTA122150.1221307277711577No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG121190.12117087923525667No Hit
ACCACAGGGTAGAACCACGGACATCTCGTAT119800.11978109854264996No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA115920.11590171071004995No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT115900.11588171386555202No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT115650.11563175330932779No Hit
TCTACAGTCCGACGATCTGGTCAAATCTCGT115340.11532180221960975No Hit
CTACAGTCCGACGATCTGGTCAAATCTCGTA115050.11503184797438964No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG114330.11431196157246387No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT113640.113622070437285No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT112830.1128121982351185No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA105600.10558333894911383Illumina Paired End PCR Primer 2 (95% over 24bp)
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA105010.10499343203642467No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT104050.10403358350052362No Hit
GTACAGTACTGTGATAACTGAAATCTCGTAT102800.10278378071940247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)