FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1509655
Sequences flagged as poor quality0
Sequence length31
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGAATCTCGTAT32290921.389589012059044No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1265388.381915073311452Illumina Single End Adapter 1 (95% over 24bp)
TAGCTTATCAGACTGATGTTGACATCTCGTA926806.13915099807572No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA582403.8578350682771894No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT536273.5522685646720604No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT518803.4365467606837328No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT349852.317416893263693No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT342742.2703200400091412No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT307052.033908409537278No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT292381.9367338895310517No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT203771.3497785917974636No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT168281.1146917673243224No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA154341.0223527892134294No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG129170.855625954274321No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT125730.83283929109631No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT106470.7052604734194237No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT95420.6320649419900572No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG77760.5150845723029434No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT70970.47010740864634637No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG70670.46812019964826396No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT70140.46460946375165185No Hit
TAGCTTATCAGACTGATGTTGACTATCTCGT68680.4549383799609844No Hit
TTTGGCAATGGTAGAACTCACACATCTCGTA68490.45367981426219894No Hit
CGCGACCTCAGATCAGACGTATCTCGTATGC67130.4446711334708924No Hit
TATGGCACTGGTAGAATTCACTATCTCGTAT58690.3887643203248424No Hit
TGAGAACTGAATTCCATAGGCTGATCTCGTA57710.3822727709311068No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG57600.3815441276318099No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG56680.37545002003769073No Hit
TAGCTTATCAGACTGATGTTGACAATCTCGT56240.37253544684050327No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG50460.3342485534774502No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGTA49580.32841940708307527No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA44330.29364324961663424No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTAT43730.2896688316204696No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT42960.28456832852539155No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTAT41550.27522844623440457No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT41390.2741686014354273No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA41040.27185019093766455Illumina Single End Adapter 1 (95% over 23bp)
AGCAGCATTGTACAGGGCTATGATCTCGTAT40960.2713202685381759No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT40000.2649611997443124No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT38430.2545614726543482No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC37980.25158065915722466No Hit
TATGGCACTGGTAGAATTCACTGATCTCGTA35430.23468938267352477No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT34200.22654182578138712No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG33350.22091140028682052No Hit
TACCCTGTAGATCCGAATTTGTGATCTCGTA32670.21640705989116718No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA32600.21594337779161465No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA31850.2109753552964088No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT31580.20918686719813467No Hit
TGAGAACTGAATTCCATAGGCTATCTCGTAT31480.2085244641987739No Hit
CTTATCAGATTGTATTGTAATTATCTCGTAT30560.20243035660465472No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA30050.1990521013079147No Hit
TAGCTTATCAGTCTGATGTTGAATCTCGTAT29190.193355435513412No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA25470.16871404393719094No Hit
TTTGGCAATGGTAGAACTCACACTGATCTCG24850.1646071453411541No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT23650.15665830934882474No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT22930.15188900775342712No Hit
ATGGCACTGGTAGAATTCACTGATCTCGTAT22790.150961643554322No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA22410.14844451215675103No Hit
CTAGACTGAAGCTCCTTGAGGATCTCGTATG22290.1476496285575181No Hit
AAGTTTCTCTGAACGTGTAGAGCATCTCGTA22150.146722264358413No Hit
TAGCACCATTTGAAATCGGTTATCTCGTATG21810.14447009416058634No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA21330.1412905597636546Illumina Paired End PCR Primer 2 (95% over 24bp)
ACCCTGTAGATCCGAATTTGTGTATCTCGTA21300.14109183886384638No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGTA20580.13632253726844876No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA19580.12969850727484095No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA19550.1294997863750327No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT19520.12930106547522446No Hit
ACCACAGGGTAGAACCACGGACATCTCGTAT18960.1255916086788041No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA18450.12221335338206411No Hit
TACCACAGGGTAGAACCACGGACATCTCGTA18340.12148471008276726No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG18300.12121974888302295No Hit
TAGCACCATTTGAAATCGGTTAATCTCGTAT18140.12015990408404569No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT18020.11936502048481275No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT17520.11605300548800884No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGTA17370.11505940098896768No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA17280.11446323828954298No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT17070.11307219199088533No Hit
TTCACAGTGGCTAAGTTCCGCATCTCGTATG16860.1116811456922277No Hit
TAGCTTATTAGACTGATGTTGAATCTCGTAT16820.11141618449248339No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT16730.11082002179305867No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA16010.10605072019766106No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG15980.10585199929785281No Hit
TTTGGCAATGGTAGAACTCACATCTCGTATG15760.10439471269925911No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG15600.10333486790028185No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT15460.10240750370117675No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG15320.10148013950207167No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTAT15150.10035405440315832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)