FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14030056
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA156467911.152336099014857Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT13587409.684494488118936No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT10105547.202779518485173No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT5459573.891338708840506No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA3829612.729575705186066No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA3461162.4669609301630726No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT2379681.6961300795948355No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA2114291.5069718894921018No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT2059091.4676277842369267No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA1821401.2982129223147791No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT1665511.1871014627454088No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1560441.112212239209879No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT1490051.062041377454231No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA1431611.0203879442819044No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG1261430.8990912081890479No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG1249190.8903670805020308No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT853630.6084295030611425No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT807870.5758138100090263No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG797270.5682586013911848No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT791670.5642671704232685No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA658030.4690145213960657No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA649220.4627351451768974No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA573750.40894348532892527No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT567310.40435333971582155No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC561300.40006967898061135No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT556420.3965914319942843No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA550850.3926213836922675Illumina Single End Adapter 1 (95% over 23bp)
TGTCAGTTTGTCAAATACCCCAATCTCGTAT524840.37408261235735624No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTAT523580.37318454038957505No Hit
TACCCTGTAGATCCGAATTTGTGATCTCGTA476420.3395709895954799No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA464610.3311533467863564No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT449440.3203408453964831No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT428470.30539436193269653No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG418220.29808861775034967No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG407460.2904193682477105No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA405820.289250449178535No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA392180.2795284637495388No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT386460.2754515021180243No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA364060.2594857782463591No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT356550.2541329842161713No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT351560.25057633412154595No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGTA349760.24929337416757283No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTAT340880.2429641050613055No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT337620.24064052203355427No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT336000.23948585807497846No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT315280.22471756349368815No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG300890.2144610114172032No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG295170.2103840497856887No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA294270.20974256980870212No Hit
TACCCTGTAGATCCGAATTTGTGTATCTCGT289090.20605049616337956No Hit
TGAGAACTGAATTCCATAGGCTGATCTCGTA278240.1983170986630417No Hit
TTAATGCTAATCGTGATAGGGGTATCTCGTA266320.18982105274561983No Hit
CTAAGCCAGGGATTGTGGGTATCTCGTATGC266200.1897355220820216No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC260140.18541622357031218No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA238120.16972134680004128No Hit
TGGTGTAATGGTTAGCACTCTGGATCTCGTA236570.16861657572856445No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG235780.16805349885987625No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT235670.1679750957515779No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG232950.16603640071001854No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA231750.16518109407403647No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT223080.15900150362906607No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT220270.15699866058980805No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT215960.15392668425557246No Hit
CTTCTCACTACTGCACTTGACTAGATCTCGT211560.1507905599236382No Hit
ATCACATTGCCAGGGATTTCCATCTCGTATG209360.1492224977576711No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT207230.14770432847880294No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT206400.14711274138891534No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT203470.14502436768605914No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT199750.14237291711451472No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA198450.14144633492553416No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC194920.1389303079046869No Hit
TTAATGCTAATCGTGATAGGGGATCTCGTAT190160.13553759158195805No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA187600.1337129374251963No Hit
ATCACATTGCCAGGGATTTCCAATCTCGTAT186540.13295741656341215No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT184340.13138935439744504No Hit
TAGCAGCACATCATGGTTTACAATCTCGTAT177640.12661389234654516No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG169160.1205697254522719No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA169040.12048419478867368No Hit
TTAATGCTAATCGTGATAGGGGTTATCTCGT166360.11857400996831374No Hit
ATTGCACTTGTCCCGGCCTGTATCTCGTATG163180.11630744738296128No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCG162870.1160864931686659No Hit
TAAAGTGCTTATAGTGCAGGTAGAATCTCGT159220.11348493548422045No Hit
TGTGCAAATCCATGCAAAACTGAATCTCGTA158090.11267952173533735No Hit
TTCACAGTGGCTAAGTTCCGCATCTCGTATG156320.11141794444726379No Hit
CTCCCACTGCTTCACTTGACTAGATCTCGTA155300.11069093380667903No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT154650.11022764271218874No Hit
CAACGGAATCCCAAAAGCAGCTGATCTCGTA154080.10982137206009726No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA151830.1082176721176309No Hit
CAAAGTGCTTACAGTGCAGGTAGAATCTCGT151670.10810363123283327No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA148300.10570164509678365Illumina Paired End PCR Primer 2 (95% over 24bp)
TGGCTCAGTTCAGCAGGAACAGATCTCGTAT145050.10338518962433221No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGTA144950.10331391407133372No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT143370.10218776033395732No Hit
TCGTATCCGGCTCGAAGGACCAATCTCGTAT141680.10098320348828257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)