FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11292288
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA114970410.181320207206902Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT7784516.893651667403453No Hit
TGAGGTAGTAGGATGTGTGGTTATCTCGTAT7197776.374058118248489No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA6659785.897635625304633No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT4365343.8657710465762123No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT4350813.852903857924984No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA3617053.2031152588385985No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA2776262.4585451593158094No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1932271.7111412673853164No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA1800001.5940082293331521No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1787251.5827173377087087No Hit
TCTTTGGTTATCTAGCTGTATGATCTCGTAT1476771.3077686293512882No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1334921.1821519252785617No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT1334551.181824268031421No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA1190621.0543655988936873No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT1015190.8990117857426236No Hit
TGAGGTAGTAGGATGTGTGGTATCTCGTATG996370.8823455441448181No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA994140.8803707450606999No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG859040.7607315718479727No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT693770.6143750495913671No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTAT604060.5349314505616576No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT584730.5178135732988743No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG584220.5173619376338967No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT580100.5137134299089786No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA558480.4945676199544326No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT557060.4933101245735143No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT513350.4546022914045409No Hit
TGAGGTAGTAGGATGTGTGGTTTATCTCGTA505600.4477392004171342No Hit
TGAGGTAGTAGGATGTGTGGTTAATCTCGTA464090.410979599528457No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT463410.41037741864182No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT429410.3802683743099715No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT427880.37891346731503833No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA394050.34895496820484917Illumina Single End Adapter 1 (95% over 23bp)
GTACAGTACTGTGATAACTGAATCTCGTATG344940.3054651103478764No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG334220.29597190578207005No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTAT303240.2685372530349917No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA297370.2633390150871108No Hit
ACCACAGGGTAGAACCACGGACATCTCGTAT265250.2348948237947881No Hit
CAACGGAATCCCAAAAGCAGCTGATCTCGTA244140.21620064950521986No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT241040.21345541311025718No Hit
CTCGGCGTGGCGTCGGTCGTGGATCTCGTAT227860.2017837306310289No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT227790.2017217414221104No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT220230.19502690685891116No Hit
CAGCATTGTACAGGGCTATGAATCTCGTATG216620.19183003479897076No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGTA211870.18762362419378606No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA202870.1796535830471203No Hit
TTAATGCTAATCGTGATAGGGGTATCTCGTA185500.16427140363405537No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT176340.15615967286700447No Hit
TAGCAGCACATAATGGTTTGTGGAATCTCGT173080.15327274685165662No Hit
TGTAAACATCCCCGACTGGAAGCTATCTCGT172700.15293623400324186No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT170950.15138650378027907No Hit
TTCACAGTGGCTAAGTTCCGCATCTCGTATG169430.15004045238661998No Hit
TTAATGCTAATCGTGATAGGGGTTATCTCGT167460.14829589893562756No Hit
TGTAGTGTTTCCTACTTTATGGAATCTCGTA165780.1468081579215833No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT158420.1402904353838655No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC158260.14014874576348035No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT156730.13879383876854717No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA150220.1330288423391256No Hit
CTAAGCCAGGGATTGTGGGTATCTCGTATGC149310.13222298262318494No Hit
ATCACATTGCCAGGGATTTCCAATCTCGTAT148180.1312222996792147No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA144330.12781289318869657No Hit
ACTGGACTTGGAGTCAGAAGGATCTCGTATG143270.12687419945364484No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA132310.11716846045726073No Hit
ATTGCACTTGTCCCGGCCTGTATCTCGTATG130650.11569843064576461No Hit
TACCACAGGGTAGAACCACGGACATCTCGTA130330.11541505140499428No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT128440.11374134276419448No Hit
TGGTGTAATGGTTAGCACTCTGGATCTCGTA126120.1116868432686095No Hit
CAACGGAATCCCAAAAGCAGCTATCTCGTAT124510.11026109146348374No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT123110.10902130728511353No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG122570.10854310481631357No Hit
TAGCAGCACATCATGGTTTACAATCTCGTAT122040.1080737579487877No Hit
TGAGGTAGTAGGATGTGTGGATCTCGTATGC120800.10697566339080264No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA119060.10543478876911393No Hit
TTAATGCTAATCGTGATAGGGGATCTCGTAT118670.10508942031942509No Hit
ATCACATTGCCAGGGATTTCCATCTCGTATG116350.10303492082384012No Hit
TGTGCAAATCCATGCAAAACTGAATCTCGTA114990.10183055905056619No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTAT114770.10163573582253659No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG114040.10098927692952925No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG113710.10069704208748485No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG113010.10007714999829972No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)