FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17301993
Sequences flagged as poor quality0
Sequence length31
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA192140111.105084830400752Illumina Single End Adapter 1 (95% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTAT12036146.9565049529265215No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT8136144.702429367530088No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT6250073.6123410753894074No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA5612263.2437072422812796No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT4102232.3709580740207215No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA4083402.360074934719948No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA3729792.15569963529635No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA3437911.9870023066128857No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT3402201.9663630658040376No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT3002581.7353954541537497No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT2503321.4468391011370771No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA2375921.3732059653474602No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA2232721.290440933596494No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT2197231.2699288457693862No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG1933051.1172412334232247No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1910761.104358324500536No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC1782881.0304477640234855No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT1715550.9915331719299622No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG1408510.8140738468683925No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG1153460.666663083264454No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG886230.5122126682168927No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT835650.4829790417785974No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA832990.4814416466357373No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT791500.4574617502157121No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA785310.4538841276840188No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT772610.4465439328290099No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG729540.42165084681284987No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA706810.40851363192668033Illumina Single End Adapter 1 (95% over 23bp)
TGAGGTAGTAGATTGTATAGTATCTCGTATG692280.4001157554508316No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT685220.3960353006731652No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT660210.38158031852168706No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT659870.3813838093680884No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT655880.3790777166537982No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC652150.37692189564520107No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT648440.3747776339985804No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT623620.36043246578587795No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA554370.3204081749426208No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA520760.30098266714129407No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT485410.2805514948480213No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG479490.2771299237030092No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT459870.2657901896041687No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT427110.24685595468683869No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA415880.2403653729370946No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT402130.2324183115783251No Hit
GGATATCATCATATACTGTAAGATCTCGTAT401640.23213510720990352No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT395810.22876555319378528No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG388430.2245001486244966No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA386840.22358117934737345No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT377770.2183390086910797No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA377550.21821185570933938No Hit
TCCTGTACTGAGCTGCCCCGAATCTCGTATG376470.21758765016261422No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG364250.21052487999503872No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT362730.20964636848483292No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT361630.20901060357613135No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGTA357220.20646176426033694No Hit
TACCCTGTAGATCCGAATTTGTGATCTCGTA338650.19572889666525703No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG336390.1944226887619247No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT335860.19411636566955032No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT330180.190833506868255No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTAT313440.18115832089401493No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA311940.18029136874578552No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG307010.17744198601860492No Hit
AACATTCATTGCTGTCGGTGGGAATCTCGTA305510.17657503387037551No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC297850.1721477982334174No Hit
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA294500.17021160510237174No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT283440.16381927792942697No Hit
AACATTCAACGCTGTCGGTGAATCTCGTATG283070.16360542973286374No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA278930.16121264180375058No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGTA277110.16016073986389892No Hit
TCTTTGGTTATCTAGCTGTATGATCTCGTAT275040.15896434589934236No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA271530.15693567787248555No Hit
AACATTCATTGCTGTCGGTGGATCTCGTATG270340.15624789583489024No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA263800.15246798446861007No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT263710.1524159673397163No Hit
TACCCTGTAGATCCGAATTTGTGTATCTCGT253450.14648601464582722No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT242830.14034799343636306No Hit
TGTCAGTTTGTCAAATACCCCAGATCTCGTA242690.14026707790252835No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTAT239350.1383366644524709No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT238960.13811125689393125No Hit
AACATTCATTGCTGTCGGTGGGATCTCGTAT237930.13751594975214707No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG237710.13738879677040675No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG232300.1342619893557927No Hit
TCCAGCATCAGTGATTTTGTTGATCTCGTAT231470.13378227583377245No Hit
AACATTCATTGCTGTCGGTGGGTATCTCGTA228250.13192121855557334No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT224770.12990988957168112No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT213640.12347710463181902No Hit
TATTGCACTTGTCCCGGCCTGTGAATCTCGT207760.12007865221075978No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT204930.11844300249110029No Hit
ACCACAGGGTAGAACCACGGACATCTCGTAT199070.11505610943201745No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGTA191570.11072134869087047No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT191480.1106693315619767No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA190760.1102531945308266Illumina Paired End PCR Primer 2 (95% over 24bp)
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA185640.10729399786487025No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG182440.10544449994864755No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT175450.10140450293789854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)