FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6430500
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA100085815.564232952336521Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT2564513.9880413653681677No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT1824272.836902262654537No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA1621502.5215768602752506No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT1360462.115636420185056No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1233291.9178757483865951No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1165081.8118031257289482No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1032051.6049296322214448No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT919741.4302775833916492No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT917061.4261099447943395No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT677941.0542570562164684No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT669861.0416919368633855No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA602230.9365212658424694No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT585160.9099758961200528No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT560300.8713163828629189No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG536840.8348339942461706No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGTA455250.7079542803825518No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC448280.697115309851489No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA406630.6323458518000156No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT401500.6243682450820309No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT392660.6106212580670244No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA389570.6058160329678874No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA388870.6047274706476946Illumina Single End Adapter 1 (95% over 23bp)
TGAGGTAGTAGATTGTATAGTTATCTCGTAT386220.6006064847212502No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG385130.5989114376798071No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT372130.5786952803047974No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC352880.5487598164994947No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT322990.5022782054272608No Hit
AACCCGTAGATCCGAACTTGTATCTCGTATG308640.4799626778633077No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC300910.46794183967032116No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG285240.4435735945882902No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC257760.4008397480755773No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT255060.3966410076976907No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT244630.38042142912681753No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG240430.37389005520566054No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT225220.3502371510768992No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT217790.3386828395925667No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA212400.33030090972708187No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT209760.3261954746909261No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA203090.3158230308685172No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT194650.3026980794650494No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT189280.2943472513801415No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT187370.29137703133504395No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT181250.2818598864785009No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT174080.2707098981416686No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG167270.2601197418552212No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA157520.2449576238239639No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG155930.24248503226809737No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG152810.23763315449809502No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC141360.21982738511779798No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT134740.2095326957468315No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG133990.20836637897519633No Hit
GGATATCATCATATACTGTAAGATCTCGTAT133120.2070134515200995No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA130630.20314127983827074No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG130110.20233263354327036No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG126940.19740300132182567No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG124150.1930643029313428No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG121710.18926988570095638No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT119380.1856465282637431No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT118740.18465127128528108No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA118370.18407588834460772No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG114290.17773112510691239No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG111860.17395225876681442No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA110360.17161962522354407No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT110060.17115309851488997No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG107660.1674208848456574No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA102920.16004976284892308Illumina Paired End PCR Primer 2 (95% over 24bp)
CACTAGATTGTGAGCTCCTGGAATCTCGTAT101350.15760827307363345No Hit
TGTCAGTTTGTCAAATACCCCAGATCTCGTA100870.15686183033978696No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT96790.15051706710209162No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT96760.1504704144312262No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT96400.14991058238084132No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT93230.14498095015939663No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT91130.14171526319881814No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA89880.13977140191275952Illumina Single End Adapter 1 (95% over 24bp)
AATGACACGATCACTCCCGTTGAATCTCGTA86780.1349506259233341No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT84830.13191820231708265No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA84790.13185599875592877No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA83550.1299276883601586No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT81790.12719073166938807No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGTA81320.12645983982583003No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA80450.1251069123707332No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT80120.12459373299121375No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT79750.1240183500505404No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG78860.12263432081486665No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA78600.12222999766736645No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG78410.12193453075188554No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT76520.1189954124873649No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA74150.11530985148899774No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAT74020.11510768991524764No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA72610.11291501438457352No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA72130.11216857165072701No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA71290.11086229686649561No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA70300.10932275872793719No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT67820.10546613793639685No Hit
GTAAACATCCTTGACTGGAAGCTATCTCGTA67400.10481300054428115No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT64840.10083197263043309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)