FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10655946
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA135959012.758979822157507Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT4065653.815381571941149No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT3392733.183884377792455No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA3290743.088172556429997No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA2989192.8051850112603796No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT2963432.781010714581324No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA2622832.461376962683557No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT2541232.384799998048038No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA2480042.3273766590033396No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA2185502.0509676006241024No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1401751.3154627472774354No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC1400211.314017544758579No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT1376311.2915887524204797No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1225321.1498932145489476No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT1205541.1313308081703868No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT1149331.0785809162321205No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT932690.8752765826703701No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT871730.818069085560306No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT848000.7957998285651974No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG776470.72867298689389No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG687280.644973238415435No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG640600.6011667101165865No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT625160.5866771472002579No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT619520.5813843275857442No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT606730.5693816391336818No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT529500.49690567125621693No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT472930.44381793976808814No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA457620.4294503744669877No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA419300.3934892312705038Illumina Single End Adapter 1 (95% over 23bp)
GTTTGTGATGACTTACATGGAATCTCGTATG413160.3877271900589586No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT376190.3530329451744594No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT366120.34358282221024766No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC343020.32190478442739856No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT339300.3184137757454852No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG326030.3059606345602727No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA313670.29436147668165735No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG308620.28962233855164055No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA299730.2812795785564229No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT299100.28068835934416336No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT295660.27746011475658755No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA294760.27661551588193106No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG293360.27530169541024324No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA292740.2747198606299244No Hit
AACATTCATTGCTGTCGGTGGGAATCTCGTA292010.27403479709825856No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT273960.25709589744542627No Hit
AACATTCAACGCTGTCGGTGAATCTCGTATG265410.24907220813618988No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG260600.24455829637274815No Hit
ACCATCGACCGTTGATTGTACATCTCGTATG257910.2420338841807194No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT251810.23630938069693674No Hit
AACATTCATTGCTGTCGGTGGATCTCGTATG249190.23385065952849235No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT242010.22711263739512194No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT240300.22550789953327466No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT239900.22513252225564956No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA235550.22105029436147666No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG231100.21687422214789753No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG223530.20977020716884265No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT217560.20416770130028813No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT213920.20075176807389977No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA202220.18977198270336582No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT194140.18218936169533892No Hit
GGATATCATCATATACTGTAAGATCTCGTAT187740.17618332525333744No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA185420.17400613704311188No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA180630.1695109941435514No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT179030.16800948503305102No Hit
AACATTCATTGCTGTCGGTGGGATCTCGTAT178210.16723996161391957No Hit
ACCACTGACCGTTGACTGTACCATCTCGTAT176090.1652504620425066No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC172210.1616093024495432No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA172150.16155299585789942No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG166870.1565980157932482No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT165260.1550871222508072No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG156760.14711035510127396No Hit
TAGCACCATTTGAAATCGGTTATCTCGTATG154960.14542115735196104No Hit
AACATTCATTGCTGTCGGTGGGTATCTCGTA154150.14466101836477024No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGTA153590.1441354901760951No Hit
TGTAAACATCCCCGACTGGAAGAATCTCGTA148930.13976234489176279No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT145280.1363370272334338No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA144450.13555811938236173No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA139310.13073452136487929No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT132560.12440002980495585No Hit
CAACGGAATCCCAAAAGCAGCTATCTCGTAT123670.11605726980973814No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC123670.11605726980973814No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA122490.11494990684074412Illumina Paired End PCR Primer 2 (95% over 24bp)
TTCACAGTGGCTAAGTTCCGCATCTCGTATG122140.11462145172282216No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCATC121730.11423669001325645No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT121170.11371116182458131No Hit
CTTATCAGATTGTATTGTAATTATCTCGTAT116530.10935678540413024No Hit
AACATTCATTGCTGTCGGTGGAATCTCGTAT116010.10886879494321762No Hit
TGTCAGTTTGTCAAATACCCCAGATCTCGTA115340.10824003800319558No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT115090.1080054272046799No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA115020.10793973618109551Illumina Single End Adapter 1 (95% over 24bp)
TGTAAACATCCTACACTCAGCTATCTCGTAT112290.10537778626130426No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG107680.10105156313667506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)