FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9009419
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA113744712.625087144909122Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT3283243.6442305547116853No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT2847623.160714359050234No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA2629602.9187231718271733No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA2345722.603630711369956No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT2149542.385880820949719No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT1404091.5584689756353878No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1362191.51196209211715No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT1230771.3660925304950298No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1104901.2263831885274734No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT1082211.201198434660437No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA1006221.1168533731198427No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT915671.0163474470440323No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC909061.0090106809329213No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA866660.961948822671029No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT865440.9605946842965124No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT828090.9191380709455294No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT687560.7631568694940262No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT617110.6849609281131225No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG614070.6815866816717038No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA543830.603623829683135No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT508780.5647201001529621No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG501110.5562067875853038No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG493410.5476601765330261No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT489750.543597761409476No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT484740.538036914478059No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT452250.5019746556353967No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG443170.4918963142906329No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT420610.46685585385694683No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG418040.4640032836745632No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA363580.403555434595727Illumina Single End Adapter 1 (95% over 23bp)
TAGCACCATTTGAAATCGGTTATCTCGTATG356750.39597447959740806No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG332550.36911370200453547No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT290590.32254022151705897No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT285250.3166130912548301No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT276870.3073117145511825No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA270160.29986395349134054No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC268710.2982545267347428No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT268120.29759965653723064No Hit
AATGACACGATCACTCCCGTTGAATCTCGTA260320.28894205053622213No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA258800.28725492731551283No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT241270.2677975128029898No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG239470.265799603725834No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT239060.26534452443603745No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT229470.25470010885274624No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT226240.25111497200873883No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG222980.24749653668011223No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG217650.2415805059127564No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA215110.23876123421499212No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA211040.23424373980164537No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT210420.23355557111951392No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG201020.22312204593881138No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT199470.22140162423348275No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT197420.21912622778449978No Hit
CAACGGAATCCCAAAAGCAGCTATCTCGTAT192140.21326569449150937No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT188770.2095251647192788No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG183100.203231751126238No Hit
CGTACCGTGAGTAATAATGCGATCTCGTATG179250.19895844560009918No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG177800.19734901884350145No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA176570.19598378097411165No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA174500.19368618553538247No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA171400.19024534212472524No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT168970.18754816487056492No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT168190.18668240427046406No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT162760.18065537855437738No Hit
TTCAAGTAATTCAGGATAGGTTATCTCGTAT159560.17710354019498928No Hit
CAACGGAATCCCAAAAGCAGCTGATCTCGTA159240.17674835635905045No Hit
TAGCACCATTTGAAATCGGTTAATCTCGTAT157280.17457285536392525No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT153530.17041054478651732No Hit
TTCACAGTGGCTAAGTTCCGCATCTCGTATG152790.16958918216590882No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA151490.1681462478324074No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC148530.16486079734997341No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTAT148060.16433912109093826No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG144910.1608427802059156No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTAT143640.15943314435703346No Hit
TGAGAACTGAATTCCATGGGTTATCTCGTAT141740.15732424033114675No Hit
AGTCTGTGATGAATTGCTTTGACTTCTGACA140920.15641408175155355No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT140830.15631418629769578No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTAT139340.1546603615616057No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA134240.1489996191763309No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGTA131310.14574746717851617No Hit
TTCAAGTAATCCAGGATAGGCTATCTCGTAT131290.14572526818877No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC130720.145092596981004No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG128290.14239541972684364No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC124740.13845509904689746No Hit
TGTCAGTTTGTCAAATACCCCAGATCTCGTA123450.13702326420826913No Hit
TAGCACCATTTGAAATCGGTATCTCGTATGC123260.13681237380568048No Hit
AACATTCAACGCTGTCGGTGAATCTCGTATG123000.1365237869389802No Hit
TGTAAACATCCTACACTCAGCTATCTCGTAT121510.1348699622028901No Hit
TGTAAACATCCCCGACTGGAAGAATCTCGTA120850.1341373955412663No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT120830.13411519655152013No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT119730.13289425211548048No Hit
ACCACTGACCGTTGACTGTACCATCTCGTAT114640.12724460922507877No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG114080.12662303751218587No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC112970.1253909935812731No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA105920.1175658496957462Illumina Paired End PCR Primer 2 (95% over 24bp)
AACCCGTAGATCCGAACTTGTGAATCTCGTA104520.1160119204135139No Hit
AACATTCATTGCTGTCGGTGGGAATCTCGTA104170.11562343809295582No Hit
CTCGTACGACTCTTAGCGGTGGATCATCTCG102510.11378092194402326No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA100070.11107264519498981Illumina Single End Adapter 1 (95% over 24bp)
TGAGAACTGAATTCCATAGGCTATCTCGTAT98940.1098184022743309No Hit
GGATATCATCATATACTGTAAGATCTCGTAT98200.1089970396537224No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT94810.10523431089174563No Hit
TGGCTCAGTTCAGCAGGAACAGAATCTCGTA93820.10413546089930995No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATGC93140.10338069524793995No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA91990.10210425333753487No Hit
TCGTATCCGGCTCGAAGGACCAATCTCGTAT90770.10075011496301815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)