FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8086492
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA114176614.119422859751793Illumina Single End Adapter 1 (95% over 24bp)
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT3404494.21009505728813No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA2847063.5207603000163727No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT2608773.226083696119405No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT2516613.1121158593862455No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT1879422.3241474795251142No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT1485931.8375458727962632No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT1460021.805504785016791No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1277961.580363895741194No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1100481.3608867726574143No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT894541.1062151548533037No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC803000.9930140288273333No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG720450.8909302080556067No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT714060.8830281412508664No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT700210.8659008133563973No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC643380.7956231206312947No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT610040.7543938706672807No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG548860.6787368366901247No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT528430.6534724822580669No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT494990.6121195692767643No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA489970.6059116858088773No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG487440.6027830114714762No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG468030.5787800198157619No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT421480.5212148852679258No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA409420.5063011253829225No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT402100.4972489925173982No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT361780.4473880639466409No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA354650.4385708908139648Illumina Single End Adapter 1 (95% over 23bp)
GGATATCATCATATACTGTAAGATCTCGTAT338310.41836435378901016No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT305070.37725876684228465No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG294960.36475643579440875No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG294700.36443491194945843No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT285830.353466002315961No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT270980.3351020442486062No Hit
TAGCACCATTTGAAATCGGTTATCTCGTATG270820.33490418342094447No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA270510.33452082806734984No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT259920.32142491453648875No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT246690.30506429734920903No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG243050.3005629635199045No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT237360.2935265378361841No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC234210.2896311527915937No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA221650.2740990778201475No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG214160.2648367178252325No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT211900.2620419336345105No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT210810.2606940067460649No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG201860.2496261666987366No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA199340.24650985866306427No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT199140.2462625326284871No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG196070.24246607799772757No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT195990.24236714758389669No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG179490.22196274973128025No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT177320.219279262256118No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA175170.2166205073844134No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG174650.21597745969451276No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT169550.20967064581279496No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT163290.20192934093052958No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT162730.2012368280337135No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC160720.19875120138621297No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA159320.19701991914417277No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA158990.19661183118712044No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTAT155260.19199920064225626No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC152470.18854900245990475No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTAT147040.18183410062113461No Hit
TCCTGTACTGAGCTGCCCCGAGTATCTCGTA146180.18077059867245276No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTAT144600.17881672299929313No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG143620.17760482542986503No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT141610.17511919878236448No Hit
TAGCACCATTTGAAATCGGTATCTCGTATGC138710.1715329712809955No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT135060.16701927114996218No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA131020.16202328525150336Illumina Paired End PCR Primer 2 (95% over 24bp)
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT129810.1605269627423115No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT128630.1590677391383062No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT128370.1587462152933559No Hit
TCCTGTACTGAGCTGCCCCGAATCTCGTATG128010.15830102843111699No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG124870.15441800968825542No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT124460.15391099131737224No Hit
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA123990.1533297751361159No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT115130.1423732318043473No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG114980.14218773727841444No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA113430.14027096051044136No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT112430.13903433033755552No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA111400.13776060125948308No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG110340.1364497732762241No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA110090.13614061573300265No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTAT109780.13575726037940802No Hit
TAGCACCATTTGAAATCGGTTAATCTCGTAT108380.13402597813736786No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT108230.13384048361143497No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT107150.13250492302471828No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT105270.13018005829969287No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA101380.12536956692716694No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA97860.12101662871860876Illumina Single End Adapter 1 (95% over 24bp)
TCAGTGCACTACAGAACTTTGTCATCTCGTA96210.11897618893334712No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA94940.1174056686137821No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA89590.11078969718884282No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT89310.1104434407404348No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA88410.10933047358483754No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGTA86420.10686957954079469No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC85520.10575661238519744No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA85110.10524959401431425No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC82650.10220748378901506No Hit
ACCACAGGGTAGAACCACGGACATCTCGTAT81940.10132947636626612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)