FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7680027
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA96583112.575880267087602Illumina Single End Adapter 1 (95% over 24bp)
CATTGCACTTGTCTCGGTCTGAATCTCGTAT3637664.736519806505888No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT3471904.52068723195895No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT2838813.6963541925047925No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA2508003.2656135193274713No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT1676972.1835470109675397No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT1588102.0678312719473513No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT1441851.8774022539243678No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1260611.6414135002389967No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA877821.1429907733397293No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG752100.9792934321715275No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT742930.967353369981642No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA621500.8092419466754479No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT619210.8062601863248657No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC590140.7684087568962974No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT571890.7446458196045405No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA556860.7250755759061784No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT549040.7148933200365051No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC526900.6860652963850258No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG504680.6571331064330893No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT476750.6207660467860334No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA476310.6201931321335199No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT425490.5540214897682001No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT361610.47084469885327224No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT313290.40792825337723426No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG313170.40777200392654867No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC305400.397654851994661No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT300400.3911444582160974No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG298830.38910019456962847No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA294080.3829153204799931No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA293030.38154813778649477Illumina Single End Adapter 1 (95% over 23bp)
TGTCAGTTTGTCAAATACCCCATATCTCGTA291950.380141892730325No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG282760.36817578896532527No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT267590.34842325424116344No Hit
TAGCACCATTTGAAATCGGTTATCTCGTATG252180.32835822061563064No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTAT243700.3173165927671869No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG240440.31307181602356343No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT233730.30433486757273115No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG233380.30387914000823174No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTAT233060.30346247480640365No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG231160.3009885251705495No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT222740.29002502204744857No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT216340.2816917180108872No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA202720.2639574053580801No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT202720.2639574053580801No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT202180.2632542828299953No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT195720.25484285406809115No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT185640.24171790021050707No Hit
GGATATCATCATATACTGTAAGATCTCGTAT183810.23933509608755282No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA182630.2377986431558118No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT180260.23471271650477268No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT179680.23395751082645935No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT179640.23390542767623082No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG177980.23174397694174773No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC170070.2214445339840602No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT167820.2185148567837066No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT163950.21347581199909843No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT160730.20928311840570352No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG158700.20663989853160672No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT155640.20265553753912582No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG152710.1988404467848876No Hit
TGTCAGTTTGTCAAATACCCCAATATCTCGT132410.17240824804391963No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT131060.17065044172370747No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGTA127590.1661322284413844No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTAT122560.15958277230014944No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG121590.15831975590710815No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA119840.1560411180846109Illumina Paired End PCR Primer 2 (95% over 24bp)
TACCCTGTAGATCCGAATTTGTATCTCGTAT119490.15558539052011144No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA117120.15249946386907234No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT115050.14980416084474701No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT111830.1456114672513521No Hit
TGTCAGTTTGTCAAATACCCCAGATCTCGTA111660.14539011386288095No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA111230.14483021999792448No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATGC111070.14462188739701046No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG110850.14433543007075367No Hit
TTCACAGTGGCTAAGTTCCGCATCTCGTATG109000.14192658437268516No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA107400.1398432583635448No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA107320.1397390920630878No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTATG104480.13604118839686372No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA104210.13568962713282126No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC104130.13558546083236425No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCAT99300.12929642044227188No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG97790.1273302815211457No Hit
TAGCACCATTTGAAATCGGTATCTCGTATGC96420.1255464336258193No Hit
ACCACAGGGTAGAACCACGGACATCTCGTAT94740.12335894131622192No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG94740.12335894131622192No Hit
TAGCACCATTTGAAATCGGTTAATCTCGTAT91780.11950478819931232No Hit
AATGACACGATCACTCCCGTTGAATCTCGTA91000.11848916676985642No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT89970.11714802565147232No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT88460.11518188673034613No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT88200.11484334625386082No Hit
CTTTCAGTCGGATGTTTACAATCTCGTATGC87980.11455688892760404No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC86820.1130464775709773No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT85980.11195273141617862No Hit
CATTGCACTTGTCTCGGTCTGATATCTCGTA85120.11083294368626569No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT84640.11020794588352358No Hit
TAGCACCATCTGAAATCGGTATCTCGTATGC84050.1094397194176531No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG82970.10803347436148336No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGTA82960.10802045357392624No Hit
ACCACAGGGTAGAACCACGGACAATCTCGTA80210.10443973699571629No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA80200.10442671620815916Illumina Single End Adapter 1 (95% over 24bp)
TTCAAGTAATCCAGGATAGGATCTCGTATGC78420.10210901602299054No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT78120.10171839239627672No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)