FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11017536
Sequences flagged as poor quality0
Sequence length31
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA122217611.093006639597093Illumina Single End Adapter 1 (95% over 24bp)
TACCCTGTAGATCCGAATTTGTATCTCGTAT10901169.894372026558388No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT6250035.672801976775933No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT5052574.585934640921527No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA2944452.6725122568240303No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT2235942.0294374350126922No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT2082321.8900051699399938No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA1943941.7644053988114947No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1814201.6466476714938802No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1665141.5113542628769263No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1624231.474222548489971No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1227381.114024043125432No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG1158001.0510517052088597No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA1048260.9514468570831082No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA895760.8130311532451538No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG831340.7545607293681637No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT739720.6714023897902399No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT685280.6219902526299891No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT613040.5564220529889805No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT607010.5509489599126338No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT559470.5077995660735758No Hit
TCTTTGGTTATCTAGCTGTATGATCTCGTAT555790.5044594363022731No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG506960.4601391817553399No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA474060.43027769548472544No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT457500.415247111513863No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG452820.41099933778296704No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA452600.4107996561118566Illumina Single End Adapter 1 (95% over 23bp)
CTTTCAGTCGGATGTTTACAGCATCTCGTAT450430.4088300687195395No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC419610.38085648188487875No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT411850.373813164758436No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT369880.3357193477743118No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGTA367070.333168868247855No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT360900.32756870501716534No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA358850.3257080348999994No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA358050.32498191973232493No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT352880.32028940046122834No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT350240.31789322040790247No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA333900.3030623181081505No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT308680.2801715374472114No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA300210.27248379310945753No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG297350.2698879313850211No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA291010.26413346868120063No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTAT285160.2588237515175807No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT270700.24569921986186383No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTAT270090.24514555704651203No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG268500.24370240315075894No Hit
TCGTATCCGGCTCGAAGGACCAATCTCGTAT261070.2369586085309819No Hit
TACCCTGTAGATCCGAATTTGTGATCTCGTA226130.20524552858279746No Hit
TACCCTGTAGATCCGAATTTGTGTATCTCGT225220.2044195725795677No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT217930.1978028481141337No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT211200.19169440426607184No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT205610.18662067453194617No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG203540.18474185153558836No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA200750.18220952488832348No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG200720.1821822955695357No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT199770.1813200338079222No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC187720.17038292409482483No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT187010.1697384968835137No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG182610.16574486346130388No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA180020.16339406560595762Illumina Paired End PCR Primer 2 (95% over 24bp)
TCGTATCCCACTTCTGACACCAATCTCGTAT178540.1620507525457598No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT175650.1594276615025356No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT175480.15927336202940476No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT164590.14938911930943544No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG161560.1466389581118682No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT157180.14266347756885023No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG152940.13881506718017533No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA148840.13509372694584343No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT144230.13090948829211904No Hit
TAGCTTATCAGACTGATGTTGACAATCTCGT143880.13059181290626143No Hit
TCGTATCCCACTCCTGACACCAATCTCGTAT141470.12840439096364198No Hit
ACTTAACTTGACCGCTCTGACCAATCTCGTA140120.12717907161819123No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC135090.1226136225014377No Hit
TTAATGCTAATCGTGATAGGGGTATCTCGTA129010.11709514722711141No Hit
TTATGGTTTGCCTGGGACTGAATCTCGTATG127640.11585167500246878No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG123100.11173097142591591No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA123090.11172189498631999No Hit
CTTATCAGATTGTATTGTAATTATCTCGTAT122180.11089593898309022No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA118770.10780087308087762No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG117350.10651201865825535No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT117210.10638494850391232No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA117140.10632141342674079Illumina Single End Adapter 1 (95% over 24bp)
AAGACTTTTTCTCTGACCAATCTCGTATGCC116500.10574052129260118Illumina PCR Primer Index 4 (95% over 22bp)
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA112480.10209179257503675No Hit
CAACGGAATCCCAAAAGCAGCTGATCTCGTA112320.10194656954150183No Hit
ATCACATTGCCAGGGATTTCCAATCTCGTAT111660.10134752452817036No Hit
TTCACAGTGGCTAAGTTCCGCATCTCGTATG111470.10117507217584766No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG111370.10108430777988835No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGTA111190.10092093186716158No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)