FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11405358
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTGTAGATCCGAATTTGTATCTCGTAT129359711.342011359923994No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA128384911.256542758236963Illumina Single End Adapter 1 (95% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTAT5637014.94242267537766No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT4247263.723916425946472No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT2603522.282716596883675No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT2065341.810850654578313No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1922161.685313165969889No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA1773701.5551462742335664No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA1697181.4880550001148583No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1589011.3932136106556234No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG1558861.3667786666582495No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1330861.1668726224990045No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1187961.0415806325413022No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA1092370.9577691467466432No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT1066650.9352183421160476No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT961780.8432703296117492No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT737820.6469064802700626No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG666220.5841289681568961No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC647580.5677857722659824No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG629810.5522053757540973No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT575270.5043857457170569No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT534640.4687621379355212No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT516790.4531115989520013No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA485790.4259313911935075Illumina Single End Adapter 1 (95% over 23bp)
CTTTCAGTCGGATGTTTACAGTATCTCGTAT482990.4234764046862887No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT473030.4147436669677532No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT456780.4004959774169299No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG441700.3872741215137657No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGTA425400.37298259291817054No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT410090.35955907740905635No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG377530.33101109145368346No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC350930.3076887196351048No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG348880.3058913187994625No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT348330.3054090893069731No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT338320.2966325125436659No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA333720.2925993204246636No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT315870.2769487814411437No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA304090.2666203024929161No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT303950.26649755316755513No Hit
TACCCTGTAGATCCGAATTTGTGTATCTCGT295350.25895723746681165No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT287700.25224986361673174No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA285870.2506453545780852No Hit
TCGTATCCCACTTCTGACACCAATCTCGTAT279880.24539343701442778No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG273920.24016782287763347No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA266420.23359196616186884No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT265160.23248722223362037No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTAT258610.22674430736851925No Hit
TACCCTGTAGATCCGAATTTGTGATCTCGTA256260.22468387226424633No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA253390.22216751109434707No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT251070.2201333794169372No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG235190.20621009879742488No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA228600.20043211269650635No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTAT216230.18958633301997185No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTAT212720.18650883207699398No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG209100.18333488523551825No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA207700.18210739198190887No Hit
TCGTATCCGGCTCGAAGGACCAATCTCGTAT206930.18143227069242368No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA206900.1814059672655606No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA204680.17945951367769428No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC201720.17686424222720584No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT191810.16817534355344216No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT184380.161660861500358No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG180500.15825895162606907No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA174910.15335774642058583No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG173170.15183214766252845No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT171020.14994706873734256No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC162280.14228400371123817No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT160510.1407321015263177No Hit
TCGTATCCCACTCCTGACACCAATCTCGTAT159900.1401972651801022No Hit
AATGACACGATCACTCCCGTTGAATCTCGTA158960.13937309113839302No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA158300.13879441574740572Illumina Paired End PCR Primer 2 (95% over 24bp)
TAGCTTATCAGACTGATGTTGACAATCTCGT156690.1373827985057549No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT151430.13277093099576534No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG145090.12721214011870563No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT144720.12688773118739455No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT143070.12544104270992634No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT142990.1253709002382915No Hit
TTAATGCTAATCGTGATAGGGGTATCTCGTA142900.12529198995770235No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT135890.11914575588070099No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG135420.1187336688598464No Hit
CAACGGAATCCCAAAAGCAGCTGATCTCGTA133960.1174535687525109No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT132190.11590166656759043No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT127340.1116492792247293No Hit
TCGTTTCCCGGCCAACGCACCAATCTCGTAT122890.10774760424004227No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA120500.10565209789995193No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTAT119310.10460872863438395No Hit
TCGGATCCCACTTCTGACACCAATCTCGTAT119040.10437199779261641No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG117070.1026447394286089No Hit
TCGTTTCCCGGGCGGCGCACCAATCTCGTAT114700.10056676870642726No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA114630.10050539404374681Illumina Single End Adapter 1 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)