FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10875620
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTGTAGATCCGAATTTGTATCTCGTAT133144712.242492841787412No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA114492210.527418206961995Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT5418164.981932064562756No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT3021572.7782967775630265No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1990721.8304427701593105No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG1779091.6358515652440964No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1643321.511012705482538No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA1542541.4183467241407848No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1346801.2383661805028126No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT1263321.1616073382483023No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1089261.001561290298852No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1000710.9201406448551899No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA954650.8777890363951664No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA798370.7340914816810443No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG722380.6642196031122823No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT691840.6361384454403519No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG669890.6159556880435322No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT596840.548787103631793No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT587090.5398220974988093No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT571190.525202241343482No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG495900.45597400424067774No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT489700.45027317982790865No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC479670.4410507171085419No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT472540.43449476903385736No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT464530.4271296716876831No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG455400.4187347479959763No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA417590.38396891395616983Illumina Single End Adapter 1 (95% over 23bp)
TATTGCACTTGTCCCGGCCTGTAAATCTCGT413700.38039210638106147No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG397970.3659285631531812No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT386410.3552992840867923No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT375410.34518491819316965No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA375150.3449458513629568No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT372110.3421506084250829No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT369790.34001739670933706No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT363360.33410509010061035No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGTA359810.33084090838039576No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA358650.3297743025225229No Hit
TAGCACCATTTGAAATCGGTTATCTCGTATG340720.313287886115918No Hit
TACCCTGTAGATCCGAATTTGTGATCTCGTA336020.3089662934159156No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT333860.3069801997495315No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG333550.30669515852889306No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTAT325360.2991645533771868No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA323070.29705892629569625No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC322350.2963968950735682No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTAT318590.2929396209135663No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT306490.2818138184305814No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA288190.2649871915348274No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT281940.25924039273163274No Hit
AGTCTGTGATGAATTGCTTTGACTTCTGACA277020.25471651271375795No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT265300.2439401155980073No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT264550.24325049974162394No Hit
TACCCTGTAGATCCGAATTTGTGTATCTCGT262510.2413747446122612No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA251190.230966142619915No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA234180.2153256549971404No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT234090.21524290109437438No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC231220.21260397108394738No Hit
TTAATGCTAATCGTGATAGGGGTATCTCGTA222630.20470557080883664No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG221820.20396078568394263No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT218470.20088050152543027No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG217860.2003196139622385No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA202280.1859939939056348No Hit
TAGCACCATTTGAAATCGGTTAATCTCGTAT200760.18459637243669785No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT193040.1774979265549918No Hit
TTCACAGTGGCTAAGTTCCGCATCTCGTATG189950.17465670922669235No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT184500.16964550067030662No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT180840.16628017529115582No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT176730.16250108039817499No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT167120.15366480255838288No Hit
TCGTATCCCACTTCTGACACCAATCTCGTAT162700.1496006664447636No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG160650.14771571643731576No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTAT160010.14712724423986862No Hit
TTAATGCTAATCGTGATAGGGGATCTCGTAT155010.1425298051973129No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG153620.1412517171434824No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG146980.13514631809496838No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTAT144140.13253497271879672No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG143250.13171662856922178No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT140360.1290593088026246No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA136810.12579512708241No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG135810.12487563927389886No Hit
CAACGGAATCCCAAAAGCAGCTGATCTCGTA134850.12399293097772816No Hit
AATGACACGATCACTCCCGTTGAATCTCGTA126670.11647152070410698No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA123590.11363949825389265No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA121010.11126721970793388No Hit
TGTAGTGTTTCCTACTTTATGGAATCTCGTA120520.11081667068176342No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCG114870.10562156456367544No Hit
CAACGGAATCCCAAAAGCAGCTATCTCGTAT113240.10412279943580229No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTAT112770.10369064016580204No Hit
ATACCCTGTAGATCCGAATTTGTATCTCGTA112040.1030194140655889No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)