FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20000000
Sequences flagged as poor quality0
Sequence length31
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTGTAGATCCGAATTTGTATCTCGTAT329266516.463325No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA19569879.784935Illumina Single End Adapter 1 (95% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTAT8772774.386385000000001No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT8635164.31758No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA5786392.893195No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG5342042.67102No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT4289252.144625No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA3209281.6046399999999998No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT3154791.5773949999999999No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA2516931.258465No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT2424751.212375No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA2402281.20114No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA2093201.0466No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA1769800.8848999999999999No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT1754360.87718No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC1456470.728235No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG1423620.7118099999999999No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1405280.70264No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA1308510.654255No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGTA1306340.6531699999999999No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT1257390.628695No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC1119750.559875No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT1111060.55553No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT1091530.545765No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT1077820.53891No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT1067700.53385No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG1020010.510005No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG956490.478245No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT898010.449005No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG894740.44737No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT847270.423635No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT824350.41217500000000007No Hit
TACCCTGTAGATCCGAATTTGTGTATCTCGT782730.391365No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT775130.387565No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTAT741480.37074No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG712940.35647No Hit
TACCCTGTAGATCCGAATTTGTGATCTCGTA684070.342035No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA677280.33864No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA672080.33604Illumina Single End Adapter 1 (95% over 23bp)
TCAGTGCACTACAGAACTTTGTATCTCGTAT652320.32616No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT643510.321755No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG628560.31428No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT614940.30747No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT614440.30722No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC565930.282965No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA548490.274245No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT529760.26488No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG517750.25887499999999997No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA516890.258445No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA501600.25079999999999997No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA486470.24323499999999998No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT476730.23836500000000002No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT470720.23535999999999999No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC445780.22288999999999998No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA414240.20712No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA405620.20281000000000002No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA395550.19777499999999998No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT356790.178395No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT353340.17667No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT332350.16617500000000002No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT304520.15226No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTAT288400.1442No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG287530.143765No Hit
TCTTTGGTTATCTAGCTGTATGATCTCGTAT278790.139395No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT278570.139285No Hit
TATTGCACTTGTCCCGGCCTGTGAATCTCGT262740.13137No Hit
AACATTCATTGCTGTCGGTGGGAATCTCGTA261540.13077No Hit
ATACCCTGTAGATCCGAATTTGTATCTCGTA246600.12329999999999999No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT245610.12280500000000001No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG225520.11276000000000001No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG219410.109705No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG218210.10910500000000001No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG210780.10539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)