FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19344262
Sequences flagged as poor quality0
Sequence length31
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTGTAGATCCGAATTTGTATCTCGTAT261355913.510771307791428No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA202508410.468654735962529Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT10118675.230837961148375No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT6226953.2190165745273718No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA5499132.842770636584637No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT3749871.938492148214287No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG3718931.9224977411906436No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA2911771.5052370568595483No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT2683411.3871865465842015No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT2566771.3268895965118752No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA2238251.157061458328056No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1877960.9708098453174383No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1748150.9037046748022746No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT1613620.8341595042498907No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT1574170.8137658598710046No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC1459080.7542701809973417No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT1432750.7406589096032714No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT1355220.7005798411952857No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA1348850.6972868750433592No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG1289720.6667196711872493No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA1235620.6387527216080924No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT1171160.605430178726901No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT1107490.5725160256824479No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA1069380.5528150931785353No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG1060370.5481573812430787No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT1051250.543442804899975No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT1023880.5292939063790596No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT1010260.5222530588140297No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA990270.5119192450970732No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG938930.4853790752006978No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT890820.46050865109250483No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT831880.4300396675768763No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT813540.4205588199746261No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGTA813260.4204140742097062No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG802260.4147276334449978No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC775570.4009302603531734No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC744880.385065090619637No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT690110.3567517851029933No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG682940.35304525962272426No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA667680.3451566154345925No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA647880.33492102205811725Illumina Single End Adapter 1 (95% over 23bp)
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA605880.3132091573201397No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT579630.29963924185890367No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG572000.29569491976483775No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC526420.2721323770325278No Hit
TACCCTGTAGATCCGAATTTGTGTATCTCGT516350.26692669898701743No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT487850.25219364791481835No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTAT473400.24472373254663318No Hit
TACCCTGTAGATCCGAATTTGTGATCTCGTA472450.24423263084422656No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA470700.2433279698134775No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT411120.21252813883517502No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG409520.21170102017849013No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG397560.20551830821977082No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG385210.1991339860884845No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT380520.19670949452607703No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC368020.19024763002072656No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA366170.1892912740739347No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT363120.18771457913462916No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG361560.18690813844436144No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA355680.1838684773810446No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTAT347080.17942271460136344No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA344080.17787186712007935No Hit
TAGCACCATTTGAAATCGGTTATCTCGTATG324110.1675483923863314No Hit
TGTCAGTTTGTCAAATACCCCAGATCTCGTA318860.16483440929408422No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG303670.1569819515471823No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT303380.15683203629065817No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG293830.1518951718085704No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT293710.15183313790931907No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT281620.14558322255974407No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT271240.14021729027450103No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT263800.13637118852091643No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT239930.12403161206149917No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA238860.12347847645984118No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT225060.11634457804593425No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT223070.11531584921668245No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA218950.11318601867571892No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA216130.11172822204331187No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT214500.11088559491181417No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT209860.10848695080742805No Hit
TACCCTGTAGATCCGAATTTGTAATCTCGTA209690.1083990694501553No Hit
TATTGCACTTGTCCCGGCCTGTGAATCTCGT206390.10669313722074278No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA205290.10612449314427191No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA203750.10532839143721275No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC202910.1048941541424532No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG201930.10438754396523373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)