FastQCFastQC Report
Wed 8 May 2024
EGAF00007772546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935400
Total Bases28.9 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTGTAGATCCGAATTTGTATCTCGTAT14315615.304254864229208No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA10250810.958734231344879Illumina Paired End PCR Primer 2 (100% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT379964.062005559119093No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA254772.7236476373743854No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG187902.0087663031858027No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA182661.9527474877057944No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT168251.798695745135771No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA144101.5405174257002352No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT143911.5384862091084028No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT143221.531109685695959No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA131701.40795381654907No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT109521.1708360059867438No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA83220.8896728672225785No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG77170.8249946546931795No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT70580.7545435107975198No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT62680.670087663031858No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT59540.6365191361984178No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT59390.6349155441522344No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT57980.6198417789181099No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC57650.6163138764165064No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA56490.6039127645926876No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA56030.598995082317725Illumina Paired End PCR Primer 2 (100% over 23bp)
ACCCTGTAGATCCGAATTTGTGTATCTCGTA55540.5937566816335257No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT55490.5932221509514646No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT54620.5839213170836006No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT52560.5618986529826812No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG50450.5393414581997007No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT49030.524160786829164No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT47970.5128287363694677No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG47920.5122942056874065No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT43740.4676074406670943No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT41930.44825742997648066No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA35310.37748556767158437No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT35050.3747060081248664No Hit
TACCCTGTAGATCCGAATTTGTGTATCTCGT33900.3624118024374599No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTAT33260.3555698097070772No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA31640.3382510156082959No Hit
TACCCTGTAGATCCGAATTTGTGATCTCGTA31520.33696814197134917No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT30610.3272396835578362No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG30220.32307034423775927No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT29250.31270044900577293No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA29080.310883044686765No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT29070.3107761385503528No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT28070.30008552490912976No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG26750.28597391490271545No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA26460.28287363694676076No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT26440.2826598246739363No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT24150.2581783194355356No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC23860.2550780414795809No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA23680.2531537310241608No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA22290.2382937780628608No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG21790.23294847124224932No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT20270.21669873850759033No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT19940.21317083600598674No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG19720.21081890100491768No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC18330.19595894804361771No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT17390.18590977122086808No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA15960.17062219371391918TruSeq Adapter, Index 20 (100% over 24bp)
CTTTCAGTCGGATGTTTACAGATCTCGTATG14760.15779345734445158No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA14350.15341030575155015No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT13880.14838571734017533No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG13740.1468890314304041No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT13670.14614068847551848No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG13560.14496472097498395No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG13040.13940560188154802No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT12990.13887107119948686No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT12820.13705366688047893No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC12530.13395338892452427No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG12500.1336326705152876No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA12470.13331195210605087No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT11890.12711139619414152No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT11810.12625614710284372No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA11770.1258285225571948No Hit
CAACGGAATCCCAAAAGCAGCTATCTCGTAT11750.12561471028437032No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG11520.12315586914688904No Hit
TTCACAGTGGCTAAGTTCCGCATCTCGTATG11420.12208680778276672No Hit
TGTCAGTTTGTCAAATACCCCAGATCTCGTA11090.11855890528116313No Hit
CAACGGAATCCCAAAAGCAGCTGATCTCGTA11060.11823818687192644No Hit
TACCCTGTTGATCCGAATTTGTATCTCGTAT10890.11642078255291854No Hit
TATTGCACTTGTCCCGGCCTGTGAATCTCGT10770.11513790891597177No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG10640.11374812914261279No Hit
ATACCCTGTAGATCCGAATTTGTATCTCGTA10480.11203763096001709No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT10210.1091511652768869No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCATC10200.10904425914047466No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA10100.10797519777635237No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA10090.10786829163994013No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA9840.10519563822963439No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTAT9640.10305751550138977No Hit
TCGTATCCGGCTCGAAGGACCAATCTCGTAT9470.10124011118238187No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)