FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793406
Sequences flagged as poor quality0
Sequence length31
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTGTAGATCCGAATTTGTATCTCGTAT10299212.980995858362554No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA8817511.113477841105311Illumina Single End Adapter 1 (95% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTAT367324.629659972321863No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT316313.9867356687496693No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA171662.163583335644046No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT152081.9167992175506614No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA135011.7016508571903919No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT122941.5495219345454911No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG121741.5343972694938026No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT119081.5008709286292266No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA118141.489023274338737No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT115691.4581437498582062No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA107751.3580688827662004No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA104941.322651958770163No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT79020.9959591936536906No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT62020.7816931054214361No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA54630.6885503764781209Illumina Single End Adapter 1 (95% over 23bp)
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT53220.6707788950423869No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT53170.6701487006652332No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG42990.541841125476742No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT39410.4967192080725379No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG37200.4688646166023448No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGTA36230.45663884568556323No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT31850.4014338182469001No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT31530.3974005742331165No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC30950.39009031945813366No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT30910.3895861639564107No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT30070.3789988984202287No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT29930.37723435416419837No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT29550.3724448768978304No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC28540.35971495047932583No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT28110.3542952788358041No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA27050.3409351580401459No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC25920.3266927651164725No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT25500.3213991323483816No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG24750.3119462166910762No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTAT24250.30564427291953933No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA22910.2887550636118204No Hit
TACCCTGTAGATCCGAATTTGTGTATCTCGT22120.27879799245279213No Hit
TACCCTGTAGATCCGAATTTGTGATCTCGTA21510.27110962105151715No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA21390.26959715454634825No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT19840.25006112885458387No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA19430.24489353496192365No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTAT18930.23859159119038678No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG18820.23720516356064864No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA17610.22195445963352936No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG17570.2214503041318064No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG16970.21388797160596215No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT16380.20645167795554858No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA15500.1953602569176437No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG15420.19435194591419777No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA15270.1924613627827367No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG15040.18956246864782975No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT14920.18805000214266088No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT14470.18237825274827768No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG14240.17947935861337072No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT14210.1791012419870785No Hit
AATGACACGATCACTCCCGTTGAATCTCGTA14180.17872312536078627No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT14150.17834500873449408No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT14040.17695858110475596No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC14040.17695858110475596No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA13980.17620234785217154No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT13290.1675056654474506No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA12970.163472421433667Illumina Paired End PCR Primer 2 (95% over 24bp)
TTTGGCAATGGTAGAACTCACAATCTCGTAT12530.15792671091471455No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA12260.15452366127808462No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA12130.15288515589748503No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT12030.15162476714317763No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG11790.14859983413283992No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG11120.1401552294789805No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG10870.13700425759321205No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT10630.13397932458287434No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG10560.13309705245485917No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT9970.12566075880444566No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA9780.12326602017126162No Hit
ATACCCTGTAGATCCGAATTTGTATCTCGTA9720.12250978691867719No Hit
TCGTATCCCACTTCTGACACCAATCTCGTAT9540.12024108716092391No Hit
TCGTATCCGGCTCGAAGGACCAATCTCGTAT9420.11872862065575507No Hit
TAGCTTATCAGACTGATGTTGACAATCTCGT9280.11696407639972474No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA9020.11368706563852556No Hit
TTCACAGTGGCTAAGTTCCGCATCTCGTATG8780.11066213262818783No Hit
TACCCTGTTGATCCGAATTTGTATCTCGTAT8750.11028401600189564No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG8730.11003193825103415No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT8670.10927570499844971No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTAT8510.10725908299155792No Hit
TTCACCACCTTCTCCACCCAGCATCTCGTAT8390.10574661648638906No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT8100.10209148909889765No Hit
TGTCAGTTTGTCAAATACCCCAGATCTCGTA7970.10045298371829808No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA7940.10007486709200586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)