FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000546
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA200074612.504235792953565Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT10115766.322134257168474No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA6355003.9717394643907777No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT5717753.5734718052746453No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT5533113.458075743165265No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT3321752.076022905718342No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA2818101.7612523972619434No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT2547891.5923769101379417No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA2450431.531466488706073No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT2252641.4078519570519654No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA2043451.2771126685301866No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA1788671.1178806023244456No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC1633141.0206776693745325No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG1487240.9294932810417844No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT1403540.8771825661449304No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT1379500.8621580788555591No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG1364070.852514657937298No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGTA1333660.8335090565034469No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT1326110.8287904675252956No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT1250650.7816295768906886No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG1156060.7225128442491899No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA1126050.7037572342843801No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT1097440.6858765944612141No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT1095120.6844266439407755No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC1042670.6516465125627589No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT926410.5789864920859575No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT912790.5704742825651075No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT887840.5548810646836677No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT886040.5537561030729826No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA851880.5324068316168711No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT815510.5096763572943073No Hit
AACCCGTAGATCCGAACTTGTATCTCGTATG813830.5086263931243346No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA813220.5082451561340469Illumina Single End Adapter 1 (95% over 23bp)
ACCATCGACCGTTGATTGTACCATCTCGTAT787810.4923644480632099No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT723480.45215957005467183No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT711060.44439733494094513No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG675020.42187310358034036No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT592670.37040610989149997No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA546160.34133835182874384No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT522770.3267201006765644No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT515370.3220952584993037No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA508500.3178016550185225No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG503950.31495800205817975No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT493470.3084082255693025No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG488120.30506458967087746No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG465240.29076507764172543No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT458490.2865464716016566No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC457400.28586524484851955No Hit
TTAATGCTAATCGTGATAGGGGATCTCGTAT407140.2544538167635029No Hit
TTAATGCTAATCGTGATAGGGGTATCTCGTA396140.24757905136487218No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA396010.2474978041374338No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT370540.23157959734624056No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG370070.23128585737011725No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG367110.22943592049921296No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA364030.22751098618759633No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA362290.22642352329726748No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA352580.2203549803862943No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT352190.22011123870397925No Hit
TATTGCACTTGTCCCGGCCTGTGAATCTCGT324760.2029680737144845No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT308760.19296841495283973No Hit
AACATTCATTGCTGTCGGTGGGAATCTCGTA300320.18769359495607213No Hit
ACCACTGACCGTTGACTGTACCATCTCGTAT288600.18036884491316735No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA284150.1775876898200849No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT275170.17197538134011175No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA274480.17154414605601584No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT271840.16989420236034444No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG260980.16310693397587808No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT254090.1588008309216448No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG224420.14025771370551982No Hit
TTAATGCTAATCGTGATAGGGATCTCGTATG221430.13838902747443743No Hit
TCGTATCCCACTTCTGACACCAATCTCGTAT212740.13295796280951913No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT208240.13014555878280654No Hit
TAGGTAGTTTCCTGTTGTTGGGATCTCGTAT201760.12609569698434042No Hit
TGAGAACTGAATTCCATGGGTTATCTCGTAT200420.12525822556305266No Hit
ACCATCGACCGTTGATTGTACATCTCGTATG196660.12290830575406614No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG195370.12210208326640855No Hit
AACATTCATTGCTGTCGGTGGGTATCTCGTA191200.11949592220165486No Hit
TATTGCACTTGTCCCGGCCTGTAATATCTCG190070.1187896963016137No Hit
CATTGCACTTGTCTCGGTCTGATATCTCGTA189650.11852720525912053No Hit
AACATTCATTGCTGTCGGTGGGATCTCGTAT185670.11603979014216141No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA184580.11535856338902434Illumina Paired End PCR Primer 2 (95% over 24bp)
CGCGACCTCAGATCAGACGTGGATCTCGTAT184420.11525856680140789No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC179360.11209617471803775No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGTA177910.1111899556427637No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT176270.11016499061969509No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA175180.10948376386655805Illumina Single End Adapter 1 (95% over 24bp)
CTTATCAGATTGTATTGTAATTATCTCGTAT173640.10852129671074975No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT170780.10673385770710575No Hit
TAACTGGTTGAACAACTGAACCATCTCGTAT169830.10614012796813309No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTAT169260.1057838901247495No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG169020.10563389524332481No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG165060.10315897969981774No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA163150.10196527043514642No Hit
TCGTATCCGGCTCGAAGGACCAATCTCGTAT161490.10092780583862576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)