FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5762621
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA109373218.979766325080202Illumina Single End Adapter 1 (95% over 24bp)
TACCCTGTAGATCCGAATTTGTATCTCGTAT2947925.115588896094329No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA2747354.76753546693423No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT2633734.570368240423932No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT2080003.6094686775340596No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA1497782.5991298056908483No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1427812.4777093617643775No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA926971.6085909519296862No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT762851.3237899907004123No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT756101.3120765707132225No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT550180.9547391716373504No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT526750.9140805893707048No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA483080.8382991003572853Illumina Single End Adapter 1 (95% over 23bp)
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC481760.8360084760042349No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG439340.7623961388402951No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT437950.7599840419836738No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT401870.6973736430002945No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG386600.6708752840070517No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT356850.6192494699894371No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT310420.5386784936923668No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA287920.49963376040173385No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG283590.4921198183951365No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA270200.468883863783511No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG268110.46525704189118117No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG262600.455695420538675No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA258370.448355010680036No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT256700.44545702380913127No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT252540.43823808645406315No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA223850.3884517132048073No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT209850.36415721249063576No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG190650.33083904008262904No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT190080.3298499068392664No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT185230.3214335976632855No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA179020.3106572512750708No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA177770.30848809942559124No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT166970.2897466274460875No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG156930.2723239997910673No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT149530.259482620842148No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA147280.2555781475130847No Hit
TAGGTAGTTTCCTGTTGTTGGGATCTCGTAT146070.2534784085227885No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGTA127410.221097309713757No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA126970.22033376826274018No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTAT119920.20809975183167523No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT119060.20660737535923324No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA118020.2048026410204662Illumina Paired End PCR Primer 2 (95% over 24bp)
CTCGGCGTGGCGTCGGTCGTGGATCTCGTAT115170.19985697480365272No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT114870.19933637835977763No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT114350.19843401119039408No Hit
CTAAGCCAGGGATTGTGGGTATCTCGTATGC112530.19527572609755178No Hit
CAACGGAATCCCAAAAGCAGCTGATCTCGTA106110.18413496219862455No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTAT106080.18408290255423704No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG102200.17734985521345234No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA90560.15715071319109827Illumina Single End Adapter 1 (95% over 24bp)
TACCCTGTAGATCCGAATTTGTGAATCTCGT87360.15159768445643051No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT85260.14795350934930476No Hit
TGTTTGTGATGACTTACATGGAATCTCGTAT84040.14583641714421267No Hit
TACCCTGTAGATCCGAATTTGTGATCTCGTA82130.14252195311820784No Hit
CTTATCAGATTGTATTGTAATTATCTCGTAT81240.14097751700137837No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT80430.13957190660291557No Hit
TAACTGGTTGAACAACTGAACCCATCTCGTA79210.13745481439782348No Hit
TATTGCACTTGTCCCGGCCTGTAATATCTCG77600.13466094681569377No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG76380.13254385461060167No Hit
CTTCGTGATCGATGTGGTGACGTATCTCGTA75950.1317976663743807No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA75560.13112089099734306No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA74790.1297846934580636No Hit
ATCACATTGCCAGGGATTTCCAGATCTCGTA74770.12974998702847193No Hit
CACCCGTAGAACCGACCTTGCGAATCTCGTA74730.1296805741692886No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA73840.12813613805245913No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT72500.12581080726981697No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG72190.12527285761114604No Hit
TACCCTGTAGATCCGAATTTGTGTATCTCGT71780.12456137580451672No Hit
CAAAGTGCTTACAGTGCAGGTAGAATCTCGT69830.12117749891932855No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA68620.1190777599290323No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGTA66660.11567652982904827No Hit
CTACAGTCCGACGATCTGGTCAAATCTCGTA66360.11515593338517317No Hit
TGAGGTAGTAGGTTGTGTGGTTAAATCTCGT65510.11368091012752704No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT64030.1111126343377432No Hit
CGGTGGATCACTCGGCTCGTGCGATCTCGTA63120.10953349179132203No Hit
TCTACAGTCCGACGATCTGGTCAAATCTCGT63040.10939466607295535No Hit
GTGGGCACATTACCCGTCTGACCATCTCGTA60330.10469194486328356No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA59840.10384163733828755No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT59260.10283515088012903No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC59090.10254014622859979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)