FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783553
Sequences flagged as poor quality0
Sequence length31
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA71234539.939659769011634Illumina Single End Adapter 1 (95% over 24bp)
TACCCTGTAGATCCGAATTTGTATCTCGTAT428962.4050869248068323No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT339501.9035038487782534No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA330381.852369960410484Illumina Single End Adapter 1 (95% over 23bp)
TATTGCACTTGTCCCGGCCTGTAATCTCGTA282301.5827956892786477No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT222331.2465567325445333No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT208591.1695194928325652No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA208391.1683981356315174No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA207051.1608850423844987No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT202271.1340846052794618No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA194311.0894545886777685No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA172410.9666659751630594No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT128200.7187899658714936No Hit
TCTACAGTCCGACGATCTGGTCAAATCTCGT121820.683018671158076No Hit
CTACAGTCCGACGATCTGGTCAAATCTCGTA117150.6568349805136152No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG89750.5032090439700979No Hit
TTCTACAGTCCGACGATCTGGTCAAATCTCG89330.500854193847898No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA85970.48201539287029876No Hit
GTTCTACAGTCCGACGATCTGGTCAAATCTC73490.4120427035249303No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT73380.41142595706435414No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT69520.389783763084136No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA69350.38883060946324555Illumina Paired End PCR Primer 2 (95% over 24bp)
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT64770.36315152955925617No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT62280.3491906324062139No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT61280.34358384640097606No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT60740.3405561819581476No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT56940.3192503951382437No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG55270.3098870625094965No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA54230.3040560050640491Illumina Single End Adapter 1 (95% over 24bp)
TGTAAACATCCCCGACTGGAAGATCTCGTAT54080.30321498716326345No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT53600.30052372988074927No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA52040.29177714371257824No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA49640.27832085730000733No Hit
TACAGTCCGACGATCTGGTCAAATCTCGTAT49250.27613421075796457No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT47890.2685089817908411No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG45900.2573514776404177No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG43850.24585756632968014No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT42160.23638209798082813No Hit
CAGTCCGACGATCTGGTCAAATCTCGTATGC41410.23217700847689976No Hit
AACATTCATTGCTGTCGGTGGGAATCTCGTA38330.21490810758076714No Hit
ACAGTCCGACGATCTGGTCAAATCTCGTATG37090.20795569293427218No Hit
TCCTGTACTGAGCTGCCCCGAATCTCGTATG37040.20767535363401032No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT36430.2042552141708152No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC35550.1993212424862059No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT35100.19679818878384886No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC33420.18737878829504928No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG30050.16848391945739769No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC29460.16517591571430734No Hit
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA27960.15676573670645055No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAACA27210.15256064720252216Illumina Single End Adapter 1 (95% over 24bp)
CGCGACCTCAGATCAGACGTGGATCTCGTAT27020.15149535786152696No Hit
AGTTCTACAGTCCGACGATCTGGTCAAATCT26790.15020579708032225No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGTA25390.14235629667298924No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA24730.13865581790953227No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT24480.1372541214082228No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA24440.1370298499680133No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG23940.13422645696539434No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA23330.13080631750219926No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA22590.12665729585832325No Hit
GTTCTACAGTCCGACGATCTGGTAAATCTCG22550.12643302441811372No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT22250.12475098861654238No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT22190.12441458145622811No Hit
AACATTCATTGCTGTCGGTGGGATCTCGTAT22060.12368569927554718No Hit
ATTTCGTATGCCGTCTTCTGCTTGAAAAAAA21340.11964881335177592Illumina Paired End PCR Primer 2 (95% over 24bp)
TACAGTACTGTGATAACTGAATATCTCGTAT21300.1194245419115664No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG20770.11645294532879034No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA20750.11634080960868558No Hit
CTCGTATGCCGTCTTCTGCTTGAAAAAAAAA20520.11505124882748088Illumina Single End Adapter 1 (100% over 22bp)
AGCAGCATTGTACAGGGCTATGATCTCGTAT19970.11196751652460006No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG19790.11095829504365723No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAATAA19300.10821096990109068Illumina Single End Adapter 1 (95% over 24bp)
CTTTCAGTCGGATGTTTACAGCATCTCGTAT19240.1078745627407764No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA18630.10445442327758132No Hit
ATCTCGTATGCCGTCTTCTGCTTGTAAAAAA18180.10193136957522429Illumina Single End Adapter 1 (95% over 24bp)
GTACAGTACTGTGATAACTGAATCTCGTATG18110.10153889455485764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)