FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2611954
Sequences flagged as poor quality0
Sequence length31
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA88928834.046847685679Illumina Single End Adapter 1 (95% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTAT1065054.0775986100827195No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT890093.407755266746658No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA555372.1262625605198253No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA409401.567408920677776Illumina Single End Adapter 1 (95% over 23bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT282551.0817571825537509No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG257900.9873833918974071No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT247560.9477961709892287No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT246270.9428573397540693No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG244120.9346259543621366No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA220240.8432001482415081No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA202570.7755496459738571No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT141890.5432331503541027No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG138250.5292972234579936No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA127870.48955686049601177No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT126330.483660891424581No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT126290.4835077493707776No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT126030.48251232602105554No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC120120.4598855875716035No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT112420.4304057422144494No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT110280.4222126423359676No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT105590.40425673652751926No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG101500.3885979615261218No Hit
CTACAGTCCGACGATCTGGTCAAATCTCGTA88290.3380227982575497No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC86400.33078683621533916No Hit
TCTACAGTCCGACGATCTGGTCAAATCTCGT83440.31945432423388775No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT80220.3071263889027142No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT79030.3025704128020631No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT78830.30180470253304614No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT76440.2926544648182931No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATGC71950.2754642692788617No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA68140.2608774886540881Illumina Single End Adapter 1 (95% over 24bp)
GTTTGTGATGACTTACATGGAATCTCGTATG67420.25812093168562694No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT64970.24874098089016883No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT62830.24054788101168703No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC60650.2322016390794019No Hit
TTCTACAGTCCGACGATCTGGTCAAATCTCG60440.23139764329693402No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT58260.22305140136464882No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCG52290.20019494983449174No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA52070.19935266853857303Illumina Paired End PCR Primer 2 (95% over 24bp)
AACATTCAACGCTGTCGGTGAGAATCTCGTA50220.19226984855016588No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT48480.1856081692097181No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA46730.17890820435581944No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG45820.17542422263179214No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT45760.17519450955108704No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG44600.17075338999078848No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT43150.16520199054041534No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA42140.16133515368187953No Hit
TACAGTCCGACGATCTGGTCAAATCTCGTAT38380.14693980062436016No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG37030.14177125630849546No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT36180.13851698766517326No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTATG34290.13128102562296273No Hit
CAGTCCGACGATCTGGTCAAATCTCGTATGC33010.126380479901254No Hit
GTTCTACAGTCCGACGATCTGGTCAAATCTC32930.1260741957936472No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG32000.12251364304271821No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT31110.1191062323455926No Hit
ACAGTCCGACGATCTGGTCAAATCTCGTATG30370.11627310435022975No Hit
ATCTCGTATGCCGTCTTCTGCTTGTAAAAAA30280.11592853472917211Illumina Single End Adapter 1 (95% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC30040.11500968240635173No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAACA29850.11428225765078558Illumina Single End Adapter 1 (95% over 24bp)
TCTCCCAACCCTTGTACCAGTGAATCTCGTA29780.11401425905662964No Hit
CGCGACCTCAGATCAGACGTGGCGACATCTC29280.11209998338408717No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG29200.11179369927648036No Hit
ATTCTACAGTCCGACGATCTGGTCAAATCTC27280.10444288069391729No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT27200.10413659658631048No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG26440.10122689756404593No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT26260.10053775832193064No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA26150.10011661767397129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)