FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8521587
Sequences flagged as poor quality0
Sequence length31
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA135993715.95872928364165Illumina Single End Adapter 1 (95% over 24bp)
TACCCTGTAGATCCGAATTTGTATCTCGTAT109286112.824618231322404No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT4277895.020062577545708No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT2289172.6863188746415427No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1531091.7967193200045954No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG1502521.7631927010778627No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT1324101.5538185551587984No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT1309761.5369907037268997No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1077831.2648230898775075No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA989781.1614972657088405No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT865521.0156793564391233No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA805340.9450587079613222No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC653520.7668994050051945No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT639240.7501419629935128No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG609250.7149489877883075No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT600450.7046222728231255No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA569380.6681619280540115Illumina Single End Adapter 1 (95% over 23bp)
AACATTCAACGCTGTCGGTGAGTATCTCGTA558820.6557698700957932No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT500500.5873319136447237No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGTA493760.5794225887736638No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT448820.5266859330310187No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT444820.5219919716832088No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG434510.5098932863092286No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT433140.5082856045476036No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA412020.48350148863116693No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG406200.4766717748701034No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT397430.46638026461503007No Hit
TACCCTGTAGATCCGAATTTGTGTATCTCGT387740.4550091432499604No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT375340.4404578630717494No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG373280.43804047297762727No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT362300.4251555490778889No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT355090.4166946837484614No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA354760.4163074319372671No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA347490.40777615718762245No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT313550.36794789515145476No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT301740.35408897427204583No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT295260.34648475688859365No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA284350.3336819773124419No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA266240.31243006731023226No Hit
TACCCTGTAGATCCGAATTTGTGATCTCGTA263540.30926164340046053No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTAT258980.30391052746395714No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG256660.30118802988222737No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC254220.29832471346006323No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG247750.2907322309799806No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA246500.28926536805879No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA245690.2883148408858585No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA232250.2725431307572169No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT215850.25329788923119606No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT191230.2244065571354256No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT181540.2130354357703559No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA181130.2125543047322054Illumina Paired End PCR Primer 2 (95% over 24bp)
TGTCAGTTTGTCAAATACCCCAATCTCGTAT180120.2113690794918834No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT179650.2108175390335157No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA176020.20655776911037818No Hit
TCGTATCCCACTTCTGACACCAATCTCGTAT166010.19481113083748366No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT157450.18476605355317033No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG155250.18218437481187483No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC145080.17024997808506795No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT135320.1587967123964116No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT124470.14606434224047704No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG124000.14551280178210937No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA120180.14103006869495083Illumina Single End Adapter 1 (95% over 24bp)
CATTGCACTTGTCTCGGTCTGTATCTCGTAT119680.1404433235264746No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG117870.13831930601659057No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT106140.12455426436413781No Hit
TGAGAACTGAATTCCATAGGCTGATCTCGTA102740.12056439721849932No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT101110.11865160796926674No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT97340.11422754939895585No Hit
TAGGTAGTTTCCTGTTGTTGGGATCTCGTAT97270.11414540507536917No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG94470.11085963213190218No Hit
TCGTATCCGGCTCGAAGGACCAATCTCGTAT93440.10965093708484112No Hit
TCTACAGTCCGACGATCTGGTCAAATCTCGT92580.10864173539506197No Hit
TCGTATCCCACTCCTGACACCAATCTCGTAT92430.10846571184451911No Hit
GTAAACATCCTTGACTGGAAGCTATCTCGTA91520.10739783563789233No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC91330.10717487247387136No Hit
ATACCCTGTAGATCCGAATTTGTATCTCGTA86140.10108445762508791No Hit
CTACAGTCCGACGATCTGGTCAAATCTCGTA85340.10014566535552591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)