FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10508224
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA141637113.47869059509961Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT3674883.4971466158315616No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT2823282.686733743018801No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT2752312.6191961648324207No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA2628572.5014407762910267No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA2609382.483178889220481No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA2281522.171175643001139No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT2269672.1598987611988476No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT2008661.91151235451395No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1629351.5505474569251665No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT1508251.435304386354916No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1499001.426501757099963No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT1378871.3121817730569885No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA1255641.194911718669111No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT1221171.1621088397049777No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT1019100.9698118349970462No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT984930.9372944467114518No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC893200.8500009135701713No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG778330.7406865327575811No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA772630.7352622098653397No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT752310.7159249745722969No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT654600.6229406605721385No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT616850.5870164168559787No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT597000.568126450292647No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG537160.5111805762800641No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA521280.49606860302939865Illumina Single End Adapter 1 (95% over 23bp)
TATTGCACTTGTCCCGGCCTGTATATCTCGT511510.486771123264978No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC502810.47849189358734645No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC483450.46006822846562845No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC427320.40665292251097807No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT374650.3565302757154777No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT373990.35590219622269187No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT369580.3517054832481683No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA363230.3456625972190924No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT347720.3309027291386252No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG340800.3243174108203251No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG329140.31322133978110855No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG313550.2983853408530309No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT309590.29461686389631586No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA300770.28622343794726873No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT300240.2857190710818498No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG287990.27406153504150654No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG266100.2532302318641095No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT263290.25055613584179404No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA261860.249195296940758No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT247710.23572965326966763No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTATG224490.2136326747507476No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT219810.2091790201655389No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA212540.20226062938894337No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT211750.2015088372687906No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG210750.20055720167366053No Hit
TGTAAACATCCCCGACTGGAAGAATCTCGTA209140.19902506836550113No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG207260.19723599344665665No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT203050.1932296075911591No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA200750.19104084572235994No Hit
TGTCAGTTTGTCAAATACCCCAGATCTCGTA199020.18939451614278494No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT197740.18817642258101847No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT194680.18526441765992047No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG187880.17879329561303603No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA180880.1721318464471256No Hit
AACATTCAACGCTGTCGGTGAATCTCGTATG175310.16683123618225115No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG171910.16359567515880896No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCAT168130.1599984926092173No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT167810.15969396921877568No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG157280.14967324640205615No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG156370.14880725801048778No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT155260.14775094249989343No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT150690.14340196783014905No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG150480.1432021243551717No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA150170.1429071173206814No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGTA148740.14154627841964543No Hit
TAGCACCATTTGAAATCGGTTATCTCGTATG147540.14040431570548934No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA147070.1399570469757782No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA142750.13584598120481634No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG142610.13571275222149815No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG141850.13498950916919927No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC139970.13320043425035477No Hit
AACATTCATTGCTGTCGGTGGGAATCTCGTA139640.13288639450396184No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA137700.13104022144940952Illumina Single End Adapter 1 (95% over 24bp)
TCGTATCCGGCTCGAAGGACCAATCTCGTAT135670.12910840119129552No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTAT131720.12534944059053177No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT128090.12189500338020964No Hit
AATGACACGATCACTCCCGTTGAATCTCGTA126960.12081965515771266No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT125630.11955397981618969No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA125580.11950639803643318No Hit
TGTAAACATCCTACACTCAGCTATCTCGTAT122010.11610905896181885No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC118510.11277833437886364No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT117350.11167443708851278No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCATC110540.10519379868567705No Hit
TATTGCACTTGTCCCGGCCTGTGAATCTCGT109030.10375682893703066No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA107430.10223421198482256No Hit
TGTCAGTTTGTCAAATACCCCAATATCTCGT106230.10109224927066648No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA106130.10099708571115347No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)