FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16481462
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGAATCTCGTAT177779410.786628030935605No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA166244710.086769001439313Illumina Single End Adapter 1 (95% over 24bp)
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT7693124.667741247712127No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA7154834.341137940311364No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA5239663.179123308356989No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT3168431.9224204745913926No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT2928801.7770268195867573No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA2722821.6520500426479154No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT2316781.4056884031283146No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT2164391.3132269455221872No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT2137171.2967114203824879No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1462430.8873181274816517No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1446310.8775374417633581No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT1221880.7413662695700175No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG1219630.7400010994170299No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT1137960.6904484565750295No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA1069480.648898744540988No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG1024980.6218987126263434No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT929140.5637485315319721No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA826800.5016545255511919No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC810600.491825300449681No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT789060.4787560715184126No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG787350.477718542202142No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT775670.47063179225241064No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT762050.46236796225965876No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT748310.4540313231920809No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT683920.41496318712502567No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC683440.41467195082572167No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG643540.39046293344607413No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA634400.38491730891349324Illumina Single End Adapter 1 (95% over 23bp)
TACAGTACTGTGATAACTGAATATCTCGTAT626080.37986921305889004No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC624220.37874067239908693No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT586080.3555995214502209No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG557170.3380586018400552No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG484280.2938331563061578No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG470160.28526595516829756No Hit
AATGACACGATCACTCCCGTTGAATCTCGTA439690.26677851758539384No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA424320.2574528885847627No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT405500.24603399868288384No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTAT404480.24541512154686276No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG383130.2324611736507356No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT375790.22800768524054482No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA371700.22552610927355837No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG364790.2213335200481608No Hit
TGTCAGTTTGTCAAATACCCCAGATCTCGTA341640.20728743602964347No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC340810.20678383992876362No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT339260.20584338937892765No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT334620.20302810515232203No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG328050.19904180830559812No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG327980.19899933634528297No Hit
TAGCTTATCAGACTGATGTTGACAATCTCGT310550.18842381822680537No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT310060.18812651450459916No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT307350.18648224289811183No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGTA298790.18128852889385663No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT297750.18065751691203122No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT297090.18025706700048819No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTATG288520.1750572855733308No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT283080.1717566075145518No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG281050.17052492066541183No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT267570.16234603459329033No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC267350.16221255128944265No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGTA265260.1609444599028897No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA264530.16050153803103148No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA260870.15828086124883825No Hit
TAGCTTATCAGACTGATGTTGACTATCTCGT255880.15525321722065674No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT255070.15476175596558123No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA253600.1538698447989626No Hit
TTCACCACCTTCTCCACCCAGCATCTCGTAT245420.14890669286498978No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG234770.1424448874741816No Hit
GGATATCATCATATACTGTAAGATCTCGTAT227770.1381976914426645No Hit
TCGTATCCGGCTCGAAGGACCAATCTCGTAT223840.13581319424211274No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTAT220990.13408397871499506No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG213540.12956374865288042No Hit
TCGTATCCCACTTCTGACACCAATCTCGTAT212960.12921183812455472No Hit
GTAAACATCCTTGACTGGAAGCTATCTCGTA210090.12747048775163272No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA208580.12655430689340544No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA207410.12584441841385188No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT206730.1254318336565045No Hit
TGTAAACATCCTTGACTGGAAGCTATCTCGT201080.12200373971677997No Hit
TTCAAGTAATTCAGGATAGGTTATCTCGTAT200880.12188239125873664No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG200870.12187632383583447No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA200780.12182171702971496Illumina Paired End PCR Primer 2 (95% over 24bp)
CATGCCTTGAGTGTAGGACCGTATCTCGTAT199940.12131205350593291No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT199040.12076598544473785No Hit
GTCAGTTTGTCAAATACCCCAAATCTCGTAT197810.12001969242777127No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG197370.1197527258200759No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTAT196040.11894575857408766No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC195370.11853924123964245No Hit
TTCACAGTGGCTAAGTTCCGCATCTCGTATG195170.1184178927815991No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG194970.11829654432355577No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT191170.1159909236207322No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA187320.11365496580339778No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGTA182970.111015636840955No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA180760.10967473637957603No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA180030.10923181450771782No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG174830.10607675459859081No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT174730.10601608036956915No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTAT174420.10582799025960198No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA174370.10579765314509113No Hit
TCGTATCCCACTCCTGACACCAATCTCGTAT174310.10576124860767813No Hit
ACCACAGGGTAGAACCACGGACATCTCGTAT174220.10570664180155863No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA172980.10495428136168988No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT169260.10269720004208364No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT168130.10201158125413876No Hit
TAGCACCATTTGAAATCGGTTATCTCGTATG168110.1019994464083344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)