FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12844257
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA135176210.524252200808501Illumina Single End Adapter 1 (95% over 24bp)
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT5660694.407175907489238No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT4049463.1527397808997435No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA3839392.989188086161777No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT3276652.551062315243303No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT2897702.256027732861465No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT2619012.039051383042242No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT2617742.038062614287459No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA2318901.8053983192643996No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1990241.5495174224558104No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1938981.5096085355501685No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG1375621.071000058625423No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT1190150.9266008925234055No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC1115960.8688396689664493No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT1062470.8271945975543779No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT895500.6971987558330544No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC879200.684508259216551No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT870360.6776258058368032No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG834720.6498779960569148No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT825070.642364910636715No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG820040.6384487635213154No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTAT800850.6235082340691253No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG791800.6164622834937047No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT730040.5683785368044255No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT705300.5491170100380272No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT592820.4615447978034074No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA584800.4553007620448578No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT577740.44980414203795516No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA563080.43839048066384845Illumina Single End Adapter 1 (95% over 23bp)
TAGCTTATCAGACTGATGTTGATCTCGTATG554080.43138345799215944No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA546360.42537298965599957No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC535890.4172214866146014No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG503950.39235434171085176No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT480960.3744552915750596No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT478300.3723843270965382No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA455870.35492126948253994No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT455850.35490569832104735No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC453270.3528970184884965No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT424540.3305290450043159No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG395320.30777957806356565No Hit
TAGCACCATTTGAAATCGGTTATCTCGTATG377050.29355532204003704No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT357940.27867707723381746No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT355920.2771043899230606No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTAT344720.2683845394871809No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC344530.2682366134530008No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG299110.2328745057032104No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG296240.23064004402901625No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT290320.22603098022719414No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG286660.22318145767404063No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT280290.21822204273863408No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA279250.21741234234101667No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG275600.2145706053686095No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTATG273080.21260863902053656No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG268380.20894941606976564No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA267570.20831878402931364No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT267540.2082954272870747No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT260600.20289223424912783No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT257720.20064998699418735No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT257660.20060327350970944No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT255650.1990383717796989No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA252430.1965314147793835No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTAT251810.19604870877311162No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA251440.1957606422854977No Hit
AATGACACGATCACTCCCGTTGAATCTCGTA251340.1956827864780345No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG235700.1835061381907883No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG230120.17916178413434114No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG226750.17653804342283091No Hit
GGATATCATCATATACTGTAAGATCTCGTAT226490.17633561832342656No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGTA225360.17545584769909228No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT225290.17540134863386805No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG222510.17323695718639076No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT221400.17237275772354912No Hit
TCCTGTACTGAGCTGCCCCGAGTATCTCGTA221200.1722170461086227No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTAT220640.1717810535868287No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT218690.17026286534129612No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA215480.16776369392172705No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA204530.15923848300450544No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG203960.15879470490196512No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG196190.15274530866207364No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA191070.14875909131995724No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT187900.14629106222337346No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT182170.14182992445573145No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT175770.13684715277808596No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA168920.131514029966856Illumina Paired End PCR Primer 2 (95% over 24bp)
TCTCCCAACCCTTGTACCAGTGATCTCGTAT166940.12997248497908442No Hit
TAGTGGTTAGTACTCTGCGTTATCTCGTATG166160.12936520968087137No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA160010.1245770775218839No Hit
GTTCAGTGGTAGAATTCTCGCCTATCTCGTA159130.12389194641620764No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGTA156450.1218054107761936No Hit
CTTCTCACTACTGCACTTGACTAGATCTCGT155440.12101906712081517No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC155400.12098792479782988No Hit
TAGCACCATTTGAAATCGGTATCTCGTATGC154550.1203261504343926No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT154020.11991351465483757No Hit
TGTCAGTTTGTCAAATACCCCAGATCTCGTA152600.11880796218885997No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA150200.1169394228097429No Hit
CTCGTACGACTCTTAGCGGTGGATCATCTCG144860.11278192269120743No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA144540.11253278410732516No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG144390.11241600039613034No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT143800.1119566511320974No Hit
TAGCACCATTTGAAATCGGTTAATCTCGTAT142110.11064088798596913No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT141680.11030610801387733No Hit
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA140600.10946526529327465No Hit
TCGTATCCCACTTCTGACACCAATCTCGTAT140060.10904484393297331No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC138950.10818064447013166No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA135660.10561918840459204No Hit
TAGTGGTTAGTACTCTGCGTTGTATCTCGTA132310.10301101885457446No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA129120.10052741859649804No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)