FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18031829
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTGTAGATCCGAATTTGTATCTCGTAT231528312.839978684358641No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA17929219.943090076996626Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT12342176.844657854730101No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA8171584.531753268068369No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT4839002.6835880043006175No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT4399432.4398135097665357No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT3540841.9636610351617685No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG3198111.7735915752084825No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT2907051.612177001013042No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA2041431.1321258647694585No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA1883441.0445085742550022No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA1655410.9180488568297759No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT1495200.8292004100083248No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT1409600.7817287974503307No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA1164720.645924492740032No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT1156630.6414379816933712No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA1125110.6239577804336986No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG1107310.6140863469812187No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT1088980.6039209888248164No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG1043190.5785270035557679No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT1020310.5658383295449397No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT979860.5434057743116353No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC950490.5271179091150432No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT894930.49630572694539193No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT839490.46556009376530805No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT810740.4496160650148135No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGTA802570.4450851879751078No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT796550.44174664699848254No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA792580.43954498459363156No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT774380.4294517211759273No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA748010.4148275807185173No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT746170.41380716287848557No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA743030.4120657976514751No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT683070.37881348586435687No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC670030.37158182899804565No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA661290.3667348442578953Illumina Single End Adapter 1 (95% over 23bp)
GTTTGTGATGACTTACATGGAATCTCGTATG623940.34602147125507904No Hit
TACCCTGTAGATCCGAATTTGTGTATCTCGT622700.34533379836288375No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA610970.338828634632682No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT572910.31772151344159266No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT566290.31405022751713096No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT540410.29969782876712064No Hit
TACCCTGTAGATCCGAATTTGTGATCTCGTA537590.2981339275122895No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG533480.29585462461961015No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG501810.2782912371229785No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT484900.26891337534312243No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT468920.2600512682324128No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTAT457540.2537402057217823No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA410860.22785264878010988No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT402240.22307221302952684No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG398970.22125875306381843No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT394890.21899608741853088No Hit
TATTGCACTTGTCCCGGCCTGTGAATCTCGT369720.20503743685679363No Hit
TCGTATCCGGCTCGAAGGACCAATCTCGTAT350590.19442841876994285No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA346100.1919383774103004No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG344260.19091795957026877No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT342840.19013046319372262No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT339060.18803417002235326No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG325810.18068605242429928No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT322520.17886150096032966No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG314830.17459681987889306No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC313770.1740089704710487No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG312720.173426666812335No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT301900.16742616625301848No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT285150.15813703645925214No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA283260.15708888987356745No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT270570.15005133422682748No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT268990.14917510586419158No Hit
TCGTATCCCACTTCTGACACCAATCTCGTAT256610.1423094684405004No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA253940.14082875342262838No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG245770.13629787638292265No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC234420.13000345111968398No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG228950.12696992634524207No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA217230.12047030836417093No Hit
TATTGCACTTGTCCCGGCCTGTAATATCTCG212210.11768634230060633No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT211220.11713731313667626No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT205420.11392077864092434No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG205050.11371558592309187No Hit
TGAGAACTGAATTCCATAGGCTGATCTCGTA193960.10756535013724897No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTAT190810.10581843916110784No Hit
ATTGCACTTGTCCCGGCCTGTATCTCGTATG186810.10360013950886514No Hit
TAGGTAGTTTCCTGTTGTTGGGATCTCGTAT186230.10327848605928994No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA180710.10021723253919498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)