FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19683996
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA18570869.434496938528133Illumina Single End Adapter 1 (95% over 24bp)
AGCAGCATTGTACAGGGCTATGAATCTCGTA8231874.182011619998297No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT8014704.071683412250236No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT6846423.4781657139129676No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT6437883.2706163931348087No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT6399553.2511437210208745No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA5029182.554958860995501No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT4997982.5391084208714534No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA4513622.293040498484149No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT2897911.4722163121756375No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC2781251.4129498908656555No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT2772001.408250641790417No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT2051241.0420851538478264No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT1952480.9919124145320899No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT1692970.8600743466926125No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT1690020.8585756672578068No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT1629090.8276215865924785No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG1627650.8268900278175223No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT1607670.816739649815007No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA1374030.6980442385783863No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT1200410.6098406035034756No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG1115790.566851364936266No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG1088900.5531905208678156No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG1071310.5442543272209566No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT1006060.5111055702307601No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT982200.4989840477512798No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA951220.4832453735511834No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT871060.44252193507862936No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC868200.44106897806725825No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG835000.42420248408910466No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA828840.4210730382184593No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT816820.41496655455528436No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG807550.4102571449415048No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG784040.39831343188649293No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT766760.38953472658702026No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA727680.36968103427779603No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT724010.36781657545551216No Hit
GGATATCATCATATACTGTAAGATCTCGTAT711260.3613392321355887No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT707170.3592614020039427No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA685280.34814069257075647No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT664910.33779218406669054No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT663170.33690821721361863No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT654050.3322750116388969No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA647360.32887631149691354No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA642440.32637681901581367Illumina Single End Adapter 1 (95% over 23bp)
ACCCTGTAGATCCGAATTTGTGAATCTCGTA637060.3236436341482695No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT599660.304643427076494No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG595950.3027586471771281No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT584500.2969417388623733No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG567470.2882900402946637No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG560530.28476433342091717No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT558510.2837381190282705No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG519550.2639453899502926No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG502930.25550198242267474No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT494220.2510770678880447No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT487850.24784093636271823No Hit
TAGCACCATTTGAAATCGGTTATCTCGTATG486650.2472313040502548No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG478530.24310612540258594No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC469320.23842719740442947No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT463100.23526726991816094No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA460240.23381431290678986No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT421800.21428575783087947No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT412990.20981004060354413No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA404020.20525303906788034No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA402110.20428270763720946No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT401810.20413029955909356No Hit
TCCAGCATCAGTGATTTTGTTGATCTCGTAT396100.20122946580562198No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA395680.20101609449625982No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC376210.1911248102265414No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG358680.18221909819530546No Hit
TGTCAGTTTGTCAAATACCCCAGATCTCGTA346020.17578747729881675No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA342470.17398398170777926No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTAT340050.17275455654431143No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC336480.17094090041473287No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT336060.17072752910537067No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT328200.1667344374587355No Hit
CACCCGTAGAACCGACCTTGCGAATCTCGTA325490.16535768448642235No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT322110.16364055347298384No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG321770.1634678243177859No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG305230.15506505894433226No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT302590.1537238678569128No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG280700.14260315842372656No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA271080.13771593938547844No Hit
TCCTGTACTGAGCTGCCCCGAATCTCGTATG246610.12528452048049593No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTATG236500.12014836824799192No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT236210.12000104043914661No Hit
TAGCAGCACATCATGGTTTACAATCTCGTAT233650.11870049150589138No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA227310.1154796007883765No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGTA225380.11449910881916456No Hit
TGTAAACATCCCCGACTGGAAGAATCTCGTA224550.11407744646971071No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA220080.11180656610578461No Hit
CTTATCAGATTGTATTGTAATTATCTCGTAT219090.11130361944800232No Hit
TTCACAGTGGCTAAGTTCCGCATCTCGTATG219040.11127821810164969No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT217920.11070922794335053No Hit
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA215080.10926643147052052No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGTA213390.10840786596380124No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC211360.10737657130188404No Hit
TATTGCACTTGTCCCGGCCTGTGAATCTCGT211270.10733084887844928No Hit
TAGCACCATTTGAAATCGGTTAATCTCGTAT199350.10127516790797966No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGTA199300.10124976656162703No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG199090.10114308090694592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)