FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9345011
Sequences flagged as poor quality0
Sequence length31
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA5943426.359992513652473Illumina Single End Adapter 1 (95% over 24bp)
AGCAGCATTGTACAGGGCTATGAATCTCGTA4486524.800978832448672No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT3441353.68255318265543No Hit
CGGCGGCGGCGGCGACTCTGGACGCGATCTC2352742.5176428363754737No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT2202082.3564231224553938No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT1998442.138510056328452No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT1857331.9875096990255015No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT1857011.9871672703221002No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT1719101.839591200053162No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT1574341.6846850153520418No Hit
GGCGGCGGCGGCGACTCTGGACGCGATCTCG1295901.3867292398050683No Hit
CGGCGGCGGCGGCGACTCTGGACGCATCTCG1264171.3527752936834425No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG1250541.3381899710979475No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT1218431.3038293908910326No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT1196841.2807261543084325No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG1127791.2068364606526412No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG1044071.117248551125301No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA973261.0414754996008029No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA898080.9610261560954825No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT800880.8570134374373662No Hit
CGGCGGCGGCGACTCTGGACGCGATCTCGTA768610.822481642878751No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT753750.8065801099645575No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT741700.7936855291021059No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG734910.7864196200518117No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA734360.7858310707178409No Hit
GGCGGCGGCGGCGACTCTGGACGCATCTCGT720990.7715239714538592No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG695370.7441082733878002No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC686660.7347877921170985No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTATG685960.7340387293284085No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC658080.7042046285445784No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT639860.6847075942446723No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAATCT610430.6532148544287427No Hit
GGATATCATCATATACTGTAAGATCTCGTAT586520.627629009746484No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA568980.6088596364413055No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT535540.5730758369358795No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT513990.5500154039412046No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT467150.4998924024808531No Hit
TAGCACCATCTGAAATCGGTATCTCGTATGC363300.38876358733018074No Hit
TAGCACCATTTGAAATCGGTATCTCGTATGC357490.38254636618405263No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTAT356710.3817116962195122No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA349830.3743494790963863No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT348220.372626634682399No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT339630.3634345641754729No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC337530.36118737580940247No Hit
GGCGGCGGCGGCGACTCTGGACGCGAATCTC314330.33636129481281507No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG297720.3185871049268963No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT296230.3169926712766844No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT282740.30255716124892734No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT279760.2993682939485036No Hit
CGGCGGCGGCGGCGACTCTGGACGATCTCGT267520.28627039604340754No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG260210.27844804035008625No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT251870.26952349226769234No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT243880.2609734755796435No Hit
CGGCGGCGGCGACTCTGGACGCGAATCTCGT238020.254702749948609No Hit
CGCGACCTCAGATCAGACGTATCTCGTATGC237510.25415700420256326No Hit
TGAGGTAGTAGATTGTATAGATCTCGTATGC236270.2528300929768836No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA230790.24696600143113795No Hit
TAGCACCATTTGAAATCGGTTATCTCGTATG229480.24556418392658927No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT227820.24378783502769552No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA225900.2417332628072883No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC220710.236179497274No Hit
GGCGGCGGCGACTCTGGACGCGATCTCGTAT220080.23550534076417887No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGATC214480.22951283845465778No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTAT205290.21967871412885442No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT191300.20470815925203298No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT189760.20306022111691466No Hit
AAACCGTTACCATTACTGAGATCTCGTATGC188030.2012089659391519No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGTA178740.19126783264353567No Hit
CGGCGGCGGCGACTCTGGACGCATCTCGTAT175560.1878649474034862No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT172320.184397856781549No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT168980.1808237571897989No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTAT168970.18081305629281763No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG168580.1803957213105474No Hit
CTTATCAGATTGTATTGTAATTATCTCGTAT165930.17755998361050618No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT163970.1754626078021738No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA159610.1707970167183324No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT155820.16674137676242437No Hit
GCGGCGGCGGCGACTCTGGACGCGATCTCGT154220.16502923324541835No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATGC150800.16136952647781794No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT150750.1613160219929115No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTAT150070.16058836099818397No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG144340.15445674702790613No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTAT140580.15043320976294197No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG138360.14805761063309608No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA135680.145189770242111No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTAT134380.1437986536345436No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA131020.14020315224883095No Hit
GCGACCTCAGATCAGACGTGGATCTCGTATG129200.1382555889982366No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC128760.13778474953105993No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT126880.13577298089857787No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC125710.1345209759517672No Hit
CGGCGGCGGCGACTCTGGACGCGAGATCTCG125660.13446747146686075No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA120790.12925613463697366No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA119940.1283465583935642No Hit
TAGCAGCACATCATGGTTTACAATCTCGTAT117370.12559642786937328No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA117040.1252432982689908No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA111920.11976443901457151No Hit
CGCGACCTCAGATCAGACGATCTCGTATGCC107610.11515235241563654No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCAT107400.11492763357902949No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG106820.11430698155411481No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT106500.1139645528507136No Hit
TGGCGGCGGCGGCGACTCTGGACGCGATCTC106370.11382544118995687No Hit
CGACCTCAGATCAGACGTGGATCTCGTATGC106030.11346161069259307No Hit
CTTCTCACTACTGCACTTGACTAGATCTCGT105550.11294796763749128No Hit
CTAGCACCATCTGAAATCGGTTATCTCGTAT104940.11229521292163273No Hit
CGTGTATTTGACAAGCTGAGTTGGACACTAT104890.11224170843672629No Hit
TACCACAGGGTAGAACCACGGAATCTCGTAT103330.11057236850764542No Hit
TTCAAGTAATCCAGGATAGGCTATCTCGTAT102910.11012293083443134No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG99940.1069447644309889No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTATG99360.1063241124060742No Hit
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA96710.10348837470603299No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT96530.10329575856036981No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA95590.10228987424412876No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA94910.10156221324940119No Hit
GGCGGCGGCGACTCTGGACGCGAATCTCGTA94800.10144450338260703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)