FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7866273
Sequences flagged as poor quality0
Sequence length31
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA144688818.393564525411207Illumina Single End Adapter 1 (95% over 24bp)
AACATTCAACGCTGTCGGTGAGTATCTCGTA2816003.579840160645327No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT2625233.337323787262405No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT2414663.069636662749945No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1937772.463390223044636No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA1909422.427350283927344No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT1810332.3013821157745222No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA1728302.1971014735949286No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1627382.0688069178377106No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT883931.1236960629258608No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT808461.0277548211204977No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT795811.0116735078988486No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT781160.993049694563105No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA748810.9519247552176233No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT550720.7001028314171146No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG500050.6356885910265255No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA496600.6313027783297123Illumina Single End Adapter 1 (95% over 23bp)
TCGTTTCCCGGCCAATGCACCAATCTCGTAT462890.5884489389066461No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT440680.5602144751396245No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA421320.5356030740351879No Hit
AACATTCATTGCTGTCGGTGGGATCTCGTAT315560.4011556680018606No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT309370.3932866301487375No Hit
AACATTCATTGCTGTCGGTGGGAATCTCGTA298390.37932830452235766No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT279450.35525082844188094No Hit
TGGTAGACTATGGAACGTAGGATCTCGTATG252680.32121946441472343No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT250600.318575264296065No Hit
TCGTATCCGGCTCGAAGGACCAATCTCGTAT247450.3145708266163659No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG247290.31436742660723827No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT244640.3109986139560628No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT241390.30686705127065894No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG239940.30502373868794025No Hit
AACATTCATTGCTGTCGGTGGGTATCTCGTA230410.29290872564427906No Hit
TCGGATCCGTCTGAGCTTGGCTATCTCGTAT214020.27207293720927306No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT204750.26028844918044414No Hit
ACCATCGACCGTTGATTGTACATCTCGTATG181320.2305030603438248No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT171990.21864229731157309No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA171610.2181592222898951No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT165870.21086224696244332No Hit
CAACGGAATCCCAAAAGCAGCTGATCTCGTA161680.20553570922341494No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG155890.1981751713931108No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT154980.1970183338411977No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT143240.18209385817146187No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT141000.17924625804367583No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA138920.17660205792501735No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG138220.1757121828850842No Hit
AACATTCATTGCTGTCGGTGGATCTCGTATG136110.1730298452647143No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT132620.16859318256561906No Hit
ACTCCCACTGCTTCACTTGACTAGATCTCGT130260.16559303243098733No Hit
TCGTATCCCACTTCTGACACCAATCTCGTAT129660.16483028239675893No Hit
CAACGGAATCCCAAAAGCAGCTATCTCGTAT128470.1633174948288726No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT127630.1622496447809528No Hit
TCGTTTCCCGGGCGGCGCACCAATCTCGTAT122740.1560332320019913No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT114940.14611748155702198No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT114920.14609205655588103No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT113460.1442360314725919No Hit
TCGTATCCCACTCCTGACACCAATCTCGTAT111870.14221474388188662No Hit
TCGGATCCGTCTGAGCTTGGCATCTCGTATG107300.13640513112118027No Hit
TGAGAACTGAATTCCATAGGCTGATCTCGTA102060.12974378082225216No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA100310.1275190932224193Illumina Single End Adapter 1 (95% over 24bp)
CGCGACCTCAGATCAGACGTGGCGATCTCGT98420.12511643061459982No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA94510.1201458428915447No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA94350.11994244288241712No Hit
AACATTCAACGCTGTCGGTGAATCTCGTATG94100.1196246303681553No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC92220.11723468026090629No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT90790.11541679267932858No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT89730.11406926761885838No Hit
CTTCTCACTACTGCACTTGACTAGATCTCGT87850.11167931751160938No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG86300.109708879923186No Hit
TCGGATCCGGCTCGAAGGACCAATCTCGTAT85400.10856475487184336No Hit
CTCCCACTGCTTCACTTGACTAGATCTCGTA83480.10612395476231247No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA82530.1049162672081175No Hit
ATCACATTGCCAGGGATTTCCAGATCTCGTA81270.10331449213623782No Hit
TTATGGTTTGCCTGGGACTGAATCTCGTATG81180.10320007963110357No Hit
CCTACTGTGGAATCTGAAGACAATCTCGTAT81160.10317465462996263No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGTA81070.10306024212482837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)