FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6813730
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA133723119.62553549964557Illumina Single End Adapter 1 (95% over 24bp)
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT2978114.370748474036981No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA2699503.961853492873947No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT2429523.5656241148387156No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT2087213.0632414257682647No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1457092.1384616061980735No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1385792.033819948838595No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT926391.3595930569599912No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA785021.1521149209023545No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT766121.1243768097649893No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT756031.1095684742424485No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA667910.9802413655956429No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA575740.8449703759908302Illumina Single End Adapter 1 (95% over 23bp)
TGTCAGTTTGTCAAATACCCCAATCTCGTAT442430.6493212968520913No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG427560.6274977141741748No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG404630.5938450745773607No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT386110.5666646609125985No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT386040.5665619271676453No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT360990.5297979227236771No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG356720.5235311642815316No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA350480.5143731847314174No Hit
TAGGTAGTTTCCTGTTGTTGGGATCTCGTAT329000.4828486012800625No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT299870.44009668713025024No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT284530.4175833207362194No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG278310.4084546936846632No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT275080.4037142651675367No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG245140.35977357482612315No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT227280.33356179361377686No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT208220.30558886248794714No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT201340.29549160298397503No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT195690.28719952214132344No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT193290.28367722231435644No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT185810.27269938785364256No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA183810.2697641379978367No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC182890.268413923064166No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA182080.26722514687256466No Hit
CAACGGAATCCCAAAAGCAGCTGATCTCGTA178860.2624993946047172No Hit
TCTTTGGTTATCTAGCTGTATGATCTCGTAT168890.2478671740735251No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA168140.24676645537759787No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTAT160120.23499610345581642No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT156160.2291843087413208No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA154190.22629308763335207No Hit
TAACTGGTTGAACAACTGAACCCATCTCGTA152620.22398891649654445No Hit
TTAATGCTAATCGTGATAGGGGTATCTCGTA151870.22288819780061728No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA150180.22040791167246132No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA143310.21032532841776824No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG139570.20483641118741128No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT139310.20445482870615653No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA137120.20124073011404914Illumina Paired End PCR Primer 2 (95% over 24bp)
ATCACATTGCCAGGGATTTCCAATCTCGTAT136120.19977310518614622No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT135570.19896591147579962No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT135190.1984082140031965No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG135090.1982614515104062No Hit
TCGTATCCGGCTCGAAGGACCAATCTCGTAT133780.19633886285485336No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA128640.18879527072543234No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTAT126650.1858746971189055No Hit
TCGTATCCCACTCCTGACACCAATCTCGTAT118010.17319441774182423No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT117600.17259269152138404No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT116540.17103700909780695No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATGC115680.1697748516598104No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA114200.1676027667665141No Hit
CAACGGAATCCCAAAAGCAGCTATCTCGTAT113420.16645801932274978No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG112010.16438866817440667No Hit
CGTGTATTTGACAAGCTGAGTTGGACACTAT109830.16118924583157831No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT108920.15985370714718664No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG105830.1553187461199666No Hit
TTAATGCTAATCGTGATAGGGGATCTCGTAT99330.14577918408859758No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA98160.14406206292295118No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG97810.14354839419818516No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGTA97080.142477028000816No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA96490.1416111292933533Illumina Single End Adapter 1 (95% over 24bp)
TTAATGCTAATCGTGATAGGGGTTATCTCGT95900.14074523058589056No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA93620.13739904575027187No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT92100.13516825585985942No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT89460.1312937260501957No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC88840.1303837985948959No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA87420.12829977119727373No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA85280.12515905385156148No Hit
ATCACATTGCCAGGGATTTCCATCTCGTATG83210.12212107025080243No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT82340.12084423656352687No Hit
CTCGTACGACTCTTAGCGGTGGATCATCTCG80910.11874553291662569No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG80340.11790898670772103No Hit
CGCGACCTCAGATCAGACGTGGCGACATCTC79310.11639733303198102No Hit
CTCGGCGTGGCGTCGGTCGTGGATCTCGTAT78130.11466553561705556No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG77300.11344740692689613No Hit
TTCACAGTGGCTAAGTTCCGCATCTCGTATG77070.11310985319347847No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC75010.11008654584199844No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT71790.10536079357415101No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT71620.10511129733640752No Hit
TAACTGGTTGAACAACTGAACCATCTCGTAT71470.10489115359722208No Hit
GTAAACATCCTTGACTGGAAGCTATCTCGTA71470.10489115359722208No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT70600.10361431990994655No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT70520.1034969099157143No Hit
TCGTATCCCACTTCTGACACCAATCTCGTAT69740.10235216247195002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)