FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10725518
Sequences flagged as poor quality0
Sequence length31
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA140968313.143262637757916Illumina Single End Adapter 1 (95% over 24bp)
CATTGCACTTGTCTCGGTCTGAATCTCGTAT3641433.3951087490599523No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA3500713.263907626652624No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT3272343.050985509511056No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT2361752.2019915494990547No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT1973671.8401628713876568No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1687711.5735463778998833No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA1584401.477224689753912No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1555861.4506152523355982No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT1088821.0151677522707994No Hit
GGAATACCGGGTGCTGTAGGCTTTATCTCGT948040.8839106885094034No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT737310.6874353294638078No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT663400.6185249048111243No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT632230.5894633713728326No Hit
AATACCGGGTGCTGTAGGCTTTATCTCGTAT603190.5623877560039524No Hit
AATGACACGATCACTCCCGTTGAATCTCGTA586690.5470038836352706No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA571540.5328786917331172Illumina Single End Adapter 1 (95% over 23bp)
TTTGGCAATGGTAGAACTCACAATCTCGTAT571410.53275748546597No Hit
GAATACCGGGTGCTGTAGGCTTTATCTCGTA544570.5077330530795809No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA543770.5069871683586751No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT526970.4913235892196536No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT522420.48708136986950185No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT522400.4870627227514792No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT502050.4680892801634383No Hit
ATACCGGGTGCTGTAGGCTTTATCTCGTATG480870.4483419821774575No Hit
GGAATACCGGGTGCTGTAGGCTTATCTCGTA467430.4358111188662403No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT448900.41853456401825995No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT425760.39695984846606014No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG419600.39121653611508556No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG416330.38816773231838314No Hit
TGTCAGTTTGTCAAATACCCCAATATCTCGT413510.3855384886771902No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT385450.35937658209141976No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT374520.3491859320920444No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC373620.3483468117810254No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT353890.32995142985168646No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC334680.3120408729909362No Hit
TACCGGGTGCTGTAGGCTTTATCTCGTATGC331360.3089454513991772No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT327440.3052906162667388No Hit
TTCACCACCTTCTCCACCCAGCATCTCGTAT326670.30457270222286703No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT313700.2924800461851819No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT312450.29131460130876663No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT312310.2911840714826081No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA306640.2858976135231883No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTATG301890.28146892299281023No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG299840.27955759339548913No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT279990.2610503287580143No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT278130.25931614678190834No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT276230.2575446705697571No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA271070.25273371411991474No Hit
TGTCAGTTTGTCAAATACCCCAGATCTCGTA269620.25138179806327304No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG253690.23652936855823653No Hit
GAATACCGGGTGCTGTAGGCTTATCTCGTAT247050.2303385253747185No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG241070.22476303708594772No Hit
CTCGTACGACTCTTAGCGGTGGATCATCTCG240310.22405444660108723No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT237350.22129467313373583No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA236990.2209590250093282No Hit
GTCAGTTTGTCAAATACCCCAAATCTCGTAT235640.21970034454279969No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG235630.2196910209837884No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA226340.21102943466226992No Hit
TAGTGGTTAGTACTCTGCGTTATCTCGTATG225470.21021828502828488No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT225150.20991993113992258No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT208460.19435891115002557No Hit
AATACCGGGTGCTGTAGGCTTATCTCGTATG207390.1933612903358141No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT199940.18641523887237896No Hit
TGGGAATACCGGGTGCTGTAGGCTTTATCTC195950.18269513882686134No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG193650.1805507202542572No Hit
TGGGAATACCGGGTGCTGTAGGCTTATCTCG193500.18041086686908736No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA192570.17954377588103437No Hit
GTGGTTAGTACTCTGCGTTGTATCTCGTATG187090.17443446554282974No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG180030.16785203288083617No Hit
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA179780.1676189439055531No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA178920.16681711783057937No Hit
GTCGCTATCTCGTATGCCGTCTTCTGCTTGA178280.16622041005385474RNA PCR Primer, Index 31 (96% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT177440.16543723109690367No Hit
GGGAATACCGGGTGCTGTAGGCTTTATCTCG174820.1629944586359372No Hit
ACCGGGTGCTGTAGGCTTTATCTCGTATGCC169980.1584818560744572No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG169650.15817417862708355No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGTA166180.1549389036501547No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA165120.15395060639495456No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCAT162990.15196468832554288No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC161550.15062209582791247No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG159200.1484310594602517No Hit
CAACGGAATCCCAAAAGCAGCTGATCTCGTA156400.14582046293708145No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA155780.14524240227837948No Hit
ATACCGGGTGCTGTAGGCTTATCTCGTATGC155600.14507457821617567No Hit
GTGGTTAGTACTCTGCGTTATCTCGTATGCC155040.14455245891154161No Hit
GTCAGTTTGTCAAATACCCCAATCTCGTATG150990.14077641751195608No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTAT146190.13630110918652136No Hit
TCCTGTACTGAGCTGCCCCGAATCTCGTATG146160.13627313850948738No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT145240.1354153710804457No Hit
ACCACAGGGTAGAACCACGGACATCTCGTAT145190.1353687532853891No Hit
CAACGGAATCCCAAAAGCAGCTATCTCGTAT145120.13530348837230985No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT139960.13049253192246754No Hit
TGTCAGTTTGTCAAATACCCCAAAATCTCGT132920.12392874637849659No Hit
CTCGTACGACTCTTAGCGGTGGAATCTCGTA128280.11960261499724303No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTAT127260.11865161197808814No Hit
ATCACATTGCCAGGGATTTCCAATCTCGTAT126740.11816678690949939No Hit
ACCACAGGGTAGAACCACGGACAATCTCGTA125380.11689878288395955No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC123800.11542566056017062No Hit
TAACTGGTTGAACAACTGAACCCATCTCGTA122260.11398983247242697No Hit
CGTGTATTTGACAAGCTGAGTTGGACACTAT120410.11226497405533234No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT120070.11194797304894738No Hit
TAGCAGCACATCATGGTTTACAATCTCGTAT119110.11105291138386045No Hit
TACCTGGTTGATCCTGCCAGTATCTCGTATG117570.1096170832961168No Hit
TGTCAGTTTGTCAAATACCCCAAGATCTCGT117040.1091229346685167No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA116430.10855419756882605Illumina Paired End PCR Primer 2 (95% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG115850.10801343114616935No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAT115690.1078642542019882No Hit
GTTTCCGTAGTGTAGTGGTCATCTCGTATGC114720.10695986897788994No Hit
TTCAAGTAATCCAGGATAGGCTATCTCGTAT113840.10613939578489356No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT113730.10603683663576902No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA112690.1050671864985915No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT112560.10494598023144429No Hit
ATCACATTGCCAGGGATTTCCAGATCTCGTA111040.1035287992617233No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGTA110790.10329571028644025No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA109620.10220485388211552No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGT107340.10007908242753404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)