FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7332214
Sequences flagged as poor quality0
Sequence length31
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA130003617.730469950822496Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT2042042.785025096103305No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT1725102.3527682088929756No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1720582.3466036315906766No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA1473652.009829500339188No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT1424581.942905648962237No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1107581.5105669310797532No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA882381.2034291415935214No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA878951.1987511548353609No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT851791.1617091372401296No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT799821.0908301367090485No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT724180.9876689360130515No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA594790.811201091512059Illumina Single End Adapter 1 (95% over 23bp)
CGCGACCTCAGATCAGACGTGGCATCTCGTA530090.7229603500388833No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT505740.6897507355895505No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT455330.6209993325344841No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT417990.5700733775637209No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA366020.49919437703263975No Hit
TGTCAGTTTGTCAAATACCCCAATATCTCGT360360.49147501695940676No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT348940.47589991235935014No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC324290.44228114454924533No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT291370.39738338242719046No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT280130.382053769843597No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT280050.3819446622807245No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG271910.3708429677584424No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT269350.36735152574652075No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT266320.3632190768027229No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG263960.36000040369798264No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT252260.344043422627872No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT226130.30840616490462497No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT214570.2926401220695413No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG210940.28768936640419934No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT206600.28177028111836344No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT206500.28163389666477273No Hit
AATGACACGATCACTCCCGTTGAATCTCGTA198660.27094135550326276No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA195980.2672862521470322No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT188570.2571801641359622No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT187320.2554753584660786No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG180640.24636487696622056No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG180340.2459557236054485No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT170980.23319013874936No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT169610.23132167173516757No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT162240.2212701375055338No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG160240.21854244843372No Hit
CTGTCACGCGGGAGACCGGGGTATCTCGTAT157520.2148327912960533No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT155300.21180505642633995No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA152080.2074134770207198No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT150710.20554501000652736No Hit
TTCACCACCTTCTCCACCCAGCATCTCGTAT148290.2022445062296327No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC147300.20089430013908488No Hit
GTCAGTTTGTCAAATACCCCAAATCTCGTAT145470.19839846463837527No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA144170.19662546674169631No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT143580.19582079846551123No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT139820.19069274301050132No Hit
TGTCAGTTTGTCAAATACCCCAGATCTCGTA139410.1901335667507795No Hit
CTCGTACGACTCTTAGCGGTGGATCATCTCG137660.18774683881294243No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA136280.18586473335339093No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT132760.18106400058699867No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA130700.1782544808430305No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTATG129080.17604505269486134No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA127990.17455846215072282No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT127000.17320825606017498No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGT125790.17155800417172767No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG121900.16625264892704986No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT118500.16161557750496644No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA115350.15731946721685974No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT115090.15696486763752396No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG114010.1554919155387445No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG113320.15455086280896876No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG113250.15445539369145525No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA102640.13998500316548318No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG102300.13952129602327482No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA99580.1358116388856081Illumina Paired End PCR Primer 2 (95% over 24bp)
TACAGTACTGTGATAACTGAATATCTCGTAT99210.13530701640732254No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA98530.13437960212290584No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG97000.1322929199829683No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG96770.13197923573970974No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA95330.13001529960800381Illumina Single End Adapter 1 (95% over 24bp)
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC94530.1289242239792783No Hit
AGTCTGTGATGAATTGCTTTGACTTCTGACA93510.12753310255265327No Hit
GTCAGTTTGTCAAATACCCCAATCTCGTATG90770.12379616852426839No Hit
GGTAGCGTGGCCGAGCGGTCATCTCGTATGC89280.12176404016576711No Hit
CGCGACCTCAGATCAGACGTATCTCGTATGC88630.12087754121742765No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCG87770.11970463491654772No Hit
GTCGCTATCTCGTATGCCGTCTTCTGCTTGA86410.11784980634771434RNA PCR Primer, Index 31 (96% over 28bp)
GTAGTCGTGGCCGAGTGGTTAAGGATCTCGT85570.11670417693755254No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT84900.11579040109849495No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA84450.11517667105733684No Hit
CGCGACCTCAGATCAGACGTGGCGACATCTC83930.11446747189866525No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCAT83640.11407195698325226No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA83490.11386738030286622No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATGC82200.11210802085154634No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTAT80950.11040321518166273No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT79790.10882115552001075No Hit
GGTAGCGTGGCCGAGCGGTCTATCTCGTATG78530.10710271140476806No Hit
GTCAGTTTGTCAAATACCCCAATATCTCGTA77560.10577978220493837No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC76840.10479781413908541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)