FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19615608
Sequences flagged as poor quality0
Sequence length31
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGCAATGGTAGAACTCACACTATCTCGT15737428.022907064619154No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA12604206.4255974120200605Illumina Single End Adapter 1 (95% over 24bp)
AGCAGCATTGTACAGGGCTATGAATCTCGTA11356485.789512106889575No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT10407965.305958398026714No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT8641974.405660023385459No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT7475793.8111436566228276No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT3696691.8845655969470843No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA3683271.877724106232139No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT2979241.5188109387177802No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG2889941.4732859669707918No Hit
TATGGCACTGGTAGAATTCACTATCTCGTAT2446501.247221090470405No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT2345981.1959761838633807No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG2072751.0566840446648402No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT2036681.0382956266254912No Hit
TTTGGCAATGGTAGAACTCACACTGATCTCG2007731.0235369711711204No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1995751.017429589743025No Hit
TTTGGCAATGGTAGAACTCACACTGGATCTC1919320.9784657197472544No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG1802630.9189773776066488No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT1787900.911468051359917No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC1778210.9065281076171587No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1728590.8812319251078018No Hit
GGATATCATCATATACTGTAAGATCTCGTAT1533550.7818009005889595No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA1344000.6851686677262311No Hit
TTTGGCAATGGTAGAACTCAATCTCGTATGC1259080.6418766117267433No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT1237590.6309210502167458No Hit
TATGGCACTGGTAGAATTCAATCTCGTATGC1026480.523297569975909No Hit
ACCGGGTGCTGTAGGCTTTATCTCGTATGCC1000060.5098287037546836No Hit
TTTGGCAATGGTAGAACTCACACATCTCGTA950240.48443056162215314No Hit
TATGGCACTGGTAGAATTCACTGATCTCGTA941150.4797964967489155No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG772360.39374767277160105No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT728790.371535768863244No Hit
TGAGGTAGTAGATTGTATAGATCTCGTATGC718290.3661828886466328No Hit
CGGCGGCGACTCTGGACGCGAGCATCTCGTA714670.3643374194671916No Hit
ATGGCACTGGTAGAATTCACTATCTCGTATG708970.36143157020674554No Hit
TCCTGTACTGAGCTGCCCCGAATCTCGTATG691140.35234186980082394No Hit
CTGGGTCGGTCGGGCTGGGGCGATCTCGTAT682590.3479830959101548No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT675050.3441392181165121No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT661960.3374659607798035No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT660940.33694596670161847No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC632000.3221924092284063No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT607610.30975843318239227No Hit
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA579180.2952648727482727No Hit
CGCGACCTCAGATCAGACGTATCTCGTATGC557440.2841818617093082No Hit
AAACCGTTACCATTACTGAGATCTCGTATGC549890.28033288593450684No Hit
ATGGCACTGGTAGAATTCACTGATCTCGTAT513460.2617609405734454No Hit
GGCGGCGACTCTGGACGCGAGCATCTCGTAT512860.26145506170392474No Hit
CGGCGGCGGCGACTCTGGACGCGAGCATCTC512510.26127663236337106No Hit
ACCGGGTGCTGTAGGCTTATCTCGTATGCCG501430.2556280692395566No Hit
TTTGGCAATGGTAGAACTCACATCTCGTATG487340.24844501378698025No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT456410.2326769580631913No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC435810.22217511687631603No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT409990.2090121295246112No Hit
CGGCGACTCTGGACGCGAGCATCTCGTATGC396540.20215534486619025No Hit
CTGGGTCGGTCGGGCTGGGGCATCTCGTATG377760.19258133625019422No Hit
CTGATCTCGGAAGCTAAGCAGGGTCGGATCT353180.1800504985621654No Hit
ATGGCACTGGTAGAATTCACTGAATCTCGTA344950.17585486006857395No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA337840.1722301954647544No Hit
TATGGCACTGGTAGAATTCACATCTCGTATG335410.1709913860431958No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG332050.16927846437388022No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT317860.16204442910971714No Hit
AAACCGTTACCATTACTGAGTTTAGATCTCG317300.1617589421648312No Hit
GTGATCGTATAGTGGTTAGTACTCTGCATCT300320.15310257015739712No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG298460.15215434566188313No Hit
TATGGCACTGGTAGAATTCACTGAATCTCGT296420.15111435750551294No Hit
GCGGCGACTCTGGACGCGAGCATCTCGTATG285250.1454199125512704No Hit
CGCGACCTCAGATCAGACGATCTCGTATGCC273580.13947056853909398No Hit
GGCGGCGGCGACTCTGGACGCGAGCATCTCG273230.13929213919854025No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA267770.13650864148590244No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT265400.1353004199512959No Hit
TCCTGTACTGAGCTGCCCCGAGTATCTCGTA259130.13210398576480525No Hit
TCGTCTGATCTCGGAAGCTAAGCAGGGTCGG259060.1320682998966945No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT257730.13139026840259044No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG254470.12972832654486163No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG234650.11962412788836319No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT232610.11858413973199301No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT227230.11584142586862463No Hit
CTTTCAGTCGGATGTTTACAATCTCGTATGC219790.11204852788656869No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA209710.10690976287862197No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA207950.10601251819469476No Hit
AAACCGTTACCATTACTGAGTTTAGTATCTC207060.10555879787157248No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC196360.10010395803178776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)