FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20000000
Sequences flagged as poor quality0
Sequence length31
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGAATCTCGTAT370723718.536185No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA257815412.890769999999998Illumina Single End Adapter 1 (95% over 24bp)
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT8764294.3821449999999995No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG8724934.362465No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA3920581.9602899999999999No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT3486631.7433150000000002No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA2095301.04765No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC2021701.01085No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG1989520.9947599999999999No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC1591550.7957749999999999No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT1489320.74466No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1467270.733635No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC1295800.6479No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG1203670.601835No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1120750.5603750000000001No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG911310.45565500000000003No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT873700.43685No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA854090.427045No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG839230.419615No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT741400.3707No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATGC693580.34679No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA692490.346245Illumina Single End Adapter 1 (95% over 23bp)
TCTCCCAACCCTTGTACCAGTGATCTCGTAT670240.33512No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA661230.330615No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGTA635030.317515No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT617770.308885No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT599000.2995No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC596170.29808500000000004No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT594430.297215No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT593450.29672499999999996No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG578310.289155No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG568640.28432No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG549380.27469No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC548000.27399999999999997No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT543300.27165No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG543260.27163No Hit
GGATATCATCATATACTGTAAGATCTCGTAT540270.270135No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC537720.26886000000000004No Hit
TAGCACCATCTGAAATCGGTATCTCGTATGC527180.26359No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT520140.26007No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT506520.25326No Hit
TCTTTGGTTATCTAGCTGTATGATCTCGTAT485970.24298499999999998No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT480140.24007No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT463530.231765No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG455960.22798No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT424870.212435No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTAT423730.21186500000000003No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT410480.20523999999999998No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT405360.20268No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGTA401000.20049999999999998No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA396110.198055No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT385730.192865No Hit
TGAGGTAGTAGATTGTATAGATCTCGTATGC384010.192005No Hit
TAGCTTATCAGACTGATGTTATCTCGTATGC381980.19099No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTATG379930.189965No Hit
CTTTCAGTCGGATGTTTACAATCTCGTATGC378720.18936No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT364970.182485No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT342870.171435No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT338610.169305No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG333140.16657No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT331440.16572No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA329810.164905No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTAT293070.146535No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATGC289030.144515No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTAT277300.13865No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCATC272660.13633No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG271630.135815No Hit
TAGCACCATTTGAAATCGGTTATCTCGTATG265520.13276No Hit
TAGCTTATCAGACTGATGTTGACAATCTCGT253860.12693No Hit
TAGCTTATCAGTCTGATGTTGAATCTCGTAT249860.12493000000000001No Hit
TAGCACCATCTGAAATCGGATCTCGTATGCC244520.12226No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTAT240440.12022000000000001No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA235850.117925Illumina Single End Adapter 1 (95% over 24bp)
TACCCTGTAGATCCGAATTTGTATCTCGTAT235170.117585No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG229090.114545No Hit
TAGCACCATTTGAAATCGGTATCTCGTATGC223100.11155No Hit
TCCTGTACTGAGCTGCCCCGAATCTCGTATG219870.109935No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCAT210040.10502No Hit
TAGCTTATCAGACTGATGTTGACTATCTCGT200570.100285No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT200460.10023No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)