FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13521940
Sequences flagged as poor quality0
Sequence length31
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA265232019.614936909940436Illumina Single End Adapter 1 (95% over 24bp)
AGCAGCATTGTACAGGGCTATGAATCTCGTA2979602.2035299668538686No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT2909992.1520506672859074No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT2483081.8363341354864762No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT2467601.8248860740396717No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA2121281.5687689784158192No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT1517481.1222354188822017No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA995660.7363292545300453No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT942760.6972076491982659No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC888510.6570876664147305No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT828920.6130185461553593No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT815750.6032788194593379No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT797390.5897008861154539No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA784680.5803013472918827Illumina Single End Adapter 1 (95% over 23bp)
AAACCGTTACCATTACTGAGTATCTCGTATG662080.4896338838953582No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA657930.48656479765477434No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA647570.4789031751361121No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT645710.47752763286924804No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC639860.4732013305783046No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT632140.46749209063196556No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC596910.4414381368353949No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA589350.4358472231055603No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT583930.4318389225214725No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT562920.4163012112167337No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT496120.36690001582613146No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT473960.35051183484026704No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG459080.3395074967053544No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG454620.3362091534202932No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT433230.32039041735135637No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT425880.3149548067806839No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT418640.3096005454838581No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA413530.3058215019442476No Hit
TGTCAGTTTGTCAAATACCCCAATATCTCGT392170.2900249520409054No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCG391260.2893519716845364No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT341110.2522640981989271No Hit
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA333190.2464069504819575No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT331320.2450240128265619No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG328660.24305683947717563No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT319030.23593508032131483No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG318440.23549875239795476No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG313340.23172710424687581No Hit
TCCTGTACTGAGCTGCCCCGAATCTCGTATG311540.23039593431120092No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT309380.22879853038839099No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT308100.22785192065635554No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC307900.22770401288572498No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT292860.21658134853430794No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT278000.2055918011764584No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT274530.20302560135601844No Hit
GGATATCATCATATACTGTAAGATCTCGTAT273850.2025227149358746No Hit
CGCGACCTCAGATCAGACGTATCTCGTATGC272410.20145777898733463No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT271240.20059251852914597No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG264410.19554146816211285No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA260900.19294568678754676No Hit
CGCGACCTCAGATCAGACGATCTCGTATGCC260570.19270163896600634No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTATG260500.1926498712462857No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT256670.1898174374387107No Hit
ACCGGGTGCTGTAGGCTTTATCTCGTATGCC239640.17722309076951975No Hit
CTCGTACGACTCTTAGCATCTCGTATGCCGT234670.1735475826693507No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT220750.16325320183346473No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT218890.16187765956660063No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA218100.16129342387261Illumina Single End Adapter 1 (95% over 24bp)
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC216370.16001402165665576No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG214600.15870503788657545No Hit
GGTAGCGTGGCCGAGCATCTCGTATGCCGTC211390.1563311181679552No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG210190.1554436715441719No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA206140.15244853918890336No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT201180.14878042647726583No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG198440.14675409001962736No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG193310.14296025570295387No Hit
AAACCGTTACCATTACTGAGTTTAGATCTCG193290.1429454649258908No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTATCT191830.14186573820028783No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT178940.13233308238314917No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAT176440.13048423525026734No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG174870.12932315925081758No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA174500.12904952987515106Illumina Paired End PCR Primer 2 (95% over 24bp)
GGGTGCTGTAGGCTTTATCTCGTATGCCGTC171430.12677914559597217No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT168700.12476020452686523No Hit
TCCTGTACTGAGCTGCCCCGAGTATCTCGTA163000.12054483306389469No Hit
TTTGGCAATGGTAGAACTCACACTGATCTCG156520.11575262129546501No Hit
CGCGACCTCAGATCAGACGTGGCGACATCTC153420.11346005085069154No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC152990.11314204914383587No Hit
CCTGTCTGAGCGTCGCTATCTCGTATGCCGT147400.10900802695471212No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA147370.10898584078911755No Hit
TTTGGCAATGGTAGAACTCACACTGGATCTC144140.10659713029343423No Hit
AAACCGTTACCATTACTGAGATCTCGTATGC143470.1061016392618219No Hit
AAACCGTTACCATTACTGAGTTTAGTATCTC141960.10498493559356128No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA141240.1044524676192913No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT136030.1005994701943656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)