FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10234736
Sequences flagged as poor quality0
Sequence length31
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA198438119.388687700395984Illumina Single End Adapter 1 (95% over 24bp)
TCTCCCAACCCTTGTACCAGATCTCGTATGC4179674.083808317088003No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT3524823.443977450908357No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG3327923.251593397230764No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA1361411.3301857517380027No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT1293641.2639700721151967No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1183671.156522259098818No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT1142301.1161010894663037No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT890780.8703497579224321No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA837370.8181647284307089No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG726540.709876639710101No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC698540.6825188260840338No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG689840.6740183625645058No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT686080.670344599020434No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA627360.6129713555874817Illumina Single End Adapter 1 (95% over 23bp)
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT565650.5526766884851744No Hit
TCTCCCAACCCTTGTACCAGTGAATCTCGTA505490.49389647178002444No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA492080.480794033182683No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC415480.40595087161994214No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT377920.36925231877011777No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC372820.3642692884310841No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCATC327390.31988123582279016No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT313640.30644659520284645No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG305080.29808292075144877No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG302610.2956695707637207No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT302590.2956500294682735No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCATC290770.2841011238589838No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT284620.2780921755089726No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT275260.26894684923968726No Hit
TCTCCCAACCCTTGTACCAGTGTATCTCGTA265970.25986991750446714No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT255200.2493469299061549No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCAT251630.2458588086688313No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT239980.23447600407084265No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG238270.23280522331010786No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT236140.23072407534498202No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC225530.2203574181102473No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA221480.21640030578219116No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTATG220490.2154330116575552No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT219530.21449502947609006No Hit
ATGCAGTGTGGAACACAATGAACTGAACATC215900.21094828435242494No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATGC211090.20624860279737553No Hit
TAGCACCATCTGAAATCGGTATCTCGTATGC201340.19672222126687003No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCG200440.19584286297174644No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG192560.18814359256555324No Hit
CTCGTACGACTCTTAGCATCTCGTATGCCGT192560.18814359256555324No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT188210.18389336080578922No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT182710.17851950455781174No Hit
TGTCGCTGATGCCATCACCGCAGCGCTCTGA182650.17846088067147017No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT181810.17764014626268815No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC180620.1764774391835803No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA173920.1699311052087714Illumina Single End Adapter 1 (95% over 24bp)
TAGCACCATTTGAAATCGGTTATCTCGTATG172370.1684166548116141No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG165730.16192894472314676No Hit
CTAGCACCATCTGAAATCGGTTATCTCGTAT165680.16188009148452878No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT165350.16155766010965011No Hit
CTCGTACGACTCTTAGCGGATCTCGTATGCC165330.16153811881420294No Hit
CGCGACCTCAGATCAGACGATCTCGTATGCC162240.15851898866761197No Hit
CACCATGATGGAACTGAGGATCTGAGGATCT154980.15142549842028166No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT146910.14354058570734018No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA144660.1413421899695312Illumina Paired End PCR Primer 2 (95% over 24bp)
CTCGTACGACTCTTAGCGGTGATCTCGTATG143070.13978865698147955No Hit
CTGTCACGCGGGAGACCGGGGTATCTCGTAT141360.13811787622074473No Hit
TAGCACCATTTGAAATCGGATCTCGTATGCC140200.13698448108480768No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTAT140110.1368965452552953No Hit
GAGACTCTGAAATCTGATTATCTCGTATGCC133140.1300864037919493No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT132550.12950993557625717No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG131850.12882599023560548No Hit
CTAGCACCATCTGAAATCGGTATCTCGTATG130880.12787823740641674No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT129320.12635401636153584No Hit
TAGCACCATTTGAAATCGGTATCTCGTATGC129040.12608043822527518No Hit
CGCGACCTCAGATCAGACGTATCTCGTATGC126370.1234716752830752No Hit
TGTCTCCCAACCCTTGTACCAGATCTCGTAT124130.12128305019298982No Hit
TCCTGTACTGAGCTGCCCCGAATCTCGTATG124060.12121465565892466No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA121780.11898694797794492No Hit
GTCGCTATCTCGTATGCCGTCTTCTGCTTGA121670.11887947085298536RNA PCR Primer, Index 31 (96% over 28bp)
TAGCACCATCTGAAATCGGATCTCGTATGCC120660.11789263543290224No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT120180.11742364434216965No Hit
CTCGTACGACTCTTAGCGATCTCGTATGCCG119860.1171109836150146No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT119730.11698396519460785No Hit
TTGTAGTGTGCTGATGATCTCGTATGCCGTC119390.1166517631720056No Hit
CCTACTGTGGAATCTGAAGACAATCTCGTAT117630.11493212917265282No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT116130.11346653201411351No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG110930.10838579519784389No Hit
CTTTCAGTCGGATGTTTACAATCTCGTATGC108120.10564024318751358No Hit
CACTAGATTGTGAGCTCCTGGAAATCTCGTA107740.10526895857401695No Hit
CTGTCTGAGCGTCGCTATCTCGTATGCCGTC106250.10381313206320124No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT105150.10273836081360574No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG104360.10196647964344172No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)