FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9454634
Sequences flagged as poor quality0
Sequence length31
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA221298623.406363482711228Illumina Single End Adapter 1 (95% over 24bp)
AGCAGCATTGTACAGGGCTATGAATCTCGTA2791822.9528588838023766No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1976442.0904458067863865No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT1932282.0437385519101006No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT1713841.812698407997602No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1267321.3404220618164595No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT780390.8254047697668677No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT770370.814806792097928No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA745730.7887454977104349No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA674540.7134490875056613Illumina Single End Adapter 1 (95% over 23bp)
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT528320.558794766672089No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC527010.5574092027253513No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT421850.4461833213215869No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA420190.4444275685341178No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT400970.42409891276595163No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT390540.41306728531215486No Hit
TGTCAGTTTGTCAAATACCCCAATATCTCGT364230.3852396613131719No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT356390.37694743128078784No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT330560.349627494834808No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT305240.3228469764138939No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT301240.31861624680553474No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA296860.31398359788438135No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATGC283370.2997154622801898No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT280810.2970077953308399No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT278630.2947020476942841No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT274310.2901328597172561No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT267880.28333196187181864No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT251570.26608116189373376No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCG246440.26065525117101307No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT239580.25339954989267693No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG237630.2513370692086018No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG232200.24559385376525417No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG224700.23766123574958056No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT222700.23554587094540094No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA222180.23499587609631423No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA221530.2343083825349559No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA218910.2315372546414806Illumina Single End Adapter 1 (95% over 24bp)
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA212760.22503250786862825No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCAT211290.2234777147375562No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC211250.2234354074414726No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA204340.21612682204303202No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA200290.2118432083145683No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG193160.20430193278766795No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG192060.20313848214536914No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT190990.20200676197513306No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAT187920.19875967700071734No Hit
TCCTGTACTGAGCTGCCCCGAATCTCGTATG187170.19796641519914995No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT182000.19249819718034564No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT180240.19063667615266758No Hit
TTTGGCAATGGTAGAACTCACACTGGATCTC177070.18728382293804285No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT177050.18726266929000107No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT174120.18416365985187794No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT173940.18397327701950175No Hit
CGCGACCTCAGATCAGACGATCTCGTATGCC170890.18074734569312784No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT168450.17816660063202872No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT159230.16841476888476065No Hit
TTTGGCAATGGTAGAACTCACACTGATCTCG157650.16674363068945874No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC155870.16486095601373887No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA154730.1636551980753565No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA152830.16164560151138582Illumina Paired End PCR Primer 2 (95% over 24bp)
TGAGGTAGTAGGTTGTATAGATCTCGTATGC152360.16114849078240362No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTATCT148550.15711872083044146No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG146450.15489758778605286No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC145530.15392451997613021No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT144720.15306779723043748No Hit
TCCTGTACTGAGCTGCCCCGAGTATCTCGTA142290.15049762899335925No Hit
ACCGGGTGCTGTAGGCTTTATCTCGTATGCC135670.1434957714915247No Hit
CTCGTACGACTCTTAGCATCTCGTATGCCGT133100.14077752771815388No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT131310.1388842762184131No Hit
CGCGACCTCAGATCAGACGTATCTCGTATGC130860.1384083191374727No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG129990.13748813544765456No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA128690.13611314832493782No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT123080.13017955004921397No Hit
TGTCAGTTTGTCAAATACCCCATCTCGTATG120750.12771515005234468No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT117410.12418249082936474No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA114140.12072386937453106No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT111590.11802677924920205No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG111520.11795274148105575No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT109540.11585853032491791No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG108160.11439892861003398No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA107980.11420854577765781No Hit
CCCCCCACTGCTAAATTTGACTGGCTTATCT103040.10898359471133415No Hit
GGTAGCGTGGCCGAGCATCTCGTATGCCGTC99460.10519709171185262No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT97080.10267980759487887No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC96780.10236250287425193No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT96000.10153751060062188No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG94630.10008848570975883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)